ATP11C (AK091552) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATPIG; ATPIQ
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091552, the custom clone sequence may differ by one or more nucleotides ATGAAAATTTCTGGCAATGCCATGCTACAGTTGGGCCCCTTCTTATATTGGACATTTCTGGCTGCCTTTG AAGGGACAGTGTTCTTCTTTGGGACTTACTTTCTTTTTCAGACTGCATCCCTAGAAGAAAATGGAAAGGT ATACGGAAACTGGACTTTTGGAACCATTGTTTTTACAGTCTTAGTATTCACTGTAACCCTGAAGCTTGCC TTGGATACCCGATTCTGGACGTGGATAAATCACTTTGTGATTTGGGGTTCTTTAGCCTTCTATGTATTTT TCTCATTCTTCTGGGGAGGAATTATTTGGCCTTTTCTCAAGCAACAGAGAATGTATTTTGTATTTGCCCA AATGCTGTCTTCTGTATCCACATGGTTGGCTATAATTCTTCTAATATTTATCAGCCTGTTCCCTGAGATT CTTCTGATAGTATTAAAGAATGTAAGAAGAAGAAGTGCCAGGGTTCATCACTTAATTTCCTCTTCTGCAT AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091552 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAGAATAGCACAC CTTGTACAGTACTTCTTCTATAAGAACCTTTGTTTCATTTTGCCACAGTTTTTGTACCAG TTCTTCTGTGGATTCTCACAACAGCCACTGTATGATGCTGCTTACCTTACAATGTACAAT ATCTGCTTCACATCCTTGCCCATCCTGGCCTATAGTCTACTGGAACAGCACATCAACATT GACACTCTGACCTCAGATCCCCGATTGTATATGAAAATTTCTGGCAATGCCATGCTACAG TTGGGCCCCTTCTTATATTGGACATTTCTGGCTGCCTTTGAAGGGACAGTGTTCTTCTTT GGGACTTACTTTCTTTTTCAGACTGCATCCCTAGAAGAAAATGGAAAGGTATACGGAAAC TGGACTTTTGGAACCATTGTTTTTACAGTCTTAGTATTCACTGTAACCCTGAAGCTTGCC TTGGATACCCGATTCTGGACGTGGATAAATCACTTTGTGATTTGGGGTTCTTTAGCCTTC TATGTATTTTTCTCATTCTTCTGGGGAGGAATTATTTGGCCTTTTCTCAAGCAACAGAGA ATGTATTTTGTATTTGCCCAAATGCTGTCTTCTGTATCCACATGGTTGGCTATAATTCTT CTATATTTATCAGCCTGTTCCCTGAGATTCTTCTGATAGTATTAAAGAATGTAAGAAGAA GAAGTGCCAGGAATCCCGATCTTNGACTGCCTATGNTATTGTCCTACAGCATACTGACAG TGGTTACAGCTAANAAAGAAAGCATGAAGAAACCACTACCAAAAGTTATCATCTCAGGAT ACTTGATATGCCACACACTAAACCACTCTCATGTCTAGAGTCACAATANAGTTCATTAAA TACCCAATGATTCTCTTAGCATTCA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091552 unedited NNNNCGTTAAGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTT TTTTCATATCATACCCAAGTTTATTGGTTACCCCAAACAAAAATTTCTGTAATGGAAAAG GCAAGTTGCAGTCCTAAAAGATGGCCTTCACATTCATTTTAAAAAGCCACCACGTAGATG TAAAAAACTGCTTAAGTGAAAAATGTAATATTGCAGTCCCATTTTGCAAGCTGAAAAATG ATTTTGTCAACCCCCCTAAAATCTGCCCATTTATATACTGCATGTTATTAAAAAATATAT TCNCCTCCCCCCTTAAATTTATTTCACCAAATTTTTGCGAAAAGTTAGGCTCTACATTTA TACCCGGTGCTGGGGTATGGTGTAAATTGAAATGGGAAGGAAAGTTTTTCCATTTAAAAA GGGGAAACCCCCCCCAAACCAAGGGGGCCACACCGGGGTTTGGGAAAAAATTTCCCCCCC GGCCCCCCTTTTTTTGGAGGGGCCCCCCAAAATTTTTCACATTTTTGGGGGGGGGGAGGA ACCCCCAAAAATTTTTTTCCCCCCCCCCCCTCGGGGGGGCAAAAAAAAGGGGGGGGGGGA ANNNTTCTTNTTAGTAGGCAAGGGGGGCNNNCCCCCCCCCCCCCCCTTTTTATAAAAAAA AATTTTTTTNNNNNNNNNNCNNCCCCCCCCCCCCCCGCGGGGGGGGGGGTTTTTTTTTGC CCCCCCCCCCAAAAAAAAATTTTTTTTTAAAAAAAGAGAAGGACGCAACGCCCCCCCCCC CCCCCCGGGGTGTGGTGGGAGCGCGCCAGACACAAAGAAGGAAAGGTGCTAGTAGCCCCC GCCGCCCCCTAATAATAAAAAAAAGCCGCCGGGGCGGCGCACCAG
Restriction Sites	NotI-NotI
ACCN	AK091552
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091552.1, BAC03692.1
RefSeq Size	3165 bp
RefSeq ORF	3165 bp
Locus ID	286410
Protein Families	Transmembrane
Gene Summary	Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. In the cell membrane of erythrocytes, it is required to maintain phosphatidylserine (PS) in the inner leaflet preventing its exposure on the surface. This asymmetric distribution is critical for the survival of erythrocytes in circulation since externalized PS is a phagocytic signal for splenic macrophages (PubMed:26944472). Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity). Required for B cell differentiation past the pro-B cell stage (By similarity). Seems to mediate PS flipping in pro-B cells (By similarity). May be involved in the transport of cholestatic bile acids (By similarity).[UniProtKB/Swiss-Prot Function]

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