putative NS3 proteinase/ATPase/helicase (NC_001655) Virus Tagged ORF Clone

CAT＃: VC102236

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for putative NS3 proteinase/ATPase/helicase [Hepatitis GB virus B], codon optimized for human cell expression, NP_757357

Datasheet

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CNY 7410.00

货期*

4周

规格

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102236 represents NCBI reference of NP_757357 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCTTCACACTGCAATGTTTGTCCGAACGGGGTACCCTGTCCGCCATGGCCGTGGTGATGACCG GAATAGACCCTCGGACTTGGACTGGGACTATCTTCAGACTTGGAAGCTTGGCCACCTCCTATATGGGTTT CGTTTGCGACAATGTACTGTATACCGCCCATCACGGGTCAAAGGGACGGCGCTTGGCACACCCTACAGGC TCCATCCACCCCATCACCGTTGACGCTGCCAACGATCAAGACATCTATCAACCTCCTTGTGGAGCCGGCA GTCTGACACGGTGCAGCTGTGGGGAAACTAAGGGATACCTCGTTACTAGGCTTGGGTCACTCGTCGAAGT GAATAAGAGCGACGATCCTTACTGGTGCGTGTGCGGCGCTCTGCCCATGGCCGTCGCAAAGGGGAGCAGT GGGGCTCCGATCCTGTGTTCATCTGGCCATGTGATTGGCATGTTCACTGCTGCTCGAAACAGTGGAGGAA GCGTGTCTCAGATCAGAGTCCGGCCTCTGGTTTGCGCTGGCTACCACCCCCAGTATACGGCCCATGCTAC CCTGGACACTAAGCCAACGGTGCCAAATGAGTACTCTGTCCAGATACTGATTGCCCCTACTGGGTCAGGC AAGTCCACTAAGCTGCCATTGTCCTATATGCAGGAAAAATATGAAGTGCTGGTCCTGAACCCCAGTGTGG CCACCACCGCATCTATGCCAAAGTATATGCATGCTACTTATGGCGTTAACCCTAATTGCTACTTTAACGG AAAATGCACTAACACGGGGGCTAGCCTTACATATTCCACATACGGCATGTATTTGACCGGTGCATGTAGC AGAAACTACGATGTTATCATTTGCGACGAGTGCCACGCCACCGACGCAACTACGGTCCTCGGGATCGGAA AGGTGCTCACAGAGGCCCCTTCCAAGAATGTGCGACTGGTCGTGCTCGCAACTGCCACCCCCCCGGGTGT GATCCCAACCCCTCATGCTAATATTACCGAAATCCAACTGACAGACGAAGGGACCATTCCCTTCCACGGG AAAAAGATAAAGGAAGAAAATCTGAAGAAAGGCCGGCACTTGATATTCGAGGCTACTAAAAAGCATTGCG ATGAACTGGCCAATGAGCTGGCAAGGAAGGGGATCACTGCCGTCTCCTATTACCGGGGCTGCGACATCAG TAAAATCCCTGAGGGGGACTGCGTCGTAGTTGCCACCGACGCCCTGTGTACAGGTTATACTGGCGACTTT GACAGTGTCTATGATTGCAGCCTCATGGTGGAGGGTACCTGTCACGTCGACCTTGACCCAACTTTCACCA TGGGAGTTCGGGTGTGCGGAGTATCAGCCATTGTGAAAGGGCAGCGCCGGGGAAGAACAGGGAGGGGGCG AGCCGGGATTTACTATTACGTGGACGGCTCATGTACCCCTAGCGGCATGGTGCCTGAATGTAACATCGTG GAAGCATTTGACGCAGCGAAAGCTTGGTATGGCCTGAGTAGTACAGAGGCCCAGACAATCCTCGATACAT ACAGAACACAACCTGGGCTTCCTGCCATTGGGGCTAACCTCGACGAATGGGCGGATTTGTTCTCTATGGT TAACCCAGAGCCCTCCTTTGTTAATACCGCTAAGAGAACGGCTGACAATTACGTTCTGCTGACAGCAGCC CAGCTGCAGCTTTGTCATCAGTATGGCTACGCGGCCCCCAATGACGCCCCCAGATGGCAGGGCGCTCGCC TCGGCAAAAAACCATGTGGGGTGCTGTGGAGGCTCGACGGAGCAGACGCCTGCCCGGGACCAGAGCCCAG CGAGGTGACTAGATATCAGATGTGCTTTACAGAAGTGAACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102236 representing NP_757357 Red=Cloning sites Green=Tags MAPFTLQCLSERGTLSAMAVVMTGIDPRTWTGTIFRLGSLATSYMGFVCDNVLYTAHHGSKGRRLAHPTG SIHPITVDAANDQDIYQPPCGAGSLTRCSCGETKGYLVTRLGSLVEVNKSDDPYWCVCGALPMAVAKGSS GAPILCSSGHVIGMFTAARNSGGSVSQIRVRPLVCAGYHPQYTAHATLDTKPTVPNEYSVQILIAPTGSG KSTKLPLSYMQEKYEVLVLNPSVATTASMPKYMHATYGVNPNCYFNGKCTNTGASLTYSTYGMYLTGACS RNYDVIICDECHATDATTVLGIGKVLTEAPSKNVRLVVLATATPPGVIPTPHANITEIQLTDEGTIPFHG KKIKEENLKKGRHLIFEATKKHCDELANELARKGITAVSYYRGCDISKIPEGDCVVVATDALCTGYTGDF DSVYDCSLMVEGTCHVDLDPTFTMGVRVCGVSAIVKGQRRGRTGRGRAGIYYYVDGSCTPSGMVPECNIV EAFDAAKAWYGLSSTEAQTILDTYRTQPGLPAIGANLDEWADLFSMVNPEPSFVNTAKRTADNYVLLTAA QLQLCHQYGYAAPNDAPRWQGARLGKKPCGVLWRLDGADACPGPEPSEVTRYQMCFTEVNT TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001655
ORF Size	1863 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001655.1, NP_757357
RefSeq ORF	1863 bp
MW	66.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102231	Myc-DDK-tagged ORF clone of viral ORF for putative core protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757352	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102232	Myc-DDK-tagged ORF clone of viral ORF for putative E1 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757353	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102233	Myc-DDK-tagged ORF clone of viral ORF for putative E2 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757354	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102234	Myc-DDK-tagged ORF clone of viral ORF for putative protein p7 [Hepatitis GB virus B], codon optimized for human cell expression, NP_757355	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102235	Myc-DDK-tagged ORF clone of viral ORF for putative NS2 protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757356	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102237	Myc-DDK-tagged ORF clone of viral ORF for putative NS4A protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757358	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102238	Myc-DDK-tagged ORF clone of viral ORF for putative NS4B protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757359	10 ug 产品选项验证失败所有选项都是必需的	CNY 3800.00
VC102239	Myc-DDK-tagged ORF clone of viral ORF for putative NS5A protein [Hepatitis GB virus B], codon optimized for human cell expression, NP_757360	10 ug 产品选项验证失败所有选项都是必需的	CNY 4940.00

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