慢病毒载体已成为细胞中基因传递的主要工具，由人类免疫缺陷 I 型病毒（HIV-1）去除许多毒基因改造而得。慢病毒载体包含LTRs 和包装信号( Ψ ).病毒包装的基因由几个质粒共同提供,因此获得的假型病毒属于复制缺陷型。
- 广泛的转染细胞普系: 慢病毒可转染许多细胞，包括分裂的&非分裂的，易转染的&难转染的细胞。
- Lenti protocol
- Recommended lentivirus MOI for common cell lines
- Does lentivirus work with neurons?
- Frequently asked questions about lenti
- How to make lentivirus?
- Webinar: How to make lentivirus
- Lenti webinar
- Lenti biosafety
"I have used shLenti for knockdown (TXN1 and TXNRD1) and they have worked well."
"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals."
"Lenti Vector with GFP and selection marker makes easy to generate single cell clones."
">95% of the time we achieve expression which is better than my experience with other vendors."
"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."
"I choose OriGene's lentivirus to induce effectively long-term and deficiency of PTP1B in a primary cell line then transplanted into mice. With OriGene Lenti we achieved a downregulation of PTP1B sustained for more than 5 days, which allowed us to assess the effect of PTP1B absence in early stages of cells engraftment!"
"I have used lenti-ORF plasmids for the generation of overexpression stable cells. They work well. The cell selection marker in the vector made it easier to generate stable cells."
"The ready-to-use lentivirus particles worked well in our hard-to-transduce cell lines."
"I purchased a lenti-ORF from OriGene. I have good transfection and transduction efficiency with OriGene's lenti products. Overall, I am extremely satisfied."
"I used both the lentivirus and the lenti plasmids. Both of them worked very well. And the customer service is good too."
"We Purchased multiple lenti vectors from OriGene and they all worked great."
"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."