RNF70 (PJA1) (NM_022368) Human Untagged Clone
CAT＃: SC108048
PJA1 (untagged)-Human praja ring finger 1 (PJA1), transcript variant 3
CNY 3656.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PRAJA1; RNF70 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108048 sequence for NM_022368 edited (data generated by NextGen Sequencing)
ATGGGTCAGGAATCTAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAGTCCAAT ACAGGTAGGAGGTATGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACGAGCCAG CGGGAAAGGATTGCCAGCCAGAGAAAGACGAACTCCGAAGTCCCAATGCACAGATCAGCC CCCAGTCAAACCACCAAGAGGAGCCGATCGCCATTTTCCACTACTCGTCGTAGTTGGGAC GACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATGAGGACNNNNNCAGTACTTCC AGGTGGAGGGATACCGCCAATGACAATGAGGGCCACTCGGATGGCCTGGCAAGAAGAGGG AGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAGTACCCTGAAGACAAACGGGAAGCG AGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGACGACGACGCACCATGGCCGACCCT GACTTCTGGACGCACAGTGATGATTACTACAAATACTGCGACGAAGACTCTGACAGTGAC AAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGAAGCCGAGAGCAAACCCTGTCCTCC AGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAAGAGCGGGAACCTCCACAGGCTAAG GTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGTGCTAGTGCCAGTGCCGGGGCTGGC GCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTACCTTGAAGAAGTTCGAGAACCATCT CTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAAATTCCTTGGCTCCAGTACCATGAG AATGACAGTAGCAGTGAGGGGGATAATGATTCTGGTCACGAGTTGATGCAACCTGGGGTA TTCATGCTGGATGGAAACAACAACCTTGAAGATGACTCCAGTGTGAGCGAAGACCTAGAA GTGGATTGGAGCCTCTTTGATGGATTTGCAGATGGGTTAGGAGTGGCTGAAGCCATTTCC TATGTGGACCCTCAGTTCCTCACCTACATGGCACTTGAAGAACGCCTGGCCCAGGCAATG GAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTGGATGTAGAGGTGGCCAATCCACCA GCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATCCTGGTCACTGAAGATCATGGCGCA GTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGCGAATATGTGAAGGGGGAGGTGGCA ACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCGTGTGTGTCCATCTGGCTTCAGAAG TCAGGCACCTGCCCCGTGTGCCGCTGCATGTTCCCTCCCCCACTCTAA Clone variation with respect to NM_022368.4 286 t=>n;287 a=>n;288 t=>n;289 t=>n;290 c=>n >OriGene 5' read for NM_022368 unedited
ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGAGTATTTGTGG TGGTGGGGAAAACACCTCAAAGAGTGCAGAGGAACCTGTGGTCAGGCCCAAAATCAGAAA CCTGGCAAGTCCAAACTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGATGACGA TATGCCACACAGTACTTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCACTCGGA TGGCCTGGCAAGAAGAGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAGTACCC TGAAGACAAACGGGAAGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGACGACG ACGCACCATGGCCGACCCTGACTTCTGGACGCACAGTGATGATTACTACAAATACTGCGA CGAAGACTCTGACAGTGACAAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGAAGCCG AGAGCAAACCCTGTCCTCCAGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAAGAGCG GGAACCTCCACAGGCTAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGTGCTAG TGCCAGTGCCGGNGCTGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTACCTTGA AGAAGTTCGAGAACCATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAANGAGAAATTCC TTGGCTCCAGTACCATGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGTCACGA GTTGATGCANCCTGNGGTATTCATGCTGGATGGAAAACACACCTTGAAGAGACTNCAGTG TGAGCGAGACCTAGAAGTGGATGGNAGCCTCTTGATGGATTGCN >OriGene 3' read for NM_022368 unedited
ACTTGCACCCGGCCGCAATCTATGATCGATTCTTTTTTTTTCTTTTTTTTTTTTTTAAAA CCAAAAAAACAACTTTAATAAGCTTTTACGGCACTGCAATTACAGGAACATCGACCCATA ACATGCAACAAAAATGATTTTGCCTTTTGGACATATTTAACAGATAAACTTGACATTACA AGTAACAGTAACACATTCCCATTCTACTGAACAAAACAAATGCGATTTAACTTCCAGGTT ACAAAACGTCTCTTCTTACTGCACTCTCAAGTAGCATTTAAAAAGCTTAGTTTTCACCTT TTCTAACCTCCAAAAGCATGATCTGATATTTAATGACCACATACACTACTGTTAACACAT TGGAAATAATCACCAGGAATCAATAGGTATAGGCTAACCCGACTGATCGAACTTATTATC CTTGCCAACACAAAGAGGACTCCTGCTTATTTGCGGATACAATATGGGGAAAACATTCTT ACCACTACCTATCGGCCTTGCATTTTAAAGTGGGGGAGGATACACCACCCCCACTCGGCG CTAGCACACGTCTACGAACACCTATGGACACACTTCACTCGAGGACAACCAGCAGTCATG CCCTCCTTTCGTCCCCATACTCTTACCATTCACCCACCCCCCACGGGGGCCATAACCTAC AAGTAATCACGTTTCTCTCCCCCCCCCCCCCCCTCCTCCTTTATCCTACTCTGCTCCTTT TCCTCCAGACGCTTTACACTATACCTCTCCTGTCTTTTTATTTTTCCCCCTGCACCCCTC CTCACCGAATTCAACTATTCCCTTCTTATCCATATGATCACCCGGCACCCCACCCCTTGA TTTTCTATTGTTAACCTAACAAACCACCACAGACGCTCCGCACCTCACGCCCCTTTATGT TATAAATCTACTGTACTTACACCACCCCTACATCCTCCTCTTTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022368 |
| Insert Size | 1730 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022368.1, NP_071763.1 |
| RefSeq Size | 1535 bp |
| RefSeq ORF | 1086 bp |
| Locus ID | 64219 |
| UniProt ID | Q8NG27 |
| Domains | RING |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010] Transcript Variant: This variant (3) lacks an in-frame segment of the 5' coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217710 | PJA1 (Myc-DDK-tagged)-Human praja ring finger 1 (PJA1), transcript variant 3 |
CNY 3656.00 |
|
| RC217710L3 | Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 3, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC217710L4 | Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 3, mGFP tagged |
CNY 5890.00 |
|
| RG217710 | PJA1 (tGFP-tagged) - Human praja ring finger 1 (PJA1), transcript variant 3 |
CNY 4370.00 |
|
| SC317746 | PJA1 (untagged)-Human praja ring finger 1 (PJA1), transcript variant 3 |
CNY 7220.00 |
