Nodal (NM_013611) Mouse Tagged ORF Clone
CAT#: MR226998
- TrueORF®
Nodal (Myc-DDK-tagged) - Mouse nodal (Nodal)
ORF Plasmid: tGFP
"NM_013611" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Tg.413d |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226998 representing NM_013611
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCCACAGCCTCCGCATCCTTCTTCTTCAAGCCTGTTGGGCTCTACTCCACCCGCGCGCCCCGA CCGCGGCCGCTTTGCCTCTGTGGACACGGGGGCAGCCCTCGTCACCGTCCCCTCTGGCGTACATGTTGAG CCTCTACCGAGACCCGCTGCCTCGGGCGGACATCATCCGCAGCCTCCAGGCGCAAGATGTGGACGTGACC GGACAGAACTGGACTTTCACGTTTGACTTCTCTTTTTTGAGCCAAGAAGAGGATCTGGTATGGGCGGAGC TCCGGTTGCAGCTGCCGGGCCCCATGGACATACCCACTGAGGGCCCACTCACCATTGACATTTTCCACCA GGCCAAGGGGGATCCAGAGCGGGACCCCGCTGACTGCCTGGAGCGCATTTGGATGGAGACGTTCACCGTC ATTCCTTCTCAGGTCACGTTTGCCTCAGGCAGCACAGTCCTGGAGGTGACCAAGCCACTCTCCAAGTGGC TAAAGGACCCCAGGGCACTGGAAAAGCAGGTGTCCAGTCGAGCAGAAAAGTGTTGGCATCAGCCCTACAC CCCACCTGTACCTGTCGCCAGCACCAATGTGCTCATGCTCTACTCCAACCGGCCTCAGGAGCAGAGGCAG CTAGGGGGCGCCACTTTGCTTTGGGAAGCTGAGAGCTCCTGGCGGGCCCAGGAGGGACAGCTGTCTGTAG AGAGGGGCGGATGGGGCAGAAGGCAACGCCGACATCATTTGCCAGACAGAAGCCAACTGTGTAGGAGGGT CAAGTTCCAGGTGGACTTCAACCTGATTGGCTGGGGCTCCTGGATCATCTACCCCAAGCAGTACAATGCC TATCGCTGTGAGGGCGAGTGTCCTAACCCTGTGGGGGAGGAGTTTCATCCTACCAACCATGCCTACATCC AGAGCCTGCTGAAACGATACCAACCCCACCGGGTTCCTTCCACGTGCTGTGCCCCCGTGAAGACCAAGCC ACTGAGCATGCTTTATGTGGACAATGGCAGGGTCCTCCTGGAACACCACAAGGACATGATTGTGGAGGAG TGTGGGTGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226998 representing NM_013611
Red=Cloning site Green=Tags(s) MSAHSLRILLLQACWALLHPRAPTAAALPLWTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAQDVDVT GQNWTFTFDFSFLSQEEDLVWAELRLQLPGPMDIPTEGPLTIDIFHQAKGDPERDPADCLERIWMETFTV IPSQVTFASGSTVLEVTKPLSKWLKDPRALEKQVSSRAEKCWHQPYTPPVPVASTNVLMLYSNRPQEQRQ LGGATLLWEAESSWRAQEGQLSVERGGWGRRQRRHHLPDRSQLCRRVKFQVDFNLIGWGSWIIYPKQYNA YRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLEHHKDMIVEE CGCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013611 |
ORF Size | 1062 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013611.5 |
RefSeq Size | 2094 bp |
RefSeq ORF | 1065 bp |
Locus ID | 18119 |
UniProt ID | P43021 |
MW | 40.9 kDa |
Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. Homozygous knockout mice for this gene exhibit early embryonic lethality, while expression of a hypomorphic allele results in defects in anteroposterior and left-right patterning. [provided by RefSeq, Aug 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209046 | Nodal (untagged) - Mouse nodal (Nodal), (10ug) |
CNY 3,656.00 |
|
MG226998 | Nodal (tGFP-tagged) - Mouse nodal (Nodal), (10ug) |
CNY 3,140.00 |
|
MR226998L3 | Lenti ORF clone of Nodal (Myc-DDK-tagged) - Mouse nodal (Nodal) |
CNY 4,750.00 |
|
MR226998L4 | Lenti ORF clone of Nodal (mGFP-tagged) - Mouse nodal (Nodal) |
CNY 4,750.00 |