AAV Products and Services

What is AAV?

Adeno-associated virus (AAV) is a small, non-enveloped virus with a single stranded DNA genome. This virus is commonly used as an efficient vehicle to mediate DNA delivery into mammalian cells, both in vitro and in vivo.

Benefits of Using AAV

  • Tissue Specificity
  • High Transduction Efficiency
  • Long Term/Stable Gene Expression
  • Low Immunogenicity
  • Non-pathogenic
Figure 1: In vitro transduction efficiency of HEK293T cell line with different AAV serotypes.
AAV ORF®

Transduction-ready AAV Particles for Overexpression
AAV Control Particles

For Quantifying Transduction Efficiency
AAV Vectors

Flexible Reporter & Promoter Options
AAV Packaging Service

Multiple Serotypes Available
AAV Antibodies

Monoclonal & Polyclonal
AAV Serotyping Kit

For Optimizing your AAV Experiments

How does AAV gene delivery work?

AAV particles deliver your gene of interest and promote expression in a tissue-specific manner. Watch our webinar and download our free e-book below to learn more about AAV.

AAV

AAV Serotype Selection Guide

One of the useful features of AAV is its serotypes, which defines the preference of the types of cells/organs they infect. The tables below provide the selection guide for in vitro and in vivo applications.

Cell Line AAV-1 AAV-2 AAV-3 AAV-4 AAV-5 AAV-6 AAV-8 AAV-9 AAV-DJ AAV-DJ/8
Huh-7 13 100 2.5 0.0 0.1 10 0.7 0.0 500 0.2
HEK293 25 100 2.5 0.1 0.1 5 0.7 0.1 500 0.3
HeLa 3 100 2.0 0.1 6.7 1 0.2 0.1 667 0.2
HepG2 3 100 16.7 0.3 1.7 5 0.3 ND 1250 0.5
Hep1A 20 100 0.2 1.0 0.1 1 0.2 0.0 400 0.1
911 17 100 11 0.2 0.1 17 0.1 ND 500 0.0
CHO 100 100 14 1.4 333 50 10 1.0 25000 5.0
COS 33 100 33 3.3 5.0 14 2.0 0.5 500 0.3
MeWo 10 100 20 0.3 6.7 10 1.0 0.2 2857 1.0
NIH3T3 10 100 2.9 2.9 0.3 10 0.3 ND 500 0.1
A549 14 100 20 ND 0.5 10 0.5 0.1 1000 0.1
HT1180 20 100 10 0.1 0.3 33 0.5 0.1 333 0.2
Monocytes 1111 100 ND ND 125 1429 ND ND 100 ND
Immature DC 2500 100 ND ND 222 2857 ND ND 200 ND
Mature DC 2222 100 ND ND 333 3333 ND ND 100 ND

Note: Infectivity rates normalized to AAV-2 = 100. ND = Not Determined.

Grimm D, Lee JS, Wang L, et al. In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses. J Virol. 2008;82(12):5887-5911. doi:10.1128/JVI.00254-08

Issa, Shaza et al. Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells 2023, 12, 785. https://doi.org/10.3390/cells12050785

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