NCE2 (UBE2F) (NM_080678) Human Mass Spec Standard

Specifications

Product Data
Description	UBE2F MS Standard C13 and N15-labeled recombinant protein (NP_542409)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC203942
Predicted MW	20.9 kDa
Protein Sequence	Sequence Link (showhide) >RC203942 representing NM_080678 Red=Cloning site Green=Tags(s) MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVT PDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVV WGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_542409
RefSeq Size	1366
RefSeq ORF	555
Synonyms	NCE2
Locus ID	140739
Cytogenetics	2q37.3
Summary	Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]
Protein Pathways	Ubiquitin mediated proteolysis

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