DAP13 (NDUFA12) (NM_018838) Human Mass Spec Standard

Specifications

Product Data
Description	NDUFA12 MS Standard C13 and N15-labeled recombinant protein (NP_061326)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC203265
Predicted MW	17.1 kDa
Protein Sequence	Sequence Link (showhide) >RC203265 protein sequence Red=Cloning site Green=Tags(s) MELVQVLKRGLQQITGHGGLRGYLRVFFRTNDAKVGTLVGEDKYGNKYYEDNKQFFGRHRWVVYTTEMNG KNTFWDVDGSMVPPEWHRWLHSMTDDPPTTKPLAARKFIWTNHKFNVTGTPEQYVPYSTTRKKIQEWIPP STPYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_061326
RefSeq Size	592
RefSeq ORF	435
Synonyms	B17.2; DAP13; MC1DN23
Locus ID	55967
Cytogenetics	12q22
Summary	This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

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