SEC62 (NM_003262) Human Untagged Clone
CAT＃: SC118087
SEC62 (untagged)-Human SEC62 homolog (S. cerevisiae) (SEC62)
CNY 3656.00
CNY 7220.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Dtrp1; HTP1; TLOC1; TP-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003262, the custom clone sequence may differ by one or more nucleotides
ATGGCGGAACGCAGGAGACACAAGAAGCGGATCCAGGAAGTTGGTGAACCATCTAAAGAAGAGAAGGCTG TGGCCAAGTATCTTCGATTCAACTGTCCAACAAAGTCCACCAATATGATGGGTCACCGGGTTGATTATTT TATTGCTTCAAAAGCAGTGGACTGTCTTTTGGATTCAAAGTGGGCAAAGGCCAAGAAAGGAGAGGAAGCT TTATTTACAACCAGGGAGTCTGTGGTTGACTACTGCAACAGGCTTTTAAAGAAGCAGTTTTTTCACCGAG CCCTAAAAGTAATGAAAATGAAATATGATAAAGACATAAAGAAAGAAAAAGATAAAGGAAAAGCTGAAAG TGGAAAAGAAGAAGATAAAAAGAGCAAGAAAGAAAATATAAAGGATGAGAAGACAAAAAAAGAAAAAGAG AAAAAAAAAGATGGTGAAAAGGAAGAATCCAAAAAGGAGGAAACTCCAGGAACTCCTAAAAAGAAGGAAA CTAAGAAAAAATTCAAACTTGAGCCACATGATGATCAGGTTTTTCTGGATGGAAATGAGGTGTATGTATG GATCTATGACCCAGTTCACTTTAAAACATTTGTCATGGGATTAATTCTTGTGATTGCAGTAATAGCGGCC ACCCTCTTCCCCCTTTGGCCAGCAGAAATGAGAGTAGGTGTTTATTACCTCAGTGTGGGTGCAGGCTGTT TTGTAGCCAGTATTCTTCTCCTTGCTGTTGCTCGATGCATTCTATTTCTCATCATTTGGCTCATAACTGG AGGAAGGCACCACTTTTGGTTCTTGCCAAATCTGACTGCTGATGTGGGCTTCATTGACTCCTTCAGGCCT CTGTACACACATGAATACAAAGGACCAAAAGCAGACTTAAAGAAAGATGAGAAGTCTGAAACCAAAAAGC AACAGAAGTCCGACAGTGAGGAAAAGTCAGACAGTGAGAAAAAGGAAGATGAGGAGGGGAAAGTAGGACC AGGAAATCATGGAACAGAAGGCTCGGGGGGAGAACGGCATTCAGACACGGACAGTGACAGGAGGGAAGAT GATCGATCCCAGCACAGTAGTGGAAATGGAAATGATTTTGAAATGATAACAAAAGAGGAACTGGAACAGC AAACAGATGGGGATTGTGAAGAGGATGAGGAAGAGGAAAATGATGGAGAAACACCTAAATCTTCACATGA AAAATCATAA >OriGene 5' read for NM_003262 unedited
GGTGCAAATATTTGTATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGA ACGCAGGAGACACAAGAAGCGGATCCAGGAAGTTGGTGAACCATCTAAAGAAGAGAAGGC TGTGGCCAAGTATCTTCGATTCAACTGTCCAACAAAGTCCACCAATATGATGGGTCACCG GGTTGATTATTTTATTGCTTCAAAAGCAGTGGACTGTCTTTTGGATTCAAAGTGGGCAAA GGCCAAGAAAGGAGAGGAAGCTTTATTTACAACCAGGGAGTCTGTGGTTGACTACTGCAA CAGGCTTTTAAAGAAGCAGTTTTTTCACCGAGCCCTAAAAGTAATGAAAATGAAATATGA TAAAGGAAGACAGTGCAGCCACCTCCGAGAGCCTGGATGTGATGGCGTCACAGAAGAGAC CCTCCCAGAGGCACGGATCCAAGTACCTGGCCACAGCAAGTACCATGGACCATGCCAGGC ATGGCTTCCTCCCAAGGCACAGAGACACGGGCATCCTTGACTCCATCGGGCGCTTCTTTG GCGGTGACAGGGGTGCGCCCAAGCGGGGCTCTGGCAAGGACTCACACCACCCGGCAAGAA CTGCTCACTACGGCTCCCTGCCCCAGAAGTCACACGGCCGGACCCAAGATGAAAACCCCG TAGTCCACTTCTTCAAGAACATTGTGACGCCTCGCACACCACCNCCGTCGCAGGGAAAGG GGAGAGGACTGTCCCTGAGCAGATTTAGCTGGNGGGCCCGAAGCCAGAGACCAGGATTTG GCTACGNGAGCAGAGCGTCCGACTATTTAATCGCTCACAAGGGATTCAAGGGAGTCGATG CCCAGNGCACGCTTTCCAAAATTTTAAAGCTGGAGGAGAGAAGTCGCTCTGGATCACCAT GGCTAGACGCTGAAAACCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003262 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003262.3, NP_003253.1 |
| RefSeq Size | 6541 bp |
| RefSeq ORF | 1200 bp |
| Locus ID | 7095 |
| UniProt ID | Q99442 |
| Domains | Sec62 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Efficient secretion of small proteins in mammalian cells relies on Sec62-dependent posttranslational translocation
,null,
Molecular Biology of the Cell
,PubMed ID 22648169
[SEC62]
|
|
Efficient secretion of small proteins in mammalian cells relies on Sec62-dependent posttranslational translocation
,Asvin K. K. Lakkaraju, Ratheeshkumar Thankappan, Camille Mary, Jennifer L. Garrison, Jack Taunton, and Katharina Strub,
Mol. Biol. Cell, Jul 2012; 23: 2712 - 2722.
[SEC62]
|
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204452 | SEC62 (Myc-DDK-tagged)-Human SEC62 homolog (S. cerevisiae) (SEC62) |
CNY 3656.00 |
|
| RC204452L1 | Lenti ORF clone of Human SEC62 homolog (S. cerevisiae) (SEC62), Myc-DDK-tagged |
CNY 6056.00 |
|
| RC204452L2 | Lenti ORF clone of Human SEC62 homolog (S. cerevisiae) (SEC62), mGFP tagged |
CNY 5890.00 |
|
| RC204452L3 | Lenti ORF clone of Human SEC62 homolog (S. cerevisiae) (SEC62), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204452L4 | Lenti ORF clone of Human SEC62 homolog (S. cerevisiae) (SEC62), mGFP tagged |
CNY 5890.00 |
|
| RG204452 | SEC62 (tGFP-tagged) - Human SEC62 homolog (S. cerevisiae) (SEC62) |
CNY 4370.00 |
