IFT140 (NM_014714) Human Untagged Clone
CAT＃: SC323979
IFT140 (untagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140)
CNY 10008.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c305C8.4; c380F5.1; gs114; MZSDS; RP80; SRTD9; WDTC2 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_014714.2
CCACGCGTCCGCAGAGGTTTCAGCGCTGAGGAGGCCTGAGTTCCGTCATGGCCCTCTATT
ATGACCACCAGATAGAAGCCCCGGATGCAGCAGGGTCACCCTCATTTATCAGCTGGCACC CTGTCCATCCATTCTTGGCAGTTGCTTACATCAGCACAACCTCAACAGGCAGCGTGGATA TTTACCTGGAGCAAGGGGAGTGCGTGCCAGATACACACGTCGAGAGGCCGTTCCGGGTTG CTTCCCTGTGCTGGCACCCGACGCGGCTGGTGCTGGCTGTGGGCTGGGAGACTGGAGAAG TGACGGTGTTTAACAAGCAGGACAAGGAGCAGCACACGATGCCCCTGACACACACAGCCG ACATCACCGTGCTCCGTTGGAGCCCCAGTGGAAACTGCCTGCTGTCTGGGGACAGGCTTG GTGTCTTGCTCTTGTGGAGGTTGGACCAAAGGGGCCGAGTGCAAGGGACGCCTCTGCTGA AACACGAGTATGGGAAACACCTCACGCACTGCATCTTCCGGCTCCCCCCTCCTGGCGAGG ACCTGGTTCAGTTGGCAAAGGCAGCTGTGAGCGGTGATGAGAAAGCCCTGGACATGTTTA ACTGGAAGAAGAGCAGTTCTGGAAGTTTGCTGAAGATGGGGTCTCACGAGGGGCTGTTGT TCTTTGTCAGTCTGATGGACGGGACAGTGCACTATGTGGATGAGAAGGGCAAGACCACTC AGGTGGTGTCCGCAGACAGCACGATTCAGATGCTGTTCTACATGGAGAAGAGGGAGGCAC TGGTGGTGGTCACAGAGAACCTCCGGCTGTCCCTGTACACGGTGCCTCCTGAGGGCAAAG CAGAAGAAGTGATGAAGGTCAAGCTGAGCGGGAAAACCGGCCGCCGGGCAGACATCGCTT TGATTGAAGGCAGCCTTCTCGTGATGGCCGTCGGGGAGGCTGCCCTCAGATTCTGGGACA TAGAACGAGGAGAGAATTATATACTGAGTCCAGATGAGAAGTTTGGCTTTGAGAAAGGAG AGAATATGAACTGTGTGTGTTACTGTAAAGTCAAAGGTCTTCTGGCCGCTGGTACCGACA GAGGGCGAGTAGCCATGTGGAGGAAAGTACCAGACTTCCTGGGCAGCCCCGGGGCAGAGG GCAAGGACAGGTGGGCCCTTCAGACCCCTACCGAGCTCCAAGGAAACATCACGCAAATCC AGTGGGGTTCCAGGAAGAACCTGCTGGCAGTGAACAGCGTCATCTCCGTGGCCATCCTCA GCGAGCGGGCCATGTCGTCACACTTCCACCAGCAAGTGGCCGCCATGCAGGTCTCCCCGA GTCTGCTGAATGTGTGCTTCCTGTCCACGGGGGTCGCACACAGCCTGCGCACCGACATGC ACATCAGTGGAGTGTTTGCCACCAAGGATGCTGTCGCAGTCTGGAACGGAAGGCAGGTGG CGATCTTCGAGCTTTCTGGAGCCGCGATACGGAGTGCAGGGACCTTCTTGTGTGAGACGC CTGTGTTAGCAATGCATGAAGAAAACGTTTACACGGTGGAGTCAAACCGAGTTCAAGTTC GAACCTGGCAGGGGACTGTCAAACAACTCCTCCTTTTCTCGGAGACTGAGGGGAATCCCT GCTTCTTGGACATCTGTGGGAATTTCCTGGTTGTAGGGACAGACTTGGCTCACTTTAAAA GCTTTGATCTTTCCCGAAGAGAGGCCAAAGCACACTGTAGCTGCAGGAGCCTGGCGGAGC TGGTCCCTGGGGTGGGGGGCATCGCTTCTCTGCGGTGCAGCAGCAGCGGGAGCACCATCA GCATCCTCCCCAGCAAGGCTGACAACAGCCCTGATTCCAAAATCTGCTTCTACGATGTTG AAATGGACACAGTGACCGTCTTTGACTTCAAGACTGGACAAATTGATCGGAGAGAGACGC TGTCCTTTAATGAGCAAGAGACTAATAAGAGCCACCTCTTTGTGGATGAGGGACTGAAAA ATTATGTTCCCGTGAACCACTTCTGGGACCAGAGTGAGCCCCGGCTGTTTGTATGCGAAG CCGTGCAGGAGACGCCGCGCTCCCAGCCTCAGTCTGCAAACGGGCAGCCCCAAGATGGGC GCGCTGGCCCTGCGGCAGATGTTTTGATCCTGTCCTTCTTCATTTCCGAAGAGCACGGCT TCCTGCTTCATGAGAGCTTCCCCCGGCCTGCCACCTCCCACAGTCTCCTGGGGATGGAAG TGCCTTATTACTACTTCACAAGAAAGCCCGAAGAAGCAGACAGAGAAGACGAGGTGGAGC CTGGGTGCCACCACATCCCTCAGATGGTGTCCAGGAGACCCCTGCGAGACTTTGTGGGGC TGGAGGACTGCGACAAGGCCACCCGGGACGCCATGCTCCACTTCAGCTTCTTTGTCACCA TAGGAGACATGGACGAAGCCTTCAAATCCATCAAGCTCATCAAAAGTGAGGCCGTCTGGG AGAACATGGCGCGCATGTGCGTGAAGACCCAGCGGCTGGACGTGGCCAAGGTGTGCCTGG GGAACATGGGCCATGCCCGCGGGGCCCGAGCGCTGCGTGAGGCGGAGCAGGAGCCGGAGC TAGAGGCCCGCGTGGCCGTGCTGGCCACGCAGCTGGGCATGCTGGAGGACGCCGAGCAGC TGTACAGGAAGTGCAAGCGCCACGACCTCCTGAACAAGTTCTACCAGGCTGCGGGCCGGT GGCAGGAGGCCCTCCAGGTAGCCGAGCACCACGATCGCGTGCACCTGCGCAGCACCTACC ACCGCTATGCCGGGCACCTGGAGGCCAGCGCCGACTGCAGCCGGGCCCTCAGTTACTACG AGAAGTCGGACACGCACCGCTTCGAGGTGCCCAGGATGCTGTCGGAGGACCTGCCGTCCC TGGAGCTCTACGTGAATAAAATGAAGGATAAGACCCTGTGGCGGTGGTGGGCGCAGTACC TGGAGAGCCAGGGCGAGATGGACGCCGCGCTGCACTACTACGAGCTGGCCCGGGACCACT TCTCCCTGGTCCGCATCCACTGCTTCCAGGGCAATGTCCAGAAGGCTGCGCAAATAGCCA ACGAGACAGGAAACCTGGCGGCCTCCTACCACCTCGCCCGCCAGTACGAGAGCCAGGAGG AGGTCGGGCAGGCGGTGCACTTCTACACCCGGGCACAGGCCTTCAAGAATGCCATCCGCC TGTGCAAGGAGAACGGCCTGGACGACCAGCTCATGAACTTGGCCCTGCTGAGCTCCCCCG AGGACATGATCGAGGCGGCCCGATACTACGAGGAGAAGGGCGTGCAGATGGACAGGGCGG TCATGCTGTACCACAAGGCTGGCCACTTCTCCAAGGCCCTGGAGCTGGCCTTTGCCACCC AGCAGTTTGTGGCCCTACAGCTCATAGCAGAGGACCTGGATGAGACGTCAGACCCTGCGC TCCTGGCCCGCTGCTCCGACTTCTTCATCGAGCACAGTCAGTACGAGAGGGCGGTAGAGC TGCTGCTGGCTGCCAGGAAGTATCAGGAAGCCCTGCAGCTGTGCCTGGGGCAGAACATGA GCATCACCGAGGAGATGGCGGAAAAGATGACCGTGGCCAAGGACTCCTCGGACCTGCCTG AGGAGTCGCGGCGGGAGCTGCTGGAGCAGATAGCAGACTGCTGCATGCGCCAGGGCAGCT ACCACCTGGCCACCAAGAAGTACACGCAGGCCGGCAACAAGCTGAAGGCCATGAGGGCGC TGCTCAAATCCGGAGACACGGAGAAAATCACGTTCTTCGCGAGCGTGTCCAGGCAGAAGG AAATCTACATCATGGCTGCTAACTACCTGCAGTCCCTGGACTGGCGGAAGGAGCCGGAGA TCATGAAGAACATCATCGGCTTCTACACCAAGGGGCGGGCCCTGGACCTCCTGGCTGGCT TTTATGACGCTTGTGCCCAGGTGGAGATTGATGAATACCAGAACTACGACAAAGCCCACG GGGCGCTGACCGAGGCCTACAAGTGCCTGGCCAAGGCCAAGGCCAAGAGCCCCCTGGACC AGGAGACCAGGCTGGCGCAGCTGCAGAGCAGGATGGCACTGGTGAAGAGGTTCATCCAGG CCCGCAGGACGTACACAGAGGACCCCAAGGAGTCCATCAAGCAGTGTGAGCTGCTCCTGG AGGAACCAGACCTGGACAGCACCATCCGCATCGGGGACGTCTATGGCTTCCTGGTGGAGC ACTACGTGCGGAAGGAGGAATACCAGACGGCCTACAGATTCCTGGAGGAGATGCGGCGGC GGCTTCCCTTGGCCAACATGTCCTACTACGTGAGCCCGCAGGCCGTGGACGCCGTGCACC GGGGGCTGGGTCTCCCACTGCCACGCACCGTCCCCGAGCAGGTCCGCCACAACAGCATGG AGGACGCCAGGGAGCTGGACGAGGAGGTGGTGGAAGAGGCAGATGACGACCCCTGAGGGG CCTGGGCCCCAGGACCAGCGTGCTGCTGCAGAAAGGCATCTTCTGGAATTTTTTTGTCAG CTGTGGCAAAGCCAGCATTTTTGCTGGGAAAAAACATGTCTGTGTTGGAATACGCGACAG AGCTGGGCGAGAACGCAGCGGCCCGGGCCGGCGGAGGGTGTGACCCGTCTGCACCTTGCT CTGTCCCACCTGCCTCTGGGTGCCCGGCAGCTCCACTAGATTTTTGGATTCATTCCTTTG AAGGGAGTCGGGTTCACCCTTCCATCGTATTCTCCCAACTACACATTGTAAAGCCTGAGA AACTTCTAGAACCTCAGGAAGCTGCAGCTGGAGGGCTGGGGCACCTGCCCCCCTGCTCCC CACACATCATATCCTCCCCATACTCCTGCAGGGCCCACGGCTCCTGAGCAACAGCTGGGA CACCCGGGCCTTGGCGGCTGCACCCCCTGCTAGGCTCTGCCCACCGGCCACCAACACTCC TGTAATTCCAATAAAGCAGTTTATTTTCTGAAAAAAAAAAAAAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_014714 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014714.2, NP_055529.2 |
| RefSeq Size | 4966 bp |
| RefSeq ORF | 4389 bp |
| Locus ID | 9742 |
| UniProt ID | Q96RY7 |
| Domains | WD40 |
| Gene Summary | This gene encodes one of the subunits of the intraflagellar transport (IFT) complex A. Intraflagellar transport is involved in the genesis, resorption and signaling of primary cilia. The primary cilium is a microtubule-based sensory organelle at the surface of most quiescent mammalian cells, that receives signals from its environment, such as the flow of fluid, light or odors, and transduces those signals to the nucleus. Loss of the corresponding protein in mouse results in renal cystic disease. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207528 | IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 10008.00 |
|
| RC207528L1 | Lenti-ORF clone of IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 12408.00 |
|
| RC207528L2 | Lenti-ORF clone of IFT140 (mGFP-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RC207528L3 | Lenti-ORF clone of IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RC207528L4 | Lenti-ORF clone of IFT140 (mGFP-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RG207528 | IFT140 (tGFP-tagged) - Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 11608.00 |
|
| SC108754 | IFT140 (untagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 10008.00 |
