Cacna1a (NM_012918) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BccA1; Cav2.1; rbA-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204610 representing NM_012918 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTTTGGAGACGAGATGCCGGGCCGCTACGGCGCAGGCGGAGGAGGCTCAGGGCCGGCCGCCG GGGTGGTCGTGGGCGCCGCGGGCGGCCGAGGAGCCGGGGGCAGCCGGCAGGGCGGGCAGCCCGGAGCGCA GAGGATGTACAAGCAGTCGATGGCGCAGAGAGCGCGGACCATGGCCCTCTACAACCCCATCCCTGTCCGC CAGAACTGCCTCACGGTCAACCGCTCCCTGTTCCTCTTCAGTGAAGACAACGTGGTGAGAAAATACGCCA AAAAGATCACGGAATGGCCTCCCTTCGAGTACATGATCCTGGCCACCATCATTGCTAACTGCATCGTCCT GGCCCTGGAGCAGCACCTCCCTGATGATGACAAGACACCCATGTCCGAGCGGCTGGATGACACAGAACCC TATTTCATTGGCATCTTCTGTTTTGAGGCTGGAATTAAGATCGTGGCTCTTGGCTTTGCCTTCCACAAAG GCTCCTACCTGAGGAATGGCTGGAACGTCATGGACTTTGTCGTGGTGCTAACAGGCATCTTGGCCACTGT CGGGACGGAGTTTGATCTACGGACACTGAGGGCGGTTCGTGTGCTGCGGCCACTCAAGCTGGTGTCTGGA ATCCCAAGTTTACAAGTCGTCCTGAAGTCAATCATGAAGGCGATGATCCCTCTGCTGCAGATCGGCCTCC TCCTGTTTTTTGCAATCCTTATTTTTGCAATCATAGGGTTAGAATTTTATATGGGAAAATTTCATACCAC CTGCTTTGAAGAGGGGACAGACGACATCCAGGGTGAGTCGCCAGCTCCGTGTGGGACAGAGGAGCCTGCC CGCACCTGCCCCAACGGGACCAAATGTCAGCCGTACTGGGAAGGGCCCAACAACGGCATCACTCAGTTCG ACAACATCCTGTTTGCTGTGCTCACTGTTTTCCAGTGCATCACCATGGAAGGCTGGACTGATCTCCTCTA CAATAGCAACGATGCCTCAGGGAACACTTGGAACTGGTTGTACTTCATCCCCCTCATCATCATCGGCTCC TTTTTTATGCTGAACCTTGTGCTGGGTGTGCTGTCTGGGGAGTTTGCCAAAGAAAGGGAACGTGTAGAGA ACCGAAGGGCTTTTCTGAAGCTCAGAAGACAACAGCAGATTGAACGTGAGCTCAATGGATACATGGAGTG GATCTCGAAAGCAGAAGAGGTGATCCTCGCGGAGGACGAGACAGACGTGGAGCAGAGGCACCCTTTTGAT GGAGCTCTTCGGAGAGCTACTCTGAAGAAAAGCAAGACGGACCTGCTCAACCCTGAGGAGGCGGAGGACC AGCTTGCTGACATCGCGTCTGTGGGGTCTCCCTTCGCCAGAGCCAGCATCAAAAGTGCCAAGCTGGAGAA TTCGACTTTTTTCCACAAAAAGGAGAGAAGAATGCGTTTCTACATCCGCCGCATGGTCAAAACTCAGGCC TTCTACTGGACCGTGCTCAGTCTGGTAGCCCTCAACACGCTGTGGCTCGCCATTGTCCACTACAACCAGC CCGAGTGGCTCTCCGACTTCCTCTACTATGCAGAATTCATTTTCTTAGGACTCTTTATGTCCGAAATGTT TATAAAAATGTATGGGCTCGGGACACGGCCTTACTTCCACTCTTCCTTCAACTGCTTTGACTGTGGGGTC ATCATCGGGAGCATCTTTGAAGTCATCTGGGCCGTCATCAAACCGGGTACATCCTTTGGAATCAGCGTGT TACGAGCTCTCAGGTTACTGCGTATTTTCAAAGTCACAAAGTACTGGGCATCTCTCAGAAACCTGGTTGT CTCCCTCCTCAACTCCATGAAATCCATCATAAGTCTGCTGTTCCTCCTCTTCCTCTTCATTGTCGTCTTT GCCCTCTTGGGGATGCAGCTGTTTGGTGGCCAGTTTAATTTTGACGAGGGGACTCCTCCCACCAACTTCG ACACTTTTCCAGCAGCAATAATGACTGTGTTTCAGATCCTGACTGGCGAGGATTGGAATGAGGTCATGTA TGATGAGATCAAGTCTCAGGGGGGCGTGCAGGGCGGCATGGTGTTCTCCATCTACTTCATCGTCCTCACC CTCTTCGGGAACTACACCCTGCTGAACGTGTTCTTAGCTATCGCGGTGGACAACCTGGCCAACGCCCAGG AACTCACCAAGGATGAACAAGAAGAGGAAGAGGCAGCCAATCAGAAACTGGCTCTACAGAAAGCCAAGGA GGTGGCAGAAGTGAGTCCCCTGTCTGCAGCCAACATGTCCATAGCTGTGAAGGAACAGCAGAAGAACCAG AAGCCTGCCAAGTCGGTGTGGGAGCAGCGCACCAGCGAGATGCGCAAGCAGAACCTGCTGGCTAGCCGCG AGGCGCTGTACGGGGACGCGGCTGAGCGCTGGCCCACCACTTACGCGCGCCCGCTGCGGCCGGACGTGAA GACGCACTTGGACCGGCCGCTCGTGGTGGACCCGCAGGAGAACCGTAACAACAACACCAACAAGAGCCGT GCGCCAGAAGCGCTGCGCCAAACCGCGCGGCCCCGCGAGAGCGCGCGCGACCCCGACGCGCGGCGCGCCT GGCCCAGCAGCCCTGAGCGCGCCCCTGGACGAGAGGGCCCGTATGGCCGCGAGAGCGAGCCGCAACAGCG CGAGCACGCGCCACCCCGCGAGCACGTACCCTGGGACGCGGATCCTGAGCGCGCCAAGGCCGGGGACGCG CCCCGCCGCCACACGCACCGGCCTGTGGCCGAGGGCGAGCCTCGTCGCCACCGCGCGCGCCGCCGGCCCG GGGACGAACCGGACGACAGACCGGAGCGCAGGCCGCGTCCCCGCGACGCCACTAGGCCGGCCCGCGCTGC AGACGGCGAAGGCGATGATGGGGAGCGCAAGCGGCGACACCGACACGGGCCGCCGGCCCACGATGACAGG GAGCGCAGACACCGGCGCAGAAAAGAGAGCCAGGGCTCTGGGGTCCCCATGTCTGGTCCCAACCTGTCCA CCACCAGGCCAATCCAGCAGGATCTGGGCCGCCAGGACCTGCCACTGGCTGAGGACCTGGACAACATGAA GAACAACAAGTTGGCCACCGGGGAGCCTGCCAGTCCCCACGACAGCCTGGGCCACAGTGGCCTTCCCCCT AGCCCTGCCAAGATCGGGAACAGCACCAACCCTGGTCCCGCCTTGGCCACCAATCCCCAGAATGCTGCCA GCCGCAGGACGCCCAACAACCCGGGCAACCCGTCCAACCCCGGCCCCCCCAAGACTCCCGAGAACAGCCT TATCGTCACCAACCCCAGCAGCACCCAGCCCAACTCAGCAAAGACTGCCAGGAAACCCGAGCACATGGCG GTGGAGATCCCCCCGGCCTGCCCGCCCCTCAACCACACTGTGGTCCAAGTAAACAAAAACGCCAACCCAG ACCCACTGCCAAAGAAAGAGGAAGAGAAGAAGGAGGAAGAGGAGGCAGACCCCGGGGAGGATGGCCCAAA GCCCATGCCGCCCTACAGCTCCATGTTCATCCTCTCCACCACCAACCCCCTTCGCCGGCTGTGCCATTAC ATCCTGAACCTGCGCTACTTCGAGATGTGCATCCTCATGGTCATTGCCATGAGTAGCATCGCGCTGGCCG CCGAGGACCCGGTGCAGCCCAACGCACCCCGCAACAACGTGCTGCGATATTTTGACTATGTTTTCACAGG AGTGTTTACCTTTGAGATGGTGATCAAGATGATCGACCTGGGCCTCGTCCTGCATCAGGGGGCCTATTTC CGTGACCTGTGGAACATTCTGGACTTCATAGTGGTCAGTGGGGCCCTGGTGGCCTTTGCCTTCACTGGCA ATAGCAAAGGAAAGGACATCAACACCATCAAGTCCCTCCGAGTCCTCCGGGTGCTACGACCTCTAAAGAC CATCAAGCGGCTGCCTAAGTTGAAGGCCGTATTTGACTGCGTGGTGAACTCGCTCAAGAACGTCTTCAAC ATCCTCATTGTCTACATGCTCTTCATGTTCATCTTCGCCGTGGTGGCCGTGCAGCTCTTCAAGGGCAAAT TCTTCCACTGCACGGACGAGTCCAAGGAGTTTGAGAGAGACTGTCGAGGCAAATACCTCCTTTACGAGAA GAACGAGGTAAAGGCGCGGGACCGCGAGTGGAAGAAATACGACTTCCACTACGACAACGTGCTCTGGGCC CTGCTCACGCTCTTTACGGTGTCCACGGGAGAGGGCTGGCCACAGGTCCTCAAGCACTCAGTGGATGCCA CTTTTGAGAACCAGGGCCCCAGCCCCGGGTACCGCATGGAAATGTCCATCTTCTACGTGGTCTACTTTGT GGTGTTTCCCTTCTTCTTTGTCAATATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGAGAC AAGATGATGGAAGAATACAGCCTAGAGAAAAATGAGAGGGCCTGCATCGACTTTGCCATCAGTGCCAAGC CGCTGACCAGGCACATGCCCCAGAACAAGCAGAGCTTCCAGTATCGAATGTGGCAGTTCGTGGTGTCCCC ACCCTTTGAGTACACCATCATGGCCATGATCGCTCTCAACACCATCGTGCTAATGATGAAGTTCTATGGA GCCTCTGTGGCCTATGAAAACGCCCTTCGAGTGTTCAACATTGTCTTCACCTCCCTCTTCTCTCTCGAAT GTGTGCTCAAAGTCATGGCTTTTGGGATTCTGAATTATTTCCGCGATGCCTGGAACATCTTCGACTTTGT GACTGTTCTGGGCAGCATCACAGACATCCTCGTCACCGAGTTTGGGAATAACTTCATCAACCTGAGCTTT CTCCGCCTCTTCCGTGCTGCCCGACTCATCAAACTCCTCCGCCAGGGTTACACCATCCGCATTCTCCTCT GGACTTTCGTGCAGTCTTTCAAGGCCCTACCTTATGTCTGTCTGCTGATCGCCATGCTCTTCTTCATCTA TGCCATCATCGGGATGCAGGTGTTTGGCAACATCGGCATTGATGGGGAAGATGAGGACAGCGATGAGGAT GAGTTCCAAATCACGGAGCACAATAACTTCCGGACCTTCTTCCAAGCTCTCATGCTTCTCTTCCGGAGCG CCACAGGGGAAGCGTGGCACAACATCATGCTGTCCTGCCTCAGCGGGAAGCCATGCGACAAGAACTCCGG GATCCAAAAACCAGAGTGTGGCAACGAGTTCGCCTATTTTTACTTTGTCTCGTTCATCTTCCTTTGCTCA TTTCTGATGCTGAATCTCTTTGTTGCTGTCATCATGGACAACTTCGAGTACCTCACCCGAGATTCCTCCA TCCTGGGCCCCCACCACCTGGATGAGTACGTGCGTGTCTGGGCAGAGTATGACCCTGCTGCCTGCGGCCG CATTCACTATAAGGACATGTACAGTTTATTGCGAGTAATATCGCCCCCTCTCGGCTTAGGCAAGAAATGT CCTCATAGGGTTGCTTGCAAGAGGCTCTTGCGGATGGACCTACCCGTAGCGGATGACAACACCGTTCACT TCAACTCCACCTTGATGGCTCTGATCCGAACCGCCCTGGATATCAAAATCGCAAAGGGTGGAGCTGACAA GCAGCAAATGGACGCAGAGCTCCGCAAGGAAATGATGGCCATTTGGCCCAACCTGTCTCAGAAGACCTTG GATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGATGATCA TGGAGTACTACCGGCAGAGCAAGGCCAAGAAGCTGCAGGCCATGCGAGAGGAGCAGAACCGGACACCACT CATGTTCCAGCGCATGGAGCCTCCATCGCCAACACAGGAGGGAGGACCCAGCCAAAACGCCCTTCCCTCC ACTCAGCTGGACCCCGGAGGAGGCCTGATGGCTCAAGAAAGCAGCATGAAGGAGAGCCCGTCCTGGGTGA CCCAGCGGGCACAGGAGATGTTCCAGAAGACTGGTACCTGGAGCCCAGAGCGAGGGCCACCCATCGACAT GCCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGAGAGAAATGGGAACTGATGGCTACTCAGACAGC GAACACTACCTCCCCATGGAAGGACAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAACCAGA GGAGAAGGGGCCGGCCACGTGGAAATAACCTCAGTACCATCTCTGATACCAGCCCCATGAAGCGCTCAGC CTCCGTGCTGGGACCCAAAGCCCGGCGACTGGATGACTACTCACTAGAGCGGGTACCACCTGAGGAGAAC CAAAGGTACCACCAACGCCGCCGGGACCGTGGCCACCGCACCTCTGAGCGCTCTCTGGGCCGATACACTG ATGTGGACACAGGCCTGGGGACAGATCTGAGCATGACCACCCAATCGGGTGACCTGCCCTCCAAAGATCG GGACCAGGACCGGGGCCGGCCCAAGGACCGGAAGCATCGGCCACACCACCACCACCACCATCATCACCAT CATCCCCCGGCCCCGGACCGGGAGCGCTACGCACAGGAGCGGCCGGACACCGGCCGGGCGCGGGCCCGGG AGCAGCGCTGGTCCCGCTCGCCCAGCGAGGGTCGGGAGCACGCGACACACAGACAGGGCAGTAGTTCCGT CAGTGGAAGCCCAGCCCCCTCTACGTCCGGCACCAGCACGCCGCGGCGCGGCCGCCGCCAGCTCCCCCAG ACCCCCTGCACCCCGCGGCCGCTTGTGTCCTACTCGCCCGCTCCGCGCAGGCCTGCGGCGCGCAGGATGG CGGGTCCCCCAGCGCCCCCTGGCGGCTCGCCGCGGGGTTGCCGCCGTGCGCCGCGCTGGCCTGCGCATGC GCCTGAGGGCCCGCGGCCGCGCGGTGCCGACTACACCGAGCCCGACAGCCCGCGCGAGCCGCCAGGGGGC GCGCATGAGCCCGCGCCGCGGTCGCCCAGGACTCCGCGCGCCGCGGGCTGCGCGTCACCGCGCCACGGCC GCAGGCTGCCCAATGGTTACTACGCGGGGCACGGCGCGCCACGGCCGCGCACAGCCCGCAGGGGCGCGCA CGACGCGTACAGCGAGAGCGAGGACGACTGGTGCTAA CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG ATAAGGTTTAA
Restriction Sites	SgfI-XhoI
ACCN	NM_012918
Insert Size	7107 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012918.3, NP_037050.2
RefSeq Size	8912 bp
RefSeq ORF	7107 bp
Locus ID	25398
UniProt ID	P54282
Gene Summary	voltage-gated calcium channel that may be involved in secretion coupling in pancreatic beta cells [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.