IFT140 (NM_014714) Human Untagged Clone
CAT＃: SC108754
IFT140 (untagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140)
CNY 10008.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | c305C8.4; c380F5.1; gs114; MZSDS; RP80; SRTD9; WDTC2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108754 sequence for NM_014714 edited (data generated by NextGen Sequencing)
ATGGCCCTCTATTATGACCACCAGATAGAAGCCCCGGATGCAGCAGGGTCACCCTCATTT ATCAGCTGGCACCCTGTCCATCCATTCTTGGCAGTTGCTTACATCAGCACAACCTCAACA GGCAGCGTGGATATTTACCTGGAGCAAGGGGAGTGCGTGCCAGATACACACGTCGAGAGG CCGTTCCGGGTTGCTTCCCTGTGCTGGCACCCGACGCGGCTGGTGCTGGCTGTGGGCTGG GAGACTGGAGAAGTGACGGTGTTTAACAAGCAGGACAAGGAGCAGCACACGATGCCCCTG ACACACACAGCCGACATCACCGTGCTCCGTTGGAGCCCCAGTGGAAACTGCCTGCTGTCT GGGGACAGGCTTGGTGTCTTGCTCTTGTGGAGGTTGGACCAAAGGGGCCGAGTGCAAGGG ACGCCTCTGCTGAAACACGAGTATGGGAAACACCTCACGCACTGCATCTTCCGGCTCCCC CCTCCTGGCGAGGACCTGGTTCAGTTGGCAAAGGCAGCTGTGAGCGGTGATGAGAAAGCC CTGGACATGTTTAACTGGAAGAAGAGCAGTTCTGGAAGTTTGCTGAAGATGGGGTCTCAC GAGGGGCTGTTGTTCTTTGTCAGTCTGATGGACGGGACAGTGCACTATGTGGATGAGAAG GGCAAGACCACTCAGGTGGTGTCCGCAGACAGCACGATTCAGATGCTGTTCTACATGGAG AAGAGGGAGGCACTGGTGGTGGTCACAGAGAACCTCCGGCTGTCCCTGTACACGGTGCCT CCTGAGGGCAAAGCAGAAGAAGTGATGAAGGTCAAGCTGAGCGGGAAAACCGGCCCCCGG GCAGACATCGCTTTGATTGAAGGCAGCCTTCTCGTGATGGCCGTCGGGGAGGCTGCCCTC AGATTCTGGGACATAGAACGAGGAGAGAATTATATACTGAGTCCAGATGAGAAGTTTGGC TTTGAGAAAGGAGAGAATATGAACTGTGTGTGTTACTGTAAAGTCAAAGGTCTTCTGGCC GCTGGTACCGACAGAGGGCGAGTAGCCATGTGGAGGAAAGTACCAGACTTCCTGGGCAGC CCCGGGGCAGAGGGCAAGGACAGGTGGGCCCTTCAGACCCCTACCGAGCTCCAAGGAAAC ATCACGCAAATCCAGTGGGGTTCCAGGAAGAACCTGCTGGCAGTGAACAGCGTCATCTCC GTGGCCATCCTCAGCGAGCGGGCCATGTCGTCACACTTCCACCAGCAAGTGGCCGCCATG CAGGTCTCCCCGAGTCTGCTGAATGTGTGCTTCCTGTCCACGGGGGTCGCACACAGCCTG CGCACCGACATGCACATCAGTGGAGTGTTTGCCACCAAGGATGCTGTCGCAGTCTGGAAC GGAAGGCAGGTGGCGATCTTCGAGCTTTCTGGAGCCGCGATACGGAGTGCAGGGACCTTC TTGTGTGAGACGCCTGTGTTAGCAATGCATGAAGAAAACGTTTACACGGTGGAGTCAAAC CGAGTTCAAGTTCGAACCTGGCAGGGGACTGTCAAACAACTCCTCCTTTTCTCGGAGACT GAGGGGAATCCCTGCTTCTTGGACATCTGTGGGAATTTCCTGGTTGTAGGGACAGACTTG GCTCACTTTAAAAGCTTTGATCTTTCCCGAAGAGAGGCCAAAGCACACTGTAGCTGCAGG AGCCTGGCGGAGCTGGTCCCTGGGGTGGGGGGCATCGCTTCTCTGCGGTGCAGCAGCAGC GGGAGCACCATCAGCATCCTCCCCAGCAAGGCTGACAACAGCCCTGATTCCAAAATCTGC TTCTACGATGTTGAAATGGACACAGTGACCGTCTTTGACTTCAAGACTGGACAAATTGAT CGGAGAGAGACGCTGTCCTTTAATGAGCAAGAGACTAATAAGAGCCACCTCTTTGTGGAT GAGGGACTGAAAAATTATGTTCCCGTGAACCACTTCTGGGACCAGAGTGAGCCCCGGCTG TTTGTATGCGAAGCCGTGCAGGAGACGCCGCGCTCCCAGCCTCAGTCTGCAAACGGGCAG CCCCAAGATGGGCGCGCTGGCCCTGCGGCAGATGTTTTGATCCTGTCCTTCTTCATTTCC GAAGAGCACGGCTTCCTGCTTCATGAGAGCTTCCCCCGGCCTGCCACCTCCCACAGTCTC CTGGGGATGGAAGTGCCTTATTACTACTTCACAAGAAAGCCCGAAGAAGCAGACAGAGAA GACGAGGTGGAGCCTGGGTGCCACCACATCCCTCAGATGGTGTCCAGGAGACCCCTGCGA GACTTTGTGGGGCTGGAGGACTGCGACAAGGCCACCCGGGACGCCATGCTCCACTTCAGC TTCTTTGTCACCATAGGAGACATGGACGAAGCCTTCAAATCCATCAAGCTCATCAAAAGT GAGGCCGTCTGGGAGAACATGGCGCGCATGTGCGTGAAGACCCAGCGGCTGGACGTGGCC AAGGTGTGCCTGGGGAACATGGGCCATGCCCGCGGGGCCCGAGCGCTGCGTGAGGCGGAG CAGGAGCCGGAGCTAGAGGCCCGCGTGGCCGTGCTGGCCACGCAGCTGGGCATGCTGGAG GACGCCGAGCAGCTGTACAGGAAGTGCAAGCGCCACGACCTCCTGAACAAGTTCTACCAG GCTGCGGGCCGGTGGCAGGAGGCCCTCCAGGTAGCCGAGCACCACGATCGCGTGCACCTG CGCAGCACCTACCACCGCTATGCCGGGCACCTGGAGGCCAGCGCCGACTGCAGCCGGGCC CTCAGTTACTACGAGAAGTCGGACACGCACCGCTTCGAGGTGCCCAGGATGCTGTCGGAG GACCTGCCGTCCCTGGAGCTCTACGTGAATAAAATGAAGGATAAGACCCTGTGGCGGTGG TGGGCGCAGTACCTGGAGAGCCAGGGCGAGATGGACGCCGCGCTGCACTACTACGAGCTG GCCCGGGACCACTTCTCCCTGGTCCGCATCCACTGCTTCCAGGGCAATGTCCAGAAGGCT GCGCAAATAGCCAACGAGACAGGAAACCTGGCGGCCTCCTACCACCTCGCCCGCCAGTAC GAGAGCCAGGAGGAGGTCGGGCAGGCGGTGCACTTCTACACCCGGGCACAGGCCTTCAAG AATGCCATCCGCCTGTGCAAGGAGAACGGCCTGGACGACCAGCTCATGAACTTGGCCCTG CTGAGCTCCCCCGAGGACATGATCGAGGCGGCCCGATACTACGAGGAGAAGGGCGTGCAG ATGGACAGGGCGGTCATGCTGTACCACAAGGCTGGCCACTTCTCCAAGGCCCTGGAGCTG GCCTTTGCCACCCAGCAGTTTGTGGCCCTACAGCTCATAGCAGAGGACCTGGATGAGACG TCAGACCCTGCGCTCCTGGCCCGCTGCTCCGACTTCTTCATCGAGCACAGTCAGTACGAG AGGGCGGTAGAGCTGCTGCTGGCTGCCAGGAAGTATCAGGAAGCCCTGCAGCTGTGCCTG GGGCAGAACATGAGCATCACCGAGGAGATGGCGGAAAAGATGACCGTGGCCAAGGACTCC TCGGACCTGCCTGAGGAGTCGCGGCGGGAGCTGCTGGAGCAGATAGCAGACTGCTGCATG CGCCAGGGCAGCTACCACCTGGCCACCAAGAAGTACACGCAGGCCGGCAACAAGCTGAAG GCCATGAGGGCGCTGCTCAAATCCGGAGACACGGAGAAAATCACGTTCTTCGCGAGCGTG TCCAGGCAGAAGGAAATCTACATCATGGCTGCTAACTACCTGCAGTCCCTGGACTGGCGG AAGGAGCCGGAGATCATGAAGAACATCATCGGCTTCTACACCAAGGGGCGGGCCCTGGAC CTCCTGGCTGGCTTTTATGACGCTTGTGCCCAGGTGGAGATTGATGAATACCAGAACTAC GACAAAGCCCACGGGGCGCTGACCGAGGCCTACAAGTGCCTGGCCAAGGCCAAGGCCAAG AGCCCCCTGGACCAGGAGACCAGGCTGGCGCAGCTGCAGAGCAGGATGGCACTGGTGAAG AGGTTCATCCAGGCCCGCAGGACGTACACAGAGGACCCCAAGGAGTCCATCAAGCAGTGT GAGCTGCTCCTGGAGGAACCAGACCTGGACAGCACCATCCGCATCGGGGACGTCTATGGC TTCCTGGTGGAGCACTACGTGCGGAAGGAGGAATACCAGACGGCCTACAGATTCCTGGAG GAGATGCGGCGGCGGCTTCCCTTGGCCAACATGTCCTACTACGTGAGCCCGCAGGCCGTG GACGCCGTGCACCGGGGGCTGGGTCTCCCACTGCCACGCACCGTCCCCGAGCAGGTCCGC CACAACAGCATGGAGGACGCCAGGGAGCTGGACGAGGAGGTGGTGGAAGAGGCAGATGAC GACCCCTGA Clone variation with respect to NM_014714.3 836 g=>c >OriGene 5' read for NM_014714 unedited
TAACCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGTGGCCTTAGGGCTAGGGCGGCCGACCCAGGTCCTTAGGAGCCAG GCCGGGGTTTGGGGCCGAGGATGTGACCGGTGGCGGGCGTCGGGACCCTCCCGTCTCCTC CCACGATCCCGGCGCCGCCGAGTCCCCGCGGCCCCTCGCGAGTCCCATCGAGGCTGGGGC GGGCTTGGCGCGGGGTCCAGGTCGCTCGTCGGACTATGGCTGTCGCCCCCGCCCGGCCTC GAATCGCGGCTGATTCCGGTTTCAGCGCTGAGGAGGCCTGAGTTCCGTCATGGCCCTCTA TTATGACCACCAGATAGAAGCCCCGGATGCAGCAGGGTCACCCTCATTTATCAGCTGGCA CCCTGTCCATCCATTCTTGGCAGTTGCTTACATCAGCACAACCTCAACAGGCAGCGTGGA TATTTACCTGGAGCAAGGGGAGTGCGTGCCAGATACACACGTCGAGAGGCCGTTCCGGGT TGCTTCCCTGTGCTGGCACCCGACGCGGCTGGTGCTGGCTGTGGGCTGGGAGACTGGAGA AGTGACGGTGTTTAACAAGCAGGACAAGGAGCAGCACACGATGCCCCTGACACACACAGC CGACATCACCGTGCTCCGTTGGAGCCCCAGTGGAAACTGCCTGCTGTCTGGNGACAGGCT GGNTGTCCTGCTCTTGTGGAGGNTGGACCANAGGGCCGAGTGCAAGGGACGCCTCTGCTG AACACCGAGTATGGGAACACCTCACGCACTGCATCTTCCGGCTCCCCCTCTGGCAGGACT CGGGTTCANTTGGCAAGGCAGCTGTGAGCCGTGATGAAAAGCCTGGNATGTTAACTGAGA AAGAGCAGTT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_014714 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014714.2, NP_055529.2 |
| RefSeq Size | 4966 bp |
| RefSeq ORF | 4389 bp |
| Locus ID | 9742 |
| UniProt ID | Q96RY7 |
| Domains | WD40 |
| Gene Summary | This gene encodes one of the subunits of the intraflagellar transport (IFT) complex A. Intraflagellar transport is involved in the genesis, resorption and signaling of primary cilia. The primary cilium is a microtubule-based sensory organelle at the surface of most quiescent mammalian cells, that receives signals from its environment, such as the flow of fluid, light or odors, and transduces those signals to the nucleus. Loss of the corresponding protein in mouse results in renal cystic disease. [provided by RefSeq, Jun 2012] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207528 | IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 10008.00 |
|
| RC207528L1 | Lenti-ORF clone of IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 12408.00 |
|
| RC207528L2 | Lenti-ORF clone of IFT140 (mGFP-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RC207528L3 | Lenti-ORF clone of IFT140 (Myc-DDK-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RC207528L4 | Lenti-ORF clone of IFT140 (mGFP-tagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 16530.00 |
|
| RG207528 | IFT140 (tGFP-tagged) - Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 11608.00 |
|
| SC323979 | IFT140 (untagged)-Human intraflagellar transport 140 homolog (Chlamydomonas) (IFT140) |
CNY 10008.00 |
