Spindly (SPDL1) (NM_017785) Human Untagged Clone
CAT＃: SC113945
SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99)
CNY 4512.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCDC99 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113945 sequence for NM_017785 edited (data generated by NextGen Sequencing)
ATGGAGGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGA CTAAAAGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAA TTGGATAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATAT ACCCTTCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGT GAAGCTATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGA AGCCATGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTA GATGAAGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTC CTCTCTTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCT TCAGAGATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTC AAAGAAGAAGTGAATGAACTACAATACAGACAAGAACAGCTAGAACTTCTTATTACTAAC CTAATGCGCCAGGTAGACCGGCTTAAAGAGGAAAAAGAGGAGCGAGAGAAAGAAGCAGTT TCTTACTATAATGCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTCAGGTACAGTTG GACCAGGCACTCCAGCAAGCCTTGGATCCCAATAGTAAAGGCAACTCTTTGTTTGCAGAG GTGGAAGATCGAAGGGCAGCAATGGAACGTCAGCTTATCAGTATGAAAGTCAAGTATCAG TCACTAAAGAAGCAAAATGTATTTAACAGAGAACAGATGCAGAGAATGAAGTTACAAATT GCCACGTTGCTACAGATGAAAGGGTCTCAAACTGAATTTGAGCAGCAGGAACGGTTGCTT GCCATGTTGGAGCAGAAGAATGGTGAAATAAAACATCTTTTAGGTGAAATTAGAAATCTG GAGAAATTTAAGAATTTATATGACAGTATGGAATCTAAGCCTTCAGTCGACTCTGGTACT CTGGAAGATAACACCTATTATACAGATTTACTTCAGATGAAGCTGGATAACTTAAACAAA GAAATTGAAAGCACTAAAGGTGAATTGTCCATACAGCGAATGAAAGCATTATTTGAGAGC CAGCGGGCTCTAGATATTGAGCGAAAACTTTTTGCAAATGAAAGATGCCTCCAGCTTTCA GAAAGTGAAAATATGAAACTGAGAGCTAAACTAGATGAATTGAAACTAAAATATGAACCT GAAGAGACAGTTGAAGTGCCTGTACTGAAAAAGAGGCGTGAGGTGCTCCCTGTGGATATA ACCACCGCTAAAGATGCATGTGTCAACAACAGTGCTCTCGGGGGAGAAGTTTATCGATTA CCGCCTCAGAAAGAGGAGACACAGTCCTGCCCTAACAGTTTAGAAGATAACAACTTGCAA TTAGAAAAATCAGTTTCTATACACACACCAGTAGTCAGTCTCTCTCCTCACAAAAATCTG CCCGTGGATATGCAGCTGAAGAAGGAAAAGAAATGTGTGAAACTCATAGGAGTTCCCGCT GACGCTGAGGCCTTAAGTGAAAGAAGTGGAAACACCCCTAACTCTCCCAGGTTAGCTGCT GAATCAAAGCTTCAAACAGAAGTTAAAGAAGGAAAAGAAACTTCAAGCAAATTGGAAAAA GAAACTTGTAAGAAATCACACCCTATTCTATATGTGTCTTCTAAATCTACTCCAGAGACC CAGTGCCCTCAACAGTAA Clone variation with respect to NM_017785.4 816 c=>t;1522 t=>c;1757 t=>c >OriGene 5' read for NM_017785 unedited
GGTTACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGCGAATG GGCCGGCGACTGTGGAGTTAGCGTCCTCATGTGGACGCCCTGAGCTCCCATTAGGAGCCG CTGGCTGCGGCAGCAGGGGACTAGCGTGAGAGTTGGCTAAAAAAAAGAAAAGAACATGGA GGCAGATATAATCACAAATCTTCGATGCAGGCTCAAAGAGGCTGAAGAAGAGCGACTAAA AGCTGCACAGTATGGTTTACAACTAGTAGAGAGTCAAAATGAATTACAGAATCAATTGGA TAAATGTCGTAATGAAATGATGACCATGACTGAGAGTTATGAACAAGAAAAATATACCCT TCAAAGAGAAGTTGAACTCAAGAGTCGAATGTTAGAAAGTTTGAGCTGCGAATGTGAAGC TATTAAACAACAACAAAAAATGCACCTGGAGAAATTGGAAGAACAACTAAGCAGAAGCCA TGGACAGGAAGTGAATGAACTAAAAACTAAGATAGAAAAACTGAAAGTGGAATTAGATGA AGCCAGGCTTAGTGAAAAGCAGCTGAAGCACCAAGTAGATCATCAGAAGGAACTCCTCTC TTGTAAATCAGAGGAACTGCGCGTAATGTCTGAACGTGTGCAGGAAAGCATGTCTTCAGA GATGCTGGCTCTTCAAATTGAGCTGACAGAAATGGAGAGTATGAAGACCACCCTCAAAGA AGAAAGTGATGNAACTACATACANGACAGAACAGCTAGAACTTCTTATTACTAACCTAAT GCGCCCAGTAGACCGGCTTNANAGAGGAAAAAGAGAGCGAGAGAAAGAGCAGTTNCTTAC TATAATGCCCCTAGAGAAAGCTCGTGTAGCAAATCAAGATCTTTCAGTACAGTTGGNACC AGCACTTCACCAGCCCTGGATC >OriGene 3' read for NM_017785 unedited
TGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGAAAAAACACGGGGGTTTTTTTT AAAATTTTAAACAAAATTTAAATCTTGTTTTTAAAACCAAAAAGGAACTTTGGTTTTTTA AACCCTGACAAAATCCTGAAACAATTTAAGGTTTTTTTTTTAACCCACTTTTCCTTTTTT TACAAAAAAGTTAACACCCTTCCCTTTTTTACAACATAGTTTAAAACTAAGTTTATAAAA AAAAGTCCCTGGGAAAATTTGATGGAAGCTTTTTCAAAAACCGGGGTTTGGGGGGAAAAA ACTTGTTTTTTTATCATTTAACCCATATCCAAGGCGGCAAAAAAAACCCTTAATTATAAC CTTTCCCTTCAATAAAAAGTTTATTCCCGGGCAAAAAACCCCCATGAAGGGGCATCACTT AAAAACCTCTCCAGGTTAAAGCAAACCAAATTTTGAACACTTGGTCAAAGGCCCATAAAA ATGCCCGGCCCTGGCCCAATGAGTAAATACCCCAAAGGGGGATTTTTGTCAAATAAAAAC AATTTTAACCACCATATAAACTTTTGGGGCAAGGGGCACCGTCCTCTTTTTAAAAAAAAG TCTTTACTGTGGAGGGCACTGGGTCTCTGGAGTTAATTTAAAAAACCTTTTAGAATGGGG GGGATTTCTTACAGTTTCTTTTTCCAATTGCTAGAGGNTCCTTTCCTTCTTTACTTCCGG NGAAGCCTTGATTCACAGCTACCCTGGGAGAGTGGGGGGGGTCCCCTTTNTTTACTTAGG GCCCAACGTAAGCGGAACCTCTAAGAGTTCACCATTTTTTCCTTCTTAGTGCTTTCCCGG ACAATTTTGGAGGAAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017785 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017785.2, NP_060255.2 |
| RefSeq Size | 2561 bp |
| RefSeq ORF | 1818 bp |
| Locus ID | 54908 |
| UniProt ID | Q96EA4 |
| Gene Summary | This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling. [provided by RefSeq, Jul 2016] Transcript Variant: Variants 3 and 4 encode the same isoform (b). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203078 | SPDL1 (Myc-DDK-tagged)-Human coiled-coil domain containing 99 (CCDC99) |
CNY 4504.00 |
|
| RC203078L1 | Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged |
CNY 6904.00 |
|
| RC203078L2 | Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged |
CNY 5890.00 |
|
| RC203078L3 | Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC203078L4 | Lenti ORF clone of Human coiled-coil domain containing 99 (CCDC99), mGFP tagged |
CNY 5890.00 |
|
| RG203078 | SPDL1 (tGFP-tagged) - Human coiled-coil domain containing 99 (CCDC99) |
CNY 6104.00 |
|
| SC321078 | SPDL1 (untagged)-Human coiled-coil domain containing 99 (CCDC99) |
CNY 4512.00 |
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