VPS39 (NM_001301138) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hVam6p; TLP; VAM6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301138, the custom clone sequence may differ by one or more nucleotides ATGCACGACGCTTTCGAGCCAGTGCCGATCCTAGAAAAGCTGCCTCTGCAAATCGACTGTCTGGCTGCCT GGGAGGAATGGCTTCTTGTGGGAACCAAACAAGGACATCTTCTTCTCTATAGGATTCGGAAGGACGTTGT GCCAGCAGATGTAGCATCACCTGAAAGCGGCAGTTGCAACAGATTTGAAGTGACACTAGAGAAATCCAAT AAGAACTTCTCCAAAAAGATTCAGCAGATCCATGTGGTTTCCCAGTTTAAGATTCTGGTCAGCTTGTTAG AAAATAACATTTATGTCCATGACCTATTGACATTTCAACAAATCACTACGGTTTCAAAGGCAAAGGGAGC ATCACTGTTTACTTGTGACCTCCAGCACACAGAGACCGGTGAGGAGGTGTTACGGATGTGTGTGGCAGTA AAAAAGAAGCTGCAGCTCTATTTCTGGAAGGACAGGGAATTTCATGAATTGCAGGGGGACTTTAGTGTGC CAGATGTGCCCAAGTCCATGGCGTGGTGTGAAAATTCTATCTGTGTGGGTTTCAAGAGAGACTACTACCT AATAAGGGTGGATGGAAAGGGGTCCATCAAAGAGCTCTTTCCAACAGGAAAACAGCTGGAGCCCTTAGTT GCACCTCTGGCAGATGGAAAAGTGGCTGTGGGCCAGGATGATCTCACCGTGGTACTCAATGAGGAAGGGA TCTGCACACAGAAATGTGCCCTGAACTGGACGGACATACCAGTGGCCATGGAGCACCAGCCTCCCTACAT CATTGCAGTGTTGCCTCGATATGTTGAGATCCGAACATTTGAACCGAGGCTTCTGGTCCAAAGCATTGAA TTGCAAAGGCCCCGTTTCATTACCTCAGGAGGATCAAACATTATCTATGTGGCCAGCAATCATTTTGTTT GGAGACTCATCCCTGTCCCCATGGCAACCCAAATCCAACAACTTCTCCAGGACAAGCAGTTTGAATTGGC TCTGCAGCTCGCAGAAATGAAAGATGATTCTGACAGTGAAAAGCAGCAACAAATTCATCACATCAAGAAC TTGTATGCCTTCAACCTCTTCTGCCAGAAGCGTTTTGATGAGTCCATGCAGGTCTTTGCTAAACTTGGCA CAGATCCCACCCATGTGATGGGCCTGTACCCTGACCTGCTGCCCACAGACTACAGAAAGCAGTTGCAGTA TCCCAACCCATTGCCTGTGCTCTCCGGGGCTGAATTGGAGAAGGCTCACTTAGCTCTGATTGACTACCTG ACACAGAAACGAAGTCAATTGGTAAAGAAGCTGAATGACTCTGATCACCAGTCAAGCACCTCACCGCTCA TGGAAGGCACTCCCACCATCAAATCCAAGAAGAAGCTGCTACAAATCATCGACACCACCCTGCTCAAGTG CTATCTCCATACAAATGTGGCCCTGGTGGCCCCCTTGCTACGCCTGGAGAACAATCACTGCCACATCGAG GAGAGCGAGCACGTGCTAAAGAAGGCTCACAAGTACAGTGAGCTTATCATCCTGTATGAGAAGAAGGGGC TCCACGAGAAAGCTCTGCAGGTGCTCGTGGACCAGTCCAAGAAAGCCAACTCCCCTCTGAAAGGCCACGA GAGGACAGTGCAGTATCTGCAGCATCTGGGCACAGAAAACCTGCATTTGATTTTCTCCTACTCAGTGTGG GTGCTGAGAGACTTCCCAGAAGATGGCCTGAAGATATTTACTGAAGATCTCCCGGAAGTGGAGTCTCTGC CACGTGATCGAGTCCTCGGCTTCTTAATAGAGAATTTTAAGGGTCTGGCTATTCCTTATCTGGAACACAT CATCCATGTTTGGGAGGAGACAGGCTCTCGGTTCCACAACTGCCTGATCCAGCTATACTGTGAGAAGGTG CAAGGTCTGATGAAGGAGTATCTCCTGTCCTTCCCTGCAGGCAAAACCCCAGTCCCAGCTGGAGAGGAAG AGGGTGAGCTGGGAGAATACCGGCAAAAGCTCCTCATGTTCTTGGAGATTTCCAGCTACTATGATCCAGG CCGGCTCATCTGTGATTTTCCCTTTGATGGCCTCTTAGAAGAACGAGCTCTCCTGTTGGGGCGCATGGGG AAACATGAACAAGCTCTTTTCATTTATGTCCACATCTTGAAGGATACAAGGATGGCTGAGGAGTACTGCC ACAAACACTATGACCGAAACAAAGATGGCAACAAAGATGTGTATCTGTCCCTGCTTCGGATGTACCTGTC GCCCCCCAGCATTCACTGCCTGGGGCCAATCAAGCTGGAACTACTGGAGCCAAAAGCCAACCTCCAGGCC GCTCTGCAGGTCCTCGAGCTACACCACAGCAAACTGGACACCACCAAGGCCCTCAACCTTCTGCCAGCAA ACACTCAGATCAATGACATACGCATCTTCCTGGAAAAGGTCTTGGAAGAAAATGCACAAAAGAAACGGTT CAATCAAGTGCTCAAGAACCTTCTCCATGCAGAATTCCTGAGGGTCCAGGAAGAGCGGATTTTACACCAG CAGGTGAAGTGCATCATCACAGAGGAGAAGGTGTGCATGGTGTGTAAGAAGAAGATTGGGAACAGTGCAT TTGCAAGATACCCCAATGGAGTGGTCGTCCATTACTTCTGTTCCAAAGAGGTAAACCCAGCTGACACTTG A
Restriction Sites	SgfI-MluI
ACCN	NM_001301138
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301138.1, NP_001288067.1
RefSeq Size	4906 bp
RefSeq ORF	2661 bp
Locus ID	23339
UniProt ID	Q96JC1
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that may promote clustering and fusion of late endosomes and lysosomes. The protein may also act as an adaptor protein that modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

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