SF3B5 (NM_031287) Human Mass Spec Standard

Specifications

Product Data
Description	SF3B5 MS Standard C13 and N15-labeled recombinant protein (NP_112577)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200977
Predicted MW	10.1 kDa
Protein Sequence	Sequence Link (showhide) >RC200977 protein sequence Red=Cloning site Green=Tags(s) MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLME KMLQPCGPPADKPEEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_112577
RefSeq Size	786
RefSeq ORF	258
Synonyms	SF3b10; Ysf3
Locus ID	83443
Cytogenetics	6q24.2
Summary	Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).[UniProtKB/Swiss-Prot Function]
Protein Pathways	Spliceosome

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