TFIIE alpha (GTF2E1) (NM_005513) Human Mass Spec Standard

Specifications

Product Data
Description	GTF2E1 MS Standard C13 and N15-labeled recombinant protein (NP_005504)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC218763
Predicted MW	49.3 kDa
Protein Sequence	Sequence Link (showhide) >RC218763 representing NM_005513 Red=Cloning site Green=Tags(s) MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDK FIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLE ANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTE IPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIW LRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAAN GDDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTP EEKEAYIAMGQRMFEDLFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_005504
RefSeq Size	2969
RefSeq ORF	1317
Synonyms	FE; TF2E1; TFIIE-A
Locus ID	2960
Cytogenetics	3q13.33
Summary	Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors

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