FCHO2 (AL832928) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832928, the custom clone sequence may differ by one or more nucleotides CTGCTTCATTGAGTGCTGCCAATACTCCAACAGTAGGTGTGTCACGGGGTCCCAGCCCTGTCAGCCTTGG AAATCAGGATACCTTACCTGTGGCAGTTGCCCTTACAGAATCTGTTAATGCCTACTTTAAAGGAGCAGAT CCCACCAAGTGTATTGTGAAGATCACTGGTGATATGACAATGTCATTTCCAAGTGGAATTATTAAAGTCT TCACCAGCAATCCAACTCCAGCTGTGTTGTGCTTCAGGGTGAAAAATATCAGCAGACTAGAGCAGATTCT TCCAAATGCACAGCTTGTGTTCAGTGATCCATCACAATGTGATTCCAACACAAAAGATTTTTGGATGAAC ATGCAAGCTGTTACAGTCTACCTCAAGAAGCTGTCAGAGCAAAATCCAGCTGCTTCTTATTATAATGTAG ATGTATTAAAGTATCAGGTATCATCAAATGGTATTCAGTCCACTCCTCTGAATCTTGCAACATACTGGAA ATGTAGTGCTAGCACCACAGATCTTAGAGTGGATTATAAATACAATCCAGAAGCTATGGTGGCACCTAGT GTGCTTTCCAACATACAGGTGGTGGTACCAGTGGATGGAGGAGTAACGAACATGCAGTCCCTTCCCCCTG CAATATGGAATGCAGAACAAATGAAAGCCTTTTGGAAATTGTCTAGTATTTCAGAAAAATCAGAAAATGG AGGTTCTGGGTCCCTCCGAGCAAAATTTGATCTTTCAGAAGGACCTAGTAAACCCACGACACTTGCAGTA CAATTCCTCAGCGAGGGAAGTACCCTTTCAGGAGTAGATTTCGAACTTGTGGGCACTGGCTATAGGCTTT CCTTAATAAAGAAGCGGTTTGCTACTGGACGATACCTGGCGGATTGTTGATGGACCTGGGAAAGTGATGT GGCTTCAGGGATTACAAACCTGCCATTCTGCATTATCTGTTCTTTCCAAACACTATTTTAACTTGTATGA TGTCTTTCAAACTTAGACATATTTGAGAGCTGACTACAAACATTAAATCGCACTTTTAAAGTGAGTCATT GAAATAAATAGTACTGAAATGATTCTCTATGTTGTAAATGATCAACTACAATATTCAGGAAGCACATTTA TTCAGATTCTCAGTAAAAATGAAGTTTTTGTAGGATTAATTTTTAAATTATTTCCAGTGTCTATGTTGAA AAAAAGGGTTATAGTGTAATATCAGGCCTAAAGTTTCAGAAGAGCAAGCACAAAGTAATTTAGCTTTCCA TACATTTTTAGAGTCAGAGATAACTTTGAAATTTCATAAGTTTGATATTCAGTGGATACAAATAGAACAT GAATTCTAAGTTTTGGGTAATGTTAACTCAGAACACTTAATCAAATTGAGCTATGTGCCATAAGGTAATC AGACCAGAGTCCAAGTTGTATGCAAAAAATCTATAATTTGATTCTCATAAACATATTAATAAAGTATAAG TGATGTTTTATATGATTTTTAAAAAAAATTCTCAAGTGCAATGTGTTTTAAAATAAGCTTGCAAATGACA GCAGAGACATTTACTTCTGCAGTTAGTTAACTTTATAGGAGTTGTGATTTTTTTGATGAATTAATGCTGT AGATTTAATTTATTTTTATATGGAATTGCATAATGTGTGCCTTTTAAAACAATAACAAAAACCAGAGATG TGCCTATACAATTTGTGTTCATTAATGTGCCTCACTTTTTTTCTCAGTCTGAATCTTCTGTTCAGAATCT TACAGTGATCAAGCATTTTTATAAGATATTTGCAATTTTTGTAAATATTTTTGGCCTGCAACTGGGAAGG GGATTCAGAGTCATAAAGCCTAACTTAAGAATCCTGGCTCACTTCTTTGGTGATACACTGTAGCCACTAG AAAGAGAAATAAACCCAATGGCATGTGTTTACAGGAAGAAAGATTACCTTCAGATACTTGACATTAACCT CATCAAAAGTGTGTGAATTTTAAAACAAATTCTAAAAAAACTATAGTGCTAAATTGGTAACTGGTATTTT AACTCTCAATTTAGCATTTTCCTTGATACTTTATCATTGAAAATTTATGCATTTATTTACTGATTGTGTT GTGAACATGAGTTTTATGGTATTTTGTGAGGGATGCATTTTTTAAAAGATTAATTTTGGAAACAGAAGGA GAAGCACTGCCATTTGTTTTTATTTCAACAATTGGAAAAATTACCAATTTGTACATTTTAATTATTCAAT TTCTACTTTGTAAAAATAGGGAAATTATTTGAAGTGATATATTTGGTTAAACATTTCTAAAAATAAACTA CCAATACTAAATAGTAAGAAACCTAACTTTAGTAGCAAATCTTGATTAATTGAAGAAATAAAATATTTCG GATAAGGTTTCATAATTTACAAATGGGAATTTCATTTATAATAATAAATATGAGTGTCCTTTATTTGCCT AGTAGAAGTGATAAATTGTGTTATATATGATAATTTATATAAAACCACTAATTGTTCCGTTAAGCTTCAC AGAAAATAAAACCTCTATTACTAGACATTTATGTAACATCTTGCTGTACTAAATACAGCAGAAAATCTAC TCTTTAGCAATATATAACACAGTATATGCTTTTTTATATATTCAATGTTAGCCCAAGTCTGAATTTACAT TTGACTAAAGCAGCACATAAGATAATTTGCTAAAATGCTAATCTTTACTAAATAGTGAGCTAAATTAGAA TTTCTAACTCTTATATTGTGCTCTTTTAAATGCCAATTACAGTCCCTTACTGGAATTCTAACTGTAATTT GCTTTGCAATCAAACTGCTTTTAGGGCAGCCAATATTTTATTATTGGAATTCAGACACTGTTCTTACCGT GTTTGTTTTTAATCAAATATTACCTAAAAATGTACAAATTAGTGAAATGGTTAAACTTCTGCACTTTCTT AGTTACCACAGTCTTCATACCAAGTATTGGGTACAGGTTACAATTTTGAAGCCATATGAGTTTATATTGA TTTTTTTTTTCCATTTAAAAATCCACTAATGGTGTAAAAGAGACCAGTAGATTTTCAATGGGAAATGTAC CTAGCAAGCTGGTTCTTGCTTATATATAGTGATAAACTTTGTAGCTGCCTCTTTAACCAAGAATTTGTAA ACACAACTCTAATAAATGTGAGTTTTAAAGGATTGTTATTTACTACTTTGGATTGTAATTAGAACAATGC ACATCTGTCTTCCAAGATTTTGTAAATATTTTTGATTTTTTTCATTAAATGTAAATTTTACATAAAAGTT GTGCTCTTAATAAACATGTAATATATAATTTAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832928 unedited GTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTAATGCCT ACTTTAAAGGAGCAGATCCCACCAAGTGTATTGTGAAGATCACTGGTGATATGACAATGT CATTTCCAAGTGGAATTATTAAAGTCTTCACCAGCAATCCAACTCCAGCTGTGTTGTGCT TCAGGGTGAAAAATATCAGCAGACTAGAGCAGATTCTTCCAAATGCACAGCTTGTGTTCA GTGATCCATCACAATGTGATTCCAACACAAAAGATTTTTGGATGAACATGCAAGCTGTTA CAGTCTACCTCAAGAAGCTGTCAGAGCAAAATCCAGCTGCTTCTTATTATAATGTAGATG TATTAAAGTATCAGGTATCATCAAATGGTATTCAGTCCACTCCTCTGAATCTTGCAACAT ACTGGAAATGTAGTGCTAGCACCACAGATCTTAGAGTGGATTATAAATACAATCCAGAAG CTATGGTGGCACCTAGTGTGCTTTCCAACATACAGGTGGTGGTACCAGTGGATGGAGGAG TAACGAACATGCAGTCCCTTCCCCCTGCAATATGGAATGCAGAACAAATGAAAGCCTTTT GGAAATTGTCTAGTATTTCAGAAAAATCAGAAAATGGAGGTTCTGGGTCCCTCCGAGCAA AATTTGATCTTTCAGAAGGACCTAGTAAACCCACGACACTTGCAGTACAATTCCTCAGCG AGGGAAGTACCCTTTCAGGAGTAGATTTCGAACTTGTGGGCACTGGCTATAGGCTTTCCT TAATAAGAAGCGTTTGCTACTGGACGATCCTGGCGGATTGTTGATGGACCTGNGAAAGTG ATGTGGCTTCAGGNATTACAACCTGCCATTCTGCATTATCTGTTCTTTTCAACACTATNT TAANCTGTATGATGTCTTTCAACTTAGACATATTGAGAGCTGACTACAACATTAAATCGA CTTNTAAGTGAGTCATTTGAATAATAGTACTGAATGATNTNCTTGTTGTAAATGATA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832928 unedited AATGCGTACGCCCCCAAATAGGGTTNATTTAAACCGTNNNTTTAATTANTATTTTTATTG NANTAAGANNAGGGGGGGGGTGTGGGGTTTANATTTAATGAAAAAAATCAAAAATATTTA CAAAATCTTGGAAGACAGATGTGCATTGTTCTAATTACAATCCAAAGTAGTAAATAACAA TCCTTTAAAACTCACATTTATTATAGTTGTGTTTACAAATTCTTGGTTAATGAGGAGGGG CAAAGTTTACCATTATATATAAGCAAGAACCAGCTTGCTAGGTACATTTCCCATTGAAAA TCTACTGGTCTCTTTTACACCATTAGTGGATTTTTAAATGGAAAAAAAAAATCAATATAA ACTCATATGGCTTCAAAATTGTAACCTGTACCCAATACTTGGTATGAAGACTGTGGTAAC TAAGAAAGTGCAGAAGTTTAACCATTTCACTAATTTGTACATTTTTAGGTAATACTTGAT TAAAAACAAACACGGTAAAGAACAGTGTCTGAATTCCAATAATAAAATATTGGCTGCCCT AAAAGCAGTTTGATTGCAAAGCAAATTACACTTAATATTCCAGTAAGGGACTGTAATTGG CATTTTAAAGAGCACAATATAAGAGTTAGAAATTCTAATTTAGCTGACCATTTATTAAAG ATTAGCATTTTAACAAATATCTTATGTGCGGCTTTAAACAAAGTAATTTAAACTTGGGCA ACATTGATATATAAAAAGCCTAACCGGGTTAAAATTGGCAAAAAAAAATTTTCTGCGGAA TTTATACAGCAAGATGTCCCCAAATGTCTGTAATAAAGGTTTAAATTTCTGAAAAGCTTA ACCGAACCAGTAAGGGTTTATAAAAATGCACCGAAAGAGAAAAATTAACATTTCTGCAAG GCAAACAGGGCCCCCCCCTTCCGCACACC
Restriction Sites	NotI-NotI
ACCN	AL832928
Insert Size	3600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832928.1
RefSeq Size	3335 bp
RefSeq ORF	3335 bp
Locus ID	115548
Gene Summary	Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.