Pan3 (BC082547) Mouse Tagged ORF Clone
CAT＃: MR208872
- TrueORF®
Pan3 (Myc-DDK-tagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S
ORF Plasmid: tGFP
"BC082547" in other vectors (4)
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CNY 4168.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700050F09Rik; A430027N15Rik; AU014670 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208872 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGGAATGTCACTGTCTGCTGGATCTTCCCCTCTCCATTCCCCCAAAATTACTCCACACACCTCTC CTGCTCCTAGAAGAAGAAGCCACACTCCAAACCCAGCAAGTTTCATGGTGCCGCCTAGTGCCTCCACTCC TGCCAATAACCCTGCTCCTCAGCCTCCGTCCTCTGGTCAGGTGATCCAGAAGGAGACTGTCGGGGGGACG ACTTACTTCTATACAGATACAACCCCAGCACCTTTGACTGGAATGGTATTTCCAAACTATCATATTTATC CTCCAACCGCACCTCATGTTGCTTATATGCAACCAAAAGCAAATGCACCTTCCTTCTTCATGGCCGACGA GCTCCGACAGGAGCTGATCAACAGACATTTAATAACGATGGCTCAAATTGACCAAGCAGATATGCCAGCA GTCCCCACAGAAGTTGACAGCTACCATAGCCTGTTCCCTCTAGAACCACTGCCACCTCCCAACCGGATAC AGAAATCAAGTAATTTTGGATATATCACGTCTTGCTACAAAGCTGTAAATAGCAAAGATGATCTGCCATA TTGCCTTCGGAGGATACATGGTTTTCGTCTTGTTAACACAAAGTGCATGGTGTTGGTTGATATGTGGAAA AAAATTCAGCACTCAAATATTGTAACTCTGCGTGAAGTGTTTACCACTAAAGCATTTGCCGAGCCTTCTC TGGTGTTCGCCTATGATTTCCATGCTGGAGGAGAAACTATGATGAGCAGACACTTTAACGACCCGAATTC TGATGCCTACTTCACAAAGAGGAAGTGGGGCCAGCACGATGGACCACTGCCCAGGCAGCATGCTGGATTG TTACCAGAGTCTCTCATCTGGGCATACATTGTACAGCTGAGTTCTGCTCTGCGGACCATTCATACAGCAG GTTTGGCCTGTAGAGTAATGGACCCAACGAAGATTCTGATAACCAGCAAAACAAGGTTACGAGTAAATTG TGTAGGAGTCTTTGATGTTTTAACATTTGATAACAGTCAAAATAATAATCCTTTGGCATTAATGGCTCAG TACCAGCAAGCAGATCTGATATCATTAGGAAAAGTTGTGTTGGCTTTGGCTTGCAACTCTTTGGCAGGAA TTCAGCGAGAAAATTTACAGAAAGCCATGGAATTGGTGACAATCAACTACTCCTCTGACCTGAAGAACCT CATCTTGTACTTGTTGACTGACCAAAACAGGATGCGGAGTGTGAATGACATCATGCCCATGATTGGTGCT CGGTTTTACACTCAATTGGATGCTGCTCAAATGAGAAATGATGTTATCGAGGAAGACCTTGCTAAGGAGG TTCAAAATGGAAGACTGTTTAGGCTTCTAGCAAAATTGGGAACAATCAACGAGAGGCCGGAGTTTCAGAA GGATCCAACCTGGTCAGAGACTGGAGACAGATACCTGTTGAAACTCTTTAGAGATCATCTTTTTCATCAG GTGACAGAAGCAGGTGCTCCCTGGATCGACCTCAGTCACATCATTTCTTGTCTTAACAAGCTAGATGCTG GTGTGCCAGAAAAAATCAGCCTGATTTCCAGAGACGAGAAGAGCGTGCTGGTGGTGACATACAGTGACCT AAAACGCTGCTTTGAAAATACCTTTCAAGAACTGATTGCGGCTGCAAATGGTAACGATCGGAACAGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208872 protein sequence
Red=Cloning site Green=Tags(s) MRGMSLSAGSSPLHSPKITPHTSPAPRRRSHTPNPASFMVPPSASTPANNPAPQPPSSGQVIQKETVGGT TYFYTDTTPAPLTGMVFPNYHIYPPTAPHVAYMQPKANAPSFFMADELRQELINRHLITMAQIDQADMPA VPTEVDSYHSLFPLEPLPPPNRIQKSSNFGYITSCYKAVNSKDDLPYCLRRIHGFRLVNTKCMVLVDMWK KIQHSNIVTLREVFTTKAFAEPSLVFAYDFHAGGETMMSRHFNDPNSDAYFTKRKWGQHDGPLPRQHAGL LPESLIWAYIVQLSSALRTIHTAGLACRVMDPTKILITSKTRLRVNCVGVFDVLTFDNSQNNNPLALMAQ YQQADLISLGKVVLALACNSLAGIQRENLQKAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGA RFYTQLDAAQMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQ VTEAGAPWIDLSHIISCLNKLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQELIAAANGNDRNSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC082547 |
| ORF Size | 1680 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC082547, AAH82547 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 1682 bp |
| Locus ID | 72587 |
| MW | 62.8 kDa |
| Gene Summary | Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217890 | Pan3 (untagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S, (10ug) |
CNY 6940.00 |
|
| MG208872 | Pan3 (tGFP-tagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S |
CNY 3520.00 |
|
| MR208872L3 | Lenti ORF clone of Pan3 (Myc-DDK-tagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S |
CNY 5130.00 |
|
| MR208872L4 | Lenti ORF clone of Pan3 (mGFP-tagged) - Mouse PAN3 polyA specific ribonuclease subunit homolog (S |
CNY 5130.00 |
