ACCN4 (ASIC4) (NM_018674) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACCN4; BNAC4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_018674 edited GGGCTCCGGAGCACATGCTGAGCGGAGCGGCTGGGGCTGCGCGGCGTGGCGGAGCAGCGC TCGCTCCCTCGCTCACTCGCTCGCTCGCAGGGACACACGCAGGGGCTGACAGCTGTGCTG GTGCTGATAAGGGAAGCCACAAGGAGACGATCGAGGAGAGAGACAAGCGGCAGCAGAGGC AGCAGCGGCAGAGGCAGCACCAGGGCTGCGGAGCTGCTGGGAGTGGGAGTGACTCCCCCA CCTCGGGCCCCCACCCTGTCCCTGTCCTCTTCCCGCTTGCCCTGAGTTTAGAAGAGCAGC CGCTGCCACCACTGCCACTCGGGAGGGCACCAGGGCTGCTGGCTAGGGAGGGACAGGGCA GGGAGGCTCTGGCCAGTCCCAGCAGCCGGGGACAGATGCCGATCGAGATTGTGTGCAAAA TCAAATTTGCTGAGGAGGATGCGAAACCCAAGGAGAAGGAGGCAGGGGATGAGCAGAGCC TCCTCGGGGCTGTTGCCCCTGGAGCAGCCCCCCGAGACCTGGCCACCTTTGCCAGCACCA GCACCCTGCATGGACTGGGCCGGGCCTGTGGCCCAGGCCCCCACGGACTGCGCAGAACCC TGTGGGCACTGGCCCTACTCACCTCGCTGGCTGCCTTCCTGTACCAGGCGGCTGGCCTGG CCCGGGGCTACCTGACCCGGCCTCACCTGGTGGCAATGGACCCCGCTGCCCCAGCCCCAG TGGCGGGCTTCCCGGCTGTCACCCTCTGCAATATCAACCGCTTCCGGCATTCGGCACTCA GCGATGCCGACATCTTCCACCTGGCCAATCTGACAGGGCTGCCCCCCAAAGACCGGGATG GGCACCGTGCGGCTGGCCTGCGCTACCCAGAGCCTGACATGGTAGACATCCTCAACCGCA CTGGCCACCAGCTCGCCGACATGCTTAAGAGCTGCAACTTCAGTGGGCATCACTGCTCCG CCAGCAACTTCTCTGTGGTCTATACTCGCTATGGGAAGTGTTACACCTTCAACGCGGACC CGCGGAGCTCGCTGCCCAGCCGGGCAGGGGGCATGGGCAGTGGCCTGGAGATCATGCTGG ACATCCAGCAGGAGGAGTACCTGCCCATCTGGAGGGAGACAAATGAGACGTCGTTTGAGG CAGGTATTCGGGTGCAGATCCACAGCCAGGAGGAGCCGCCCTACATCCACCAGCTGGGGT TCGGGGTGTCCCCAGGCTTCCAGACCTTTGTGTCCTGCCAGGAACAGCGGCTGACCTACC TGCCCCAGCCCTGGGGCAACTGCCGCGCAGAGAGTGAGCTCAGGGAGCCTGAGCTTCAGG GCTACTCGGCCTACAGTGTGTCTGCCTGCCGGCTGCGCTGTGAAAAGGAGGCCGTGCTTC AGCGCTGCCACTGCCGGATGGTGCACATGCCAGACTCCCTGGGTGGGGGCCCTGAGGGCC CGTGCTTCTGCCCCACCCCCTGCAACCTGACACGCTATGGGAAAGAGATCTCCATGGTCA GGATCCCCAACAGGGGCTCAGCCCGGTACCTGGCGAGGAAGTACAACCGCAACGAGACCT ACATACGGGAGAACTTCCTGGTCCTAGATGTCTTCTTTGAGGCCCTGACCTCTGAAGCCA TGGAGCAGCGAGCAGCCTATGGCCTGTCAGCCCTGCTGGGAGACCTCGGGGGACAGATGG GCCTGTTCATTGGGGCCAGCATCCTCACGTTGCTGGAGATCCTCGACTACATCTATGAGG TGTCCTGGGATCGACTGAAGCGGGTATGGAGGCGTCCCAAGACCCCCCTGCGGACCTCCA CTGGGGGCATCTCCACTTTGGGGCTTCAGGAGCTGAAGGAACAGAGTCCCTGCCCGAGCC TGGGCCGAGCGGAGGGTGGGGGGGTCAGCAGTCTGCTCCCCAATCACCACCACCCCCACG GTCCCCCAGGAGGTCTCTTTGAAGATTTTGCTTGCTAGGACGGTGCTGTGACTGAAAGGA CCCAGGAGTCTGGGACCCCTCCTGGGATCCCCAGCACATTCTCCTGCTCCTGGGAGAGGC CTGGGGGCGGTGCTCACTGGGAGGGCCAGGACTCAGTTCCTGCTCTCATCCTCCCCTGCC CTGATGTCAGCTGCTTTGCACAAAGGTCCTTCTTGTCCACACCCCTTATCCCCAGGCTGG TGCCCCGGGAGGGCTGGAGACCAGGCCATGGGCCCTCACGGAGAGGAAGGGAAGGAAGGA GAGGGAGGGGGAGGATAGAGCCCATCCCAGCCGGGGAGGGGGAGCCCTCTGTACATTTGT AAATATTTAGGGAAAGCCGGGTGGGGGGAGGGGATACAGATGTAGAAGGTGGGTAGGGCT ACAGGGGTGGGTGATTTAGGGACAGCCAGGGTCCCAGCCCCAATGTCAGCAGGATAGGGA GAGCCCCAGGACTCAGGAGTGCTGGGCTGGTCCTACTTCCTGCCCCTCTCCAGGCCCAGC TCCCCTCTTGGCAGGGGGAGAGGATGGCCCAGCAGGCCTGGCCCAGCTCCCAGTTCCCCC TGCACCAGCCCCACCCCTAGAGTCCCTTCTATAGGGAGGGGGCAGGAGACCTTCCAGACT TCGGCTGAGCTTGGAGGGTGGGAAGGGAGCCTTCTCAGTCCTCTCTCCCTCCAGTCTGAT TTTATAAAGTGCTGACGAGATTGGGAATAAAGAGGCATAAAGAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_018674 unedited GTCCAAATTTTGTAAACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGACCC ACGCGTCCGGGGCTCCGGNACACATGCTGAGCGGAGCGGCTGGGGCTGCGCGGCGTGGCG GAGCAGCGCTCGCTCCCTCGCTCACTCGCTCGCTCGCAGGGACACACGCAGGGGCTGACA GCTGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCGAGGAGAGAGACAAGCGGC AGCAGAGGCAGCAGCGGCAGAGGCAGCACCAGGGCTGCGGAGCTGCTGGGAGTGGGAGTG ACTCCCCCACCTCGGGCCCCCACCCTGTCCCTGTCCTCTTCCCGCTTGCCCTGAGTTTAG AAGAGCAGCCGCTGCCACCACTGCCACTCGGGAGGGCACCAGGGCTGCTGGCTAGGGAGG GACAGGGCAGGGAGGCTCTGGCCAGTCCCAGCAGCCGGGGACAGATGCCGATCGAGATTG TGTGCAAAATCAAATTTGCTGAGGAGGATGCGAAACCCAAGGAGAAGGAGGCAGGGGATG AGCAGAGCCTCCTCGGGGCTGTTGCCCCTGGAGCAGCCCCCCGAGACCTGGCCACCTTTG CCAGCACCAGCACCCTGCATGGACTGGGCCGGGCCTGTGGCCCANGCCCCCACGGACTGC GCAGAACCCTGTGGGCACTGGCCCTACTCACCTCGCTGGCTGCCTTCCTGTACCANGCGG CTGGCCTGGCCCGGNGCTACCTGACCCGGCCTCACCTGGTGGCAATGGACCCGCTGCCCC AGCCCCAGTGGCGGGCTTCCCGGCTGTCACCCTCTGGCATATCAACCGCTTCGGGCATTT CGCACTCAGCGATGCGGACATCTTNCACCTGGCCAATCTGACAGGGCTGCCCCCCAAAGA CCGGGATGGGCACCCTGCGGCTTGGCCTGCG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_018674 unedited AGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGCATCTGTTCAGGAACAGCTA TGACCGCGGCCGCCCTTTTTTTTTTTTTTTCTTTATGCCTCTTTATTCCCAATCTCGTCA GCACTTTATAAAATCAGACTGGAGGGAGAGAGGACTGAGAAGGCTCCCTTCCCACCCTCC AAGCTCAGCCGAAGTCTGGAAGGTCTCCTGCCCCCTCCCTATAGAAGGGACTCTAGGGGT GGGGCTGGTGCAGGGGGAACTGGGAGCTGGGCCAGGCCTGCTGGGCCATCCTCTCCCCCT GCCAAGAGGGGAGCTGGGCCTGGAGAGGGGCAGGAAGTAGGACCAGCCCAGCACTCCTGA GTCCTGGGGCTCTCCCTATCCTGCTGACATTGGGGCTGGGACCCTGGCTGTCCCTAAATC ACCCACCCCTGTAGCCCTACCCACCTTCTACATCTGTATCCCCTCCCCCCACCCGGCTTT CCCTAAATATTTACAAATGTACAGAGGGCTCCCCCTCCCCGGCTGGGATGGGCTCTATCC TCCCCCTCCCTCTCCTTCCTTCCCTTCCTCTCCGTGAGGGCCCATGGCCTGGTCTCCAGC CCTCCCGGGGCACCAGCCTGGGGATAAGGGGTGTGGACAAGAAGGACCTTTGTGCAAAGC AGCTGACATCANGGCAGGGGAGGATGAGAGCAGGAACTGAGTCCTGGCCCTCCCAGTGAG CACCGCCCCCAGGCCTCTCCCAGGACAGGAGAATGTGCTGGGGATCCCAGGAGGGGTCCC AGACTCCTGGGTCCTTTCAGTCACAGCACCGTCCTAGCAAGCAAAATCTTCAAGAGACCT CCTGGGGACCTGGGGTGGTGGGTGAA
Restriction Sites	Please inquire
ACCN	NM_018674
Insert Size	2800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_018674.3, NP_061144.2
RefSeq Size	2754 bp
RefSeq ORF	2001 bp
Locus ID	55515
UniProt ID	Q96FT7
Protein Families	Druggable Genome, Ion Channels: Other
Gene Summary	This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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