Iws1 (BC068184) Mouse Tagged ORF Clone
CAT＃: MG208664
- TrueORF®
Iws1 (tGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737)
ORF Plasmid: DDK
"BC068184" in other vectors (4)
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CNY 3420.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700069O15Rik; 3000008H23; AL117927; AW214353 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208664 representing BC068184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGAGTACTACAGCGGGGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG ATTCAGGGTCAGATGGTGAGGATGGTGTAACCGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCA CCACTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGGCCAAGATACATAATGGAACAGACTCTGAG AATGATGAACCTTCCAATGCTAATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAGGACAGTG ACTCTGACTCTGAGGAGCATGCAGAGTCTCCTGCTAGTGATTCTGAAAATGAACCTGTCAACCAACATGG GAGCGACTCTGAAAATGAAGAGCTGCTTAATGGGCATGCCAGTGACTCTGAAAAGGAAGAGGTTAGCAAG CATGCCGCCAGTGACTCTGAAGCTGAGGACACTCTGCAGCCTCAGGTCAGCGAGTCTGACAGTGAAGATC CCCCAAGGCCCCAGGCCAGTGACTCAGAAAATGAGGAGCCTCCCAAACCTCGGATAAGTGACTCGGAGAG TGAGGAGCTCCCCAAACCCCGAGTCAGTGACTCAGAAAGTGAGGACCCTCCAAGGCCCCAGGCCAGTGAC TCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCTGAAAGTGAGGACCCTCCAAGGCCCCAGG CCAGTGACTCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACACAAAGAGAAGCCAGACTCAGATGACAGT GATGGGGAGAATAAGAGGGAAGATTCTGAAGTGCAGAATGAGTCAGATGGCCATACAGACAGAAAAGGAC TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAAAGGCAAAAAATTGACAGTGATGATGATGAAGAAAA GGAGGGGGATGAGGAGAAAGTAGCAAAGCGGAAGGCTGCTGTGCTTTCTGATAGTGAGGATGACGCAGGC AACGCATCAGCAAAGAAGAGTCGAGTTGTCTGTGATGCTGATGACTCTGATAGTGATGTTGTATCGGACA AGTCAGGCAAACGAGAGACAACAGTAGCATCTGACAGTGAAGAAGAAGCCGGGAAAGAAGAGTCTTCTGT GAAGAAGAAGGAAGACAAGGACCTGTTTGGGAGTGACAGTGAGTCCGGCAATGAAGAAGAAAATCTTATT GCAGATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACGGGTTTTAACCAAGAAGATTTGG AGGAAGAGAAAAACGAAACACAGCTAAAGGAAGCAGAAGATTCTGATTCTGATGATAACATAAAGAGAGG CAAGCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAAAGCATGTGTGGCAAACGC AGACGAAATCGAGATGGGGGCACTTTTATTAGTGATGCAGATGATGTTGTGAGCGCCATGATTGTCAAGA TGAATGAAGCTGCTGAGGTAAGCTCTTCTAGGTTACCCTTCTCCCCTCCTGCCCTCAACCAAATTGATGG GGTGGCAAATCTTTCTCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208664 representing BC068184
Red=Cloning site Green=Tags(s) MDSEYYSGDQSDDGGATPVQDERDSGSDGEDGVTEQHSGSDTGSVDHHSENETSDREDGLAKIHNGTDSE NDEPSNANASDSESEELHRPKDSDSDSEEHAESPASDSENEPVNQHGSDSENEELLNGHASDSEKEEVSK HAASDSEAEDTLQPQVSESDSEDPPRPQASDSENEEPPKPRISDSESEELPKPRVSDSESEDPPRPQASD SESEELPKPRVSDSESEDPPRPQASDSESEELPKPRVSDSESEDPQKGPASDSEAEDASRHKEKPDSDDS DGENKREDSEVQNESDGHTDRKGLHSSDSEEEEPKRQKIDSDDDEEKEGDEEKVAKRKAAVLSDSEDDAG NASAKKSRVVCDADDSDSDVVSDKSGKRETTVASDSEEEAGKEESSVKKKEDKDLFGSDSESGNEEENLI ADIFGESGDEEEEEFTGFNQEDLEEEKNETQLKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMCGKR RRNRDGGTFISDADDVVSAMIVKMNEAAEVSSSRLPFSPPALNQIDGVANLSL TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC068184 |
| ORF Size | 1629 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068184, AAH68184 |
| RefSeq Size | 4353 bp |
| RefSeq ORF | 1631 bp |
| Locus ID | 73473 |
| Gene Summary | Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218462 | Iws1 (untagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737), (10ug) |
CNY 6940.00 |
|
| MR208664 | Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 4040.00 |
|
| MR208664L3 | Lenti ORF clone of Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 5040.00 |
|
| MR208664L4 | Lenti ORF clone of Iws1 (mGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 5040.00 |
