KIAA0748 (TESPA1) (NM_014796) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC257; ITPRID3; KIAA0748
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333287 representing NM_014796. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACTGGGGGGACCAACAAGACTAGTTCAAGCATCTCAGAAATTCTGGACAAAGTGCAAGAAGATGCA GAAGATGTCCTCTTCAGTCTGGGCTTTGGCCAAGAGGATCACAAAGACACTTCTCGGATACCCGCCCGA TTTTTCACCACCCCCTCTCAGGCCAAGGGCATTGATTTCCAGCTCTTCCTGAAGTCCCAGGTGCGGAGG ATTGAAATGGAAGACCCCTGCCTCATGCTGGCCAGCAGGTTTAAGCAGGTGCAAACACTGGCTGTCACT GCTGATGCCTTCTTCTGTCTCTACTCCTATGTGTCTAAGACACCTGTCCAAAAGTTCACACCATCCCAC ATGTTCTGGAATTGCAACCATCCAACTGATGTGCCATCCATCAGGATTCTGTCCCGAGAGCCTGAACCC CAGTCACCCAGAGACCGCCTTCGGAAAGCCATCTCCAAGATGTGTCTGTACACATGCCCCCGAGACCGG CCACCACCACCCCACAACACCCCCAAAAGGAACAGTTTGGACCAAGTTGTGTTGGAAGTGATGGACAAA GTGAAAGAAGAGAAGCAGTTCCTCCAGCAAGACTCTGATTTGGGGCAATTCTCACAGGAAGATCCTGTG CCCCCTGCTGAGGGTAAGAAGTTGCCCACTTCTCCCTATCCATGTGTCTTCTGCTGTGAAGAGGAAACA CAGCAGAGGATGTCCACAGTGCTAGCACCATCCCAAACTCTGGATTCGAACCCCAAGGTACCCTGTTGC ACACATTCTCTGCCCATAGAAGATCCACAGTGGAGCACAGACCCAGCTCAGATAAGGAGAGAGCTGTGT AGTCTACCAGCCACCAATACGGAAACCCATCCAGCCAAGGATGAGACTTTCTGGAAAAGAAAGAGCAGA GCAAGAAAGAGCCTCTTCCAGAAGAATCTCATGGGCAGGAAGGTTAAGTCCTTGGACCTGTCCATCACC CAGCAGAAATGGAAGCAGAGTGTAGACAGACCAGAACTGCGTCGGTCTTTAAGCCAGCAGCCACAGGAC ACTTTTGACTTGGAGGAGGTGCAAAGCAACAGTGAGGAGGAGCAGAGTCAGAGTCGCTGGCCCAGCAGA CCCAGGCACCCCCACCACCACCAGACTTTTGCTGGCAAAGACTCGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_014796
Insert Size	1152 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014796.2
RefSeq Size	3705 bp
RefSeq ORF	1152 bp
Locus ID	9840
UniProt ID	A2RU30
MW	44 kDa
Gene Summary	Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development (By similarity). Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. May play a role in the regulation of inositol 1,4,5-trisphosphate receptor-mediated Ca(2+) release and mitochondrial Ca(2+) uptake via the mitochondria-associated endoplasmic reticulum membrane (MAM) compartment.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3), as well as variants 4 and 5, encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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