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DHFR (NM_000791) Human Tagged ORF Clone
CAT＃: RC200089
DHFR (Myc-DDK-tagged)-Human dihydrofolate reductase (DHFR)
ORF Plasmid: tGFP
"NM_000791" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DHFRP1; DYR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200089 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGACCTGC CCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTCTTCAGTAGAAGGTAA ACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGGT AGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTC TAGATGATGCCTTAAAACTTACTGAACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGG TGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATG CAAGACTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGAATACC CAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGTATATGAGAAGAATGA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200089 protein sequence
Red=Cloning site Green=Tags(s) MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKG RINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIM QDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000791 |
| ORF Size | 561 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000791.4 |
| RefSeq Size | 3932 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 1719 |
| UniProt ID | P00374 |
| Domains | DiHfolate_red |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Folate biosynthesis, Metabolic pathways, One carbon pool by folate |
| MW | 21.5 kDa |
| Gene Summary | Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
DHFR/TYMS are positive regulators of glioma cell growth and modulate chemo-sensitivity to temozolomide
,Zhao, M;Tan, B;Dai, X;Shao, Y;He, Q;Yang, B;Wang, J;Weng, Q;,
Eur. J. Pharmacol.
,PubMed ID 31542479
[DHFR]
|
|
NADPH production by the oxidative pentose-phosphate pathway supports folate metabolism
,Chen, L;Zhang, Z;Hoshino, A;Zheng, HD;Morley, M;Arany, Z;Rabinowitz, JD;,
Nat Metab
,PubMed ID 31058257
[DHFR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200089L1 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200089L2 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged |
CNY 4,800.00 |
|
| RC200089L3 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200089L4 | Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged |
CNY 5,890.00 |
|
| RG200089 | DHFR (tGFP-tagged) - Human dihydrofolate reductase (DHFR) |
CNY 4,000.00 |
|
| SC321334 | DHFR (untagged)-Human dihydrofolate reductase (DHFR) |
CNY 2,400.00 |
