NOTO (NM_001134462) Human Untagged Clone
CAT＃: SC325670
NOTO (untagged)-Human notochord homeobox (NOTO)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325670 representing NM_001134462.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTAGCCCCAGGCCGCGAGGCAGCCCGCCACCCGCTCCCTCGGGCTCTCGGGTCCGACCTCCGCGC TCTGGCCGCTCTCCGGCGCCCAGGTCCCCTACTGGCCCGAACACGCCCCGCGCTCCCGGACGCTTCGAG TCCCCTTTCTCGGTCGAGGCCATCCTGGCGAGGCCCGACCCCTGCGCGCCGGCGGCCTCCCAGCCGTCG GGCTCCGCCTGCGTCCACCCGGCCTTCTGGACCGCTGCTTCCCTGTGCGCCACCGGGGGTCTGCCCTGG GCTTGCCCGACATCGTGGCTGCCCGCCTACCTGAGCGTAGGTTTTTACCCTGTGCCAGGGCCGCGCGTG GCTCCCGTCTGCGGCCTGCTGGGCTTCGGCGTCACAGGGTTGGAGCTGGCTCACTGCTCAGGACTCTGG GCCTTCCCAGACTGGGCCCCAACGGAGGACCTACAGGACACTGAGAGACAGCAAAAGAGAGTCCGAACT ATGTTTAACTTGGAGCAGCTGGAAGAGTTGGAGAAAGTGTTTGCAAAACAGCACAATCTGGTGGGGAAG AAGAGAGCCCAGCTGGCAGCTCGGCTCAAACTTACAGAGAACCAGGTGAGAGTCTGGTTCCAGAACCGC AGGGTCAAGTATCAGAAGCAGCAAAAGCTGAGGGCAGCAGTTACATCTGCCGAGGCTGCCTCCCTGGAT GAGCCTTCCAGCAGCTCCATCGCCAGTATCCAGAGTGATGATGCCGAGTCAGGAGTGGACGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001134462 |
| Insert Size | 756 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001134462.1 |
| RefSeq Size | 1448 bp |
| RefSeq ORF | 756 bp |
| Locus ID | 344022 |
| UniProt ID | A8MTQ0 |
| MW | 27 kDa |
| Gene Summary | Transcription regulator acting downstream of both FOXA2 and Brachyury (T) during notochord development. Required for node morphogenesis. Is essential for cilia formation in the posterior notochord (PNC) and for left-right patterning; acts upstream of FOXJ1 and RFX3 in this process and is required for the expression of various components important for axonemal assembly and function. Plays a role in regulating axial versus paraxial cell fate. Activates the transcription of ciliary proteins C11orf97 homolog, FAM183B and SPACA9 in the embryonic ventral node (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225326 | NOTO (Myc-DDK-tagged)-Human notochord homeobox (NOTO) |
CNY 2400.00 |
|
| RC225326L1 | Lenti ORF clone of Human notochord homeobox (NOTO), Myc-DDK-tagged |
CNY 4800.00 |
|
| RC225326L2 | Lenti ORF clone of Human notochord homeobox (NOTO), mGFP tagged |
CNY 5890.00 |
|
| RC225326L3 | Lenti ORF clone of Human notochord homeobox (NOTO), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC225326L4 | Lenti ORF clone of Human notochord homeobox (NOTO), mGFP tagged |
CNY 5890.00 |
|
| RG225326 | NOTO (tGFP-tagged) - Human notochord homeobox (NOTO) |
CNY 4370.00 |
