DHFR (NM_000791) Human Mass Spec Standard

Specifications

Product Data
Description	DHFR MS Standard C13 and N15-labeled recombinant protein (NP_000782)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200089
Predicted MW	21.5 kDa
Protein Sequence	Sequence Link (showhide) >RC200089 protein sequence Red=Cloning site Green=Tags(s) MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKG RINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIM QDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_000782
RefSeq Size	3932
RefSeq ORF	561
Synonyms	DHFRP1; DYR
Locus ID	1719
Cytogenetics	5q14.1
Summary	Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Folate biosynthesis, Metabolic pathways, One carbon pool by folate

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