Iws1 (BC068184) Mouse Untagged Clone
CAT＃: MC218462
Iws1 (untagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737), (10ug)
CNY 6940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700069O15Rik; 3000008H23; AL117927; AW214353 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC068184
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGAGTACTACAGCGGGGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG ATTCAGGGTCAGATGGTGAGGATGGTGTAACCGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCA CCACTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGGCCAAGATACATAATGGAACAGACTCTGAG AATGATGAACCTTCCAATGCTAATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAGGACAGTG ACTCTGACTCTGAGGAGCATGCAGAGTCTCCTGCTAGTGATTCTGAAAATGAACCTGTCAACCAACATGG GAGCGACTCTGAAAATGAAGAGCTGCTTAATGGGCATGCCAGTGACTCTGAAAAGGAAGAGGTTAGCAAG CATGCCGCCAGTGACTCTGAAGCTGAGGACACTCTGCAGCCTCAGGTCAGCGAGTCTGACAGTGAAGATC CCCCAAGGCCCCAGGCCAGTGACTCAGAAAATGAGGAGCCTCCCAAACCTCGGATAAGTGACTCGGAGAG TGAGGAGCTCCCCAAACCCCGAGTCAGTGACTCAGAAAGTGAGGACCCTCCAAGGCCCCAGGCCAGTGAC TCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCTGAAAGTGAGGACCCTCCAAGGCCCCAGG CCAGTGACTCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACACAAAGAGAAGCCAGACTCAGATGACAGT GATGGGGAGAATAAGAGGGAAGATTCTGAAGTGCAGAATGAGTCAGATGGCCATACAGACAGAAAAGGAC TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAAAGGCAAAAAATTGACAGTGATGATGATGAAGAAAA GGAGGGGGATGAGGAGAAAGTAGCAAAGCGGAAGGCTGCTGTGCTTTCTGATAGTGAGGATGACGCAGGC AACGCATCAGCAAAGAAGAGTCGAGTTGTCTGTGATGCTGATGACTCTGATAGTGATGTTGTATCGGACA AGTCAGGCAAACGAGAGACAACAGTAGCATCTGACAGTGAAGAAGAAGCCGGGAAAGAAGAGTCTTCTGT GAAGAAGAAGGAAGACAAGGACCTGTTTGGGAGTGACAGTGAGTCCGGCAATGAAGAAGAAAATCTTATT GCAGATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACGGGTTTTAACCAAGAAGATTTGG AGGAAGAGAAAAACGAAACACAGCTAAAGGAAGCAGAAGATTCTGATTCTGATGATAACATAAAGAGAGG CAAGCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAAAGCATGTGTGGCAAACGC AGACGAAATCGAGATGGGGGCACTTTTATTAGTGATGCAGATGATGTTGTGAGCGCCATGATTGTCAAGA TGAATGAAGCTGCTGAGGTAAGCTCTTCTAGGTTACCCTTCTCCCCTCCTGCCCTCAACCAAATTGATGG GGTGGCAAATCTTTCTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC068184 |
| Insert Size | 1632 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068184, AAH68184 |
| RefSeq Size | 4353 bp |
| RefSeq ORF | 1631 bp |
| Locus ID | 73473 |
| Gene Summary | Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208664 | Iws1 (tGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 3420.00 |
|
| MR208664 | Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 4040.00 |
|
| MR208664L3 | Lenti ORF clone of Iws1 (Myc-DDK-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 5040.00 |
|
| MR208664L4 | Lenti ORF clone of Iws1 (mGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (cDNA clone MGC:93016 IMAGE:30639737) |
CNY 5040.00 |
