putative NS5A protein (NC_001655) Virus Tagged ORF Clone
CAT＃: VC102239
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for putative NS5A protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757360
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CNY 4940.00
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CNY 600.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102239 represents NCBI reference of NP_757360 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTCATAGCCTGGGGGCTCGAGATTTGGCAGTATGTGTGCAACTTCTTCGTCATCTGCTTTAACG TGCTCAAAGCAGGCGTCCAATCAATGGTGAACATCCCCGGATGTCCCTTTTACTCCTGTCAGAAAGGTTA CAAAGGACCCTGGATTGGTTCCGGTATGCTGCAGGCTCGGTGCCCGTGTGGCGCTGAGTTGATTTTTTCC GTGGAGAACGGGTTCGCCAAGCTTTATAAGGGACCAAGGACCTGTTCAAACTATTGGAGAGGGGCAGTGC CCGTGAACGCTCGGCTTTGTGGTAGTGCACGACCGGATCCTACCGATTGGACTAGTTTGGTGGTCAACTA TGGTGTTCGGGACTATTGCAAATATGAGAAAATGGGGGACCACATATTCGTGACAGCAGTGTCCAGTCCT AACGTCTGCTTCACCCAGGTGCCGCCCACCCTTAGAGCTGCCGTCGCAGTAGATGGAGTACAGGTGCAGT GCTACCTGGGCGAGCCCAAAACTCCTTGGACCACTAGCGCGTGTTGCTATGGACCAGATGGCAAGGGGAA GACCGTGAAGCTGCCCTTTCGAGTAGATGGCCACACACCCGGCGTCCGAATGCAGTTGAACCTGAGGGAT GCCCTGGAAACTAACGACTGTAACAGCACCAATAACACCCCAAGCGACGAAGCCGCAGTTAGTGCCCTGG TGTTTAAACAGGAGCTCCGACGGACCAATCAGCTGCTTGAGGCCATAAGTGCAGGCGTCGACACGACCAA ACTTCCCGCTCCCTCCATTGAAGAGGTGGTGGTGCGCAAAAGGCAGTTTCGCGCCAGAACAGGTTCCCTG ACACTGCCACCACCACCAAGGTCCGTTCCAGGCGTGTCTTGCCCCGAGTCACTTCAGCGGAGCGACCCTC TGGAGGGTCCTAGTAATCTTCCTCCCTCTCCACCTGTCCTGCAATTGGCCATGCCTATGCCCCTCCTGGG AGCAGGAGAGTGCAACCCCTTTACGGCCATTGGTTGCGCTATGACCGAGACAGGCGGCGGACCAGATGAC CTCCCCAGTTACCCTCCCAAGAAAGAAGTCTCTGAATGGTCAGACGAATCTTGGAGCACGGCCACTACGG CGAGCTCTTATGTGACTGGACCACCGTATCCCAAGATCCGGGGGAAGGACTCAACACAGTCAGCGCCCGC AAAGCGCCCCACTAAAAAAAAACTGGGAAAATCCGAATTTAGTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102239 representing NP_757360
Red=Cloning sites Green=Tags MGLIAWGLEIWQYVCNFFVICFNVLKAGVQSMVNIPGCPFYSCQKGYKGPWIGSGMLQARCPCGAELIFS VENGFAKLYKGPRTCSNYWRGAVPVNARLCGSARPDPTDWTSLVVNYGVRDYCKYEKMGDHIFVTAVSSP NVCFTQVPPTLRAAVAVDGVQVQCYLGEPKTPWTTSACCYGPDGKGKTVKLPFRVDGHTPGVRMQLNLRD ALETNDCNSTNNTPSDEAAVSALVFKQELRRTNQLLEAISAGVDTTKLPAPSIEEVVVRKRQFRARTGSL TLPPPPRSVPGVSCPESLQRSDPLEGPSNLPPSPPVLQLAMPMPLLGAGECNPFTAIGCAMTETGGGPDD LPSYPPKKEVSEWSDESWSTATTASSYVTGPPYPKIRGKDSTQSAPAKRPTKKKLGKSEFSC TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001655 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001655.1, NP_757360 |
| RefSeq ORF | 1236 bp |
| MW | 44.6 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102231 | Myc-DDK-tagged ORF clone of viral ORF for putative core protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757352 |
CNY 3800.00 |
|
| VC102232 | Myc-DDK-tagged ORF clone of viral ORF for putative E1 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757353 |
CNY 3800.00 |
|
| VC102233 | Myc-DDK-tagged ORF clone of viral ORF for putative E2 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757354 |
CNY 3800.00 |
|
| VC102234 | Myc-DDK-tagged ORF clone of viral ORF for putative protein p7 [Hepatitis GB virus B], codon optimized for human cell expression, NP_757355 |
CNY 3800.00 |
|
| VC102235 | Myc-DDK-tagged ORF clone of viral ORF for putative NS2 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757356 |
CNY 3800.00 |
|
| VC102236 | Myc-DDK-tagged ORF clone of viral ORF for putative NS3 proteinase/ATPase/helicase [Hepatitis GB virus B], codon optimized for human cell expression, NP_757357 |
CNY 7410.00 |
|
| VC102237 | Myc-DDK-tagged ORF clone of viral ORF for putative NS4A protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757358 |
CNY 3800.00 |
|
| VC102238 | Myc-DDK-tagged ORF clone of viral ORF for putative NS4B protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757359 |
CNY 3800.00 |
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