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SH3BP2 (NM_003023) Human Tagged ORF Clone
CAT＃: RG205759
- TrueORF®
SH3BP2 (tGFP-tagged) - Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1
ORF Plasmid: DDK
"NM_003023" in other vectors (6)
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CNY 7,864.00
| Cited in 1 publication. |
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3BP-2; 3BP2; CRBM; CRPM; RES4-23 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205759 representing NM_003023
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGAAGAGATGCATTGGCCTGTCCCTATGAAGGCCATTGGTGCCCAGAACCTGCTAACCATGC CTGGGGGCGTGGCCAAGGCTGGCTACCTGCACAAGAAGGGCGGTACCCAGCTGCAGCTGCTGAAATGGCC CCTGCGCTTTGTCATCATCCACAAACGCTGCGTCTACTACTTCAAGAGTAGCACCTCTGCCTCCCCGCAG GGCGCCTTCTCCCTGAGTGGCTATAACCGGGTGATGCGGGCGGCTGAGGAGACCACGTCCAACAACGTTT TCCCCTTCAAGATCATCCACATCAGCAAGAAGCACCGCACGTGGTTCTTCTCGGCCTCCTCCGAGGAGGA GCGCAAGAGCTGGATGGCCTTGCTGCGCAGGGAGATTGGCCACTTCCACGAAAAGAAAGACCTGCCCTTG GACACCAGCGACTCCAGCTCGGACACAGACAGCTTCTACGGCGCAGTTGAGCGGCCTGTGGATATCAGCC TTTCCCCGTACCCCACGGACAATGAAGACTATGAGCACGACGATGAGGATGACTCCTACCTGGAGCCTGA CTCCCCGGAGCCCGGAAGGCTTGAGGATGCCCTGATGCACCCACCGGCTTACCCACCACCCCCAGTGCCC ACGCCCAGGAAGCCAGCCTTCTCTGACATGCCCCGGGCCCACTCCTTTACCTCCAAGGGCCCCGGTCCCC TACTGCCACCCCCGCCCCCTAAGCACGGCCTCCCAGATGTTGGCCTGGCTGCTGAGGACTCCAAGAGGGA CCCACTGTGCCCGAGGCGGGCTGAGCCTTGCCCCAGGGTACCTGCTACCCCCCGAAGGATGAGCGATCCC CCTCTGAGCACCATGCCCACCGCACCCGGCCTCCGGAAACCCCCTTGCTTCCGGGAGAGTGCCAGCCCCA GCCCGGAGCCCTGGACCCCTGGCCACGGGGCCTGCTCCACTTCCAGTGCTGCCATCATGGCCACTGCCAC CTCCAGAAACTGTGACAAACTCAAGTCCTTCCACCTGTCCCCCCGAGGACCACCCACATCTGAGCCCCCA CCTGTGCCAGCCAACAAGCCCAAGTTCCTGAAGATAGCTGAAGAGGACCCCCCAAGGGAGGCAGCCATGC CCGGACTCTTTGTGCCCCCCGTGGCTCCCCGGCCTCCTGCGCTGAAGCTGCCAGTGCCTGAGGCCATGGC GCGGCCCGCAGTCCTGCCCAGGCCAGAGAAGCCGCAGCTCCCGCACCTCCAGCGATCACCCCCCGATGGG CAGAGTTTCAGGAGCTTCTCCTTTGAAAAGCCCCGGCAACCCTCACAGGCTGACACTGGCGGGGACGACT CGGACGAGGACTATGAGAAGGTGCCACTGCCCAACTCGGTCTTCGTCAACACCACGGAGTCCTGCGAAGT GGAAAGGTTGTTCAAGGCTACAAGCCCCCGGGGAGAGCCCCAGGATGGACTCTACTGCATCCGGAACTCC TCTACCAAGTCGGGGAAGGTCCTGGTTGTGTGGGACGAAACCTCTAACAAAGTGAGGAACTATCGCATTT TTGAGAAGGACTCTAAGTTCTACCTGGAGGGCGAGGTCCTGTTTGTGAGTGTGGGCAGCATGGTGGAGCA CTACCACACCCACGTGCTGCCCAGCCACCAGAGCCTGCTGCTGCGGCACCCCTACGGCTACACTGGGCCT AGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205759 representing NM_003023
Red=Cloning site Green=Tags(s) MAAEEMHWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCVYYFKSSTSASPQ GAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEEERKSWMALLRREIGHFHEKKDLPL DTSDSSSDTDSFYGAVERPVDISLSPYPTDNEDYEHDDEDDSYLEPDSPEPGRLEDALMHPPAYPPPPVP TPRKPAFSDMPRAHSFTSKGPGPLLPPPPPKHGLPDVGLAAEDSKRDPLCPRRAEPCPRVPATPRRMSDP PLSTMPTAPGLRKPPCFRESASPSPEPWTPGHGACSTSSAAIMATATSRNCDKLKSFHLSPRGPPTSEPP PVPANKPKFLKIAEEDPPREAAMPGLFVPPVAPRPPALKLPVPEAMARPAVLPRPEKPQLPHLQRSPPDG QSFRSFSFEKPRQPSQADTGGDDSDEDYEKVPLPNSVFVNTTESCEVERLFKATSPRGEPQDGLYCIRNS STKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHYHTHVLPSHQSLLLRHPYGYTGP R TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003023 |
| ORF Size | 1683 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003023.2, NP_003014.2 |
| RefSeq Size | 7300 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 6452 |
| UniProt ID | P78314 |
| Domains | SH2, PH |
| Protein Families | Druggable Genome |
| Protein Pathways | Natural killer cell mediated cytotoxicity |
| Gene Summary | The protein encoded by this gene has an N-terminal pleckstrin homology (PH) domain, an SH3-binding proline-rich region, and a C-terminal SH2 domain. The protein binds to the SH3 domains of several proteins including the ABL1 and SYK protein tyrosine kinases , and functions as a cytoplasmic adaptor protein to positively regulate transcriptional activity in T, natural killer (NK), and basophilic cells. Mutations in this gene result in cherubism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Silencing of adaptor protein SH3BP2 reduces KIT/PDGFRA receptors expression and impairs gastrointestinal stromal tumors growth
,Serrano-Candelas, E;Ainsua-Enrich, E;Navinés-Ferrer, A;Rodrigues, P;García-Valverde, A;Bazzocco, S;Macaya, I;Arribas, J;Serrano, C;Sayós, J;Arango, D;Martin, M;,
Mol Oncol
,PubMed ID 29885053
[SH3BP2]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205759 | SH3BP2 (Myc-DDK-tagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1 |
CNY 6,264.00 |
|
| RC205759L1 | Lenti ORF clone of Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1, Myc-DDK-tagged |
CNY 8,664.00 |
|
| RC205759L2 | Lenti ORF clone of Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1, mGFP tagged |
CNY 8,664.00 |
|
| RC205759L3 | Lenti ORF clone of Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205759L4 | Lenti ORF clone of Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| SC128156 | SH3BP2 (untagged)-Human SH3-domain binding protein 2 (SH3BP2), transcript variant 1 |
CNY 6,272.00 |
