Rabex5 (RABGEF1) (NM_001287060) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	rabex-5; RABEX5; RAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335182 representing NM_001287060. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAACTCGTGGGAAAGTGCCTCCAGAAAGAGTCGAGAAGATAATGGATCAGATTGAAAAGTACATC ATGACTCGTCTCTATAAATATGTATTCTGTCCAGAAACTACTGATGATGAGAAGAAAGATCTTGCCATT CAAAAGAGAATCAGAGCCCTGCGCTGGGTTACGCCTCAGATGCTGTGTGTCCCTGTTAATGAAGACATC CCAGAAGTGTCTGATATGGTGGTGAAGGCGATCACAGATATCATTGAAATGGATTCCAAGCGTGTGCCT CGAGACAAGCTGGCCTGCATCACCAAGTGCAGCAAGCACATCTTCAATGCCATCAAGATCACCAAGAAT GAGCCGGCGTCAGCGGATGACTTCCTCCCCACCCTCATCTACATTGTTTTGAAGGGCAACCCCCCACGC CTTCAGTCTAATATCCAGTATATCACGCGCTTCTGCAATCCAAGCCGACTGATGACTGGAGAGGATGGC TACTATTTCACCAATCTGTGCTGTGCTGTGGCTTTCATTGAGAAGCTAGACGCCCAGTCTTTGAATCTA AGTCAGGAGGATTTTGATCGCTACATGTCTGGCCAGACCTCTCCCAGGAAGCAAGAAGCTGAGAGTTGG TCTCCTGATGCTTGCTTAGGCGTCAAGCAAATGTATAAGAACTTGGATCTCTTGTCTCAGTTGAATGAA CGACAAGAAAGGATCATGAATGAAGCCAAGAAACTGGAAAAAGACCTCATAGATTGGACAGATGGAATT GCAAGAGAAGTTCAAGACATCGTTGAGAAATACCCACTGGAAATTAAGCCTCCGAATCAACCGTTAGCA GCTATTGACTCTGAAAACGTTGAAAATGATAAACTTCCTCCACCACTGCAACCTCAAGTTTATGCAGGA TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287060
Insert Size	900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287060.1
RefSeq Size	4540 bp
RefSeq ORF	900 bp
Locus ID	27342
UniProt ID	Q9UJ41
MW	34.4 kDa
Gene Summary	RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM, Mar 2010] Transcript Variant: This variant (1) encodes isoform a. Variants 1 and 6-8 all encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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