MAP3K10 (NM_002446) Human Untagged Clone
CAT＃: SC323447
MAP3K10 (untagged)-Kinase deficient mutant (K125M) of Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10)
CNY 10488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MEKK10; MLK2; MST |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323447 sequence for NM_002446 edited (data generated by NextGen Sequencing)
ATGGAGGAGGAGGAGGGGGCGGTGGCCAAGGAGTGGGGCACGACCCCCGCGGGGCCCGTC TGGACCGCGGTGTTCGACTACGAGGCGGCGGGCGACGAGGAGCTGACCCTGCGGAGGGGC GATCGCGTCCAGGTGCTTTCCCAAGACTGTGCGGTGTCCGGCGACGAGGGCTGGTGGACC GGGCAGCTCCCCAGCGGCCGCGTGGGCGTCTTCCCCAGCAACTACGTGGCCCCCGGCGCC CCCGCTGCACCCGCGGGCCTCCAGCTGCCCCAGGAGATCCCCTTCCACGAGCTGCAGCTA GAGGAGATCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGCGAG GAGGTGGCAGTCATGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAG CAGGTGTGCCAGGAAGCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTT AGGGGCGCCTGCCTCAACCCCCCACACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGT GCACTGAGCAGGGTGCTGGCAGGTCGCCGGGTGCCACCTCACGTGCTGGTCAACTGGGCT GTGCAGGTGGCCCGGGGCATGAACTACCTACACAATGATGCCCCTGTGCCCATCATCCAC CGGGACCTCAAGTCCATCAACATCCTGATCCTGGAGGCCATCGAGAACCACAACCTCGCA GACACGGTGCTCAAGATCACGGACTTCGGCCTCGCCCGCGAGTGGCACAAGACCACCAAG ATGAGCGCTGCGGGGACCTACGCCTGGATGGCGCCGGAGGTTATCCGTCTCTCCCTCTTC TCCAAAAGCAGTGATGTCTGGAGCTTCGGGGTGCTGCTGTGGGAGCTGCTGACGGGGGAG GTCCCCTACCGTGAGATCGACGCCTTGGCCGTGGCGTATGGCGTGGCTATGAATAAGCTG ACGCTGCCCATTCCCTCCACGTGCCCCGAGCCCTTTGCCCGCCTCCTGGAGGAATGCTGG GACCCAGACCCCCACGGGCGGCCAGATTTCGGTAGCATCTTGAAGCGGCTTGAAGTCATC GAACAGTCAGCCCTGTTCCAGATGCCACTGGAGTCCTTCCACTCGCTGCAGGAAGACTGG AAGCTGGAGATTCAGCACATGTTTGATGACCTTCGGACCAAGGAGAAGGAGCTTCGGAGC CGTGAGGAGGAGCTGCTGCGGGCGGCACAGGAGCAGCGCTTCCAGGAGGAGCAGCTGCGG CGGCGGGAGCAGGAGCTGGCAGAACGTGAGATGGACATCGTGGAACGGGAGCTGCACCTG CTCATGTGCCAGCTGAGCCAGGAGAAGCCCCGGGTCCGCAAGCGCAAGGGCAACTTCAAG CGCAGCCGCCTGCTCAAGCTGCGGGAAGGCGGCAGCCACATCAGCCTGCCCTCTGGCTTT GAGCATAAGATCACAGTCCAGGCCTCTCCAACTCTGGATAAGCGGAAAGGATCCGATGGG GCCAGCCCCCCTGCAAGCCCCAGCATCATCCCCCGGCTGAGGGCCATTCGCCTGACTCCC GTGGACTGTGGTGGCAGCAGCAGTGGCAGCAGCAGTGGAGGAAGTGGGACATGGAGCCGC GGTGGGCCCCCAAAGAAGGAAGAACTGGTCGGGGGCAAGAAGAAGGGACGAACGTGGGGG CCCAGCTCCACCCTGCAGAAGGAGCGGGTGGGAGGAGAGGWGAGGCTGAAGGGGCTGGGG GAAGGAAGCAAACAGTGGTCATCAAGTGCCCCCAACCTGGGCAAGTCCCCCAAACACACA CCCATCGCCCCTGGCTTCGCCAGCCTCAATGAGATGGAGGAGTTCGCGGAGGCAGAGGAT GGAGGCAGCAGCGTGCCCCCTTCCCCCTACTCGACCCCGTCCTACCTCTCAGTGCCACTG CCTGCCGAGCCCTCCCCGGGGGCGCGGGCGCCGTGGGAGCCGACGCCGTCCGCGCCCCCC GCTCGGTGGGGACACGGCGCCCGGCGGCGCTGCGACCTGGCGCTGCTAGGCTGCGCCACG CTGCTGGGGGCTGTGGGCCTGGGCGCCGACGTGGCCGAGGCGCGCGCGGCCGACGGTGAG GAGCAGCGGCGCTGGCTCGACGGCCTCTTCTTTCCCCGCGCCGGCCGCTTCCCGCGGGGC CTCAGCCCACCCGCGCGTCCCCACGGCCGCCGCGAAGACGTGGGCCCCGGCCTGGGCCTG GCGCCCTCGGCCACCCTCGTGTCGCTGTCGTCCGTGTCCGACTGCAACTCCACGCGTTCA CTGCTGCGCTCTGACAGTGACGAGGCCGCACCGGCCGCGCCCTCCCCACCACCCTCCCCG CCCGCGCCCACACCCACGCCCTCGCCCAGCACCAACCCCCTGGTGGACCTGGAGCTGGAG AGCTTCAAGAAGGACCCCCGCCAGTCGCTCACGCCCACCCACGTCACGGCTGCATGCGCT GTGAGCCGCGGGCACCGGCGGACGCCATCGGATGGGGCGCTGGGGCAGCGGGGGCCGCCC GAGCCCGCGGGCCATGGCCCTGGCCCTCGTGACCTTCTGGACTTCCCCCGCCTGCCCGAC CCCCAGGCCCTGTTCCCAGCCCGCCGCCGGCCCCCTGAGTTCCCAGGCCGCCCCACCACC CTGACCTTTGCCCCGAGACCTCGGCCGGCTGCCAGTCGCCCCCGCTTGGACCCCTGGAAA CTGGTCTCCTTCGGCCGGACACTCACCATCTCGCCTCCCAGCAGGCCAGACACTCCGGAG AGCCCTGGGCCCCCCAGCGTGCAGCCCACACTGCTGGACATGGACATGGAGGGGCAGAAC CAAGACAGCACAGTGCCCCTGTGCGGGGCCCACGGCTCCCACTAA Clone variation with respect to NM_002446.3 374 a=>t;1721 a=>w;1818 t=>c;2493 c=>t >OriGene 5' read for mutant NM_002446 unedited
CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTGCCGCCCTCGCC CTCGTCCCCCACCGGCGGACCCCGGCGGGCATTCGAGAGCCGCGCGGCCAGGCCCTCTTAGCCCTCTGCC GTTTGGGGGGCACGGGTGAACCTGCCGCCCCACTCCCACCCCGCCCCGCCCCGCCCGTACAGCCAAATCG GAAGGGACGAGCCTGCCCTTTGAAAGGGTTTTTTTTCTTGCTCCTGCGGAGGGCGCCCCAGCCATGGGCC TCAGGAGCTCCCTAGACCCCGCAGGGACTGCCCTCCATCCCGGCCGCCGGGGCCCGCCCTCTGCATCCCG CGGGCAGCTGGGTGAAGCGGCCTTCCCGCACCCCCGGCCCCCTCCCCCATGGGAGGAGAAGGAGGGGGCC G >SC323447 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYRCCWGAGGAGWCATCGGTGTGGGGGGCTTTGGCAAGGTCTATCGGGCCCTGTGGCGTGGCGAGGAGGT GGCAGTCATGGCCGCCCGGCTGGACCCTGAGAAGGACCCGGCAGTGACAGCGGAGCAGGTGTGCCAGGAA GCCCGGCTCTTTGGAGCCCTGCAGCACCCCAACATAATTGCCCTTAGGGGCGCCTGCCTCAACCCCCCAC ACCTCTGCCTAGTGATGGAGTATGCCCGGGGTGGTGCACTGAGCA |
| Restriction Sites | Please inquire |
| ACCN | NM_002446 |
| Insert Size | 3800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002446.2, NP_002437.2 |
| RefSeq Size | 3428 bp |
| RefSeq ORF | 2865 bp |
| Locus ID | 4294 |
| UniProt ID | Q02779 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218669 | MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 10480.00 |
|
| RC218669L1 | Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 12880.00 |
|
| RC218669L2 | Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 9030.00 |
|
| RC218669L3 | Lenti-ORF clone of MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 9030.00 |
|
| RC218669L4 | Lenti-ORF clone of MAP3K10 (mGFP-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 12880.00 |
|
| RG218669 | MAP3K10 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 7890.00 |
|
| SC118630 | MAP3K10 (untagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10) |
CNY 10488.00 |
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