SLC17A4 (NM_001286121) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KAIA2138
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336156 representing NM_001286121. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACTTGAGCATTGCCATCCCAGCTATGGTGAACAACACAGCCCCACCTAGCCAGCCCAATGCTTCC ACAGAACGGCCCTCCACTGACTCCCAGGGCTACTGGAATGAAACTCTAAAAGAATTTAAAGCAATGGCC CCTGCATATGACTGGAGTCCTGAAATCCAGGGAATCATCCTCAGCTCCCTCAACTATGGCTCATTCTTG GCTCCAATCCCCAGTGGCTATGTGGCTGGAATATTTGGAGCCAAGTATGTGGTTGGTGCTGGCTTGTTT ATTTCCTCATTCCTGACCCTCTTCATTCCACTGGCAGCTAATGCGGGAGTGGCCTTGCTCATTGTCCTC CGGATTGTACAAGGCATAGCCCAGGTTATGGTATTAACTGGTCAGTATTCAATTTGGGTCAAATGGGCT CCCCCACTGGAAAGGAGTCAACTCACCACCATTGCTGGATCAGGGTCAATGCTGGGGTCCTTCATTGTT CTACTTGCTGGTGGTCTCCTCTGCCAGACCATAGGATGGCCTTACGTCTTCTATATCTTTGGAGGAATT GGCTGTGCTTGTTGTCCTCTCTGGTTTCCTCTCATTTATGATGATCCTGTGAATCATCCCTTTATCAGT GCTGGTGAGAAGAGATACATTGTGTGTTCATTGGCTCAGCAGGACTGTTCACCAGGCTGGTCTCTTCCC ATTAGGGCTATGATCAAATCCTTACCACTCTGGGCCATTTTAGTCTCTTATTTCTGTGAATACTGGCTT TTTTATACCATTATGGCGTACACACCAACGTACATCAGCTCGGTACTTCAAGCCAACCTCAGAGATAGT GGGATCCTGTCTGCCTTGCCGTTTGTTGTTGGATGTATCTGCATTATCCTTGGAGGTCTACTGGCAGAC TTTCTTCTCTCCAGAAAAATCCTCAGACTCATCACCATCAGGAAACTCTTCACTGCCATTGGGGTTCTC TTCCCATCCGTGATCCTCGTGTCCCTGCCCTGGGTCAGATCCAGCCACAGCATGACCATGACCTTCTTG GTGCTGTCTTCTGCCATCAGCAGCTTCTGTGAATCAGGAGCCCTTGTTAACTTCTTGGATATTGCTCCT CGGTACACTGGCTTTCTCAAAGGACTATTGCAAGTCTTTGCACACATAGCTGGAGCCATCTCTCCTACT GCTGCTGGATTTTTCATCAGTCAGGATTCAGAGTTTGGTTGGAGAAATGTCTTCTTGCTTTCAGCTGCT GTTAACATATCGGGCCTGGTTTTCTACCTCATCTTTGGCCGAGCAGATGTGCAGGACTGGGCTAAAGAG CAGACATTCACCCACCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286121
Insert Size	1332 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286121.1
RefSeq Size	3716 bp
RefSeq ORF	1332 bp
Locus ID	10050
UniProt ID	Q9Y2C5
Protein Families	Transmembrane
MW	48.3 kDa
Gene Summary	Phosphate homeostasis is maintained by regulating intake, intestinal absorption, bone deposition and resorption, and renal excretion of phosphate. The central molecule in the control of phosphate excretion from the kidney is the sodium/phosphate cotransporter NPT1 (SLC17A1; MIM 182308), which is located in the renal proximal tubule. NPT1 uses the transmembrane electrochemical potential gradient of sodium to transport phosphate across the cell membrane. SLC17A4 is a similar sodium/phosphate cotransporter in the intestinal mucosa that plays an important role in the absorption of phosphate from the intestine (summary by Shibui et al., 1999 [PubMed 10319585]).[supplied by OMIM, Feb 2011] Transcript Variant: This variant (2) differs in its 5' UTR and initiaties translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

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