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Prkab1 (NM_031869) Mouse Tagged ORF Clone
CAT＃: MR203594
- TrueORF®
Prkab1 (Myc-DDK-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1)
ORF Plasmid: tGFP
"NM_031869" in other vectors (4)
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CNY 1,416.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300015D22Rik; AU021155; E430008F22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203594 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACACGAGCAGCGAGCGCGCCGCGCTGGAGCGGCAGGCGGGCCACAAGACGCCGCGGAGGGACA GCTCCGGGGGCGCCAAGGATGGGGACAGGCCCAAGATCCTCATGGACAGCCCTGAAGACGCCGACATCTT CCACTCCGAAGAGATCAAGGCTCCAGAGAAAGAGGAATTCCTGGCCTGGCAGCACGACCTGGAAGCGAAT GATAAAGCCCCCGCCCAGGCCCGGCCCACCGTGTTTCGATGGACAGGGGGTGGAAAGGAAGTCTACTTGT CTGGGTCCTTCAACAACTGGAGCAAGCTTCCCCTCACGAGAAGCCAGAATAACTTTGTAGCCATCCTGGA CCTGCCTGAAGGAGAGCATCAGTACAAGTTCTTCGTGGATGGACAGTGGACCCATGATCCTTCTGAGCCA ATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGTGAAGAAAACTGACTTTGAAGTATTTG ATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGATGTGTCTGAGCTGTCCAGCTCGCCCCCAGGACCCTA CCACCAGGAGCCTTACATGTCTAAACCAGAAGAGAGGTTCAAAGCCCCGCCCATCCTCCCGCCACACCTG CTGCAGGTCATCTTGAACAAGGACACGGGCATCTCTTGTGACCCAGCACTGCTTCCGGAGCCCAACCACG TCATGCTGAACCACCTCTATGCACTCTCCATCAAGGATGGAGTGATGGTGCTCAGTGCAACACACCGGTA CAAGAAAAAGTATGTCACCACCCTCCTCTACAAGCCGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203594 protein sequence
Red=Cloning site Green=Tags(s) MGNTSSERAALERQAGHKTPRRDSSGGAKDGDRPKILMDSPEDADIFHSEEIKAPEKEEFLAWQHDLEAN DKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEP IVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYMSKPEERFKAPPILPPHL LQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031869 |
| ORF Size | 813 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031869.2, NP_114075.1 |
| RefSeq Size | 2051 bp |
| RefSeq ORF | 813 bp |
| Locus ID | 19079 |
| UniProt ID | Q9R078 |
| MW | 30.3 kDa |
| Gene Summary | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
AMP kinase promotes glioblastoma bioenergetics and tumour growth
,Chhipa, RR;Fan, Q;Anderson, J;Muraleedharan, R;Huang, Y;Ciraolo, G;Chen, X;Waclaw, R;Chow, LM;Khuchua, Z;Kofron, M;Weirauch, MT;Kendler, A;McPherson, C;Ratner, N;Nakano, I;Dasgupta, N;Komurov, K;Dasgupta, B;,
Nat. Cell Biol.
,PubMed ID 29915361
[PRKAB1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207357 | Prkab1 (untagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1), (10ug) |
CNY 3,990.00 |
|
| MG203594 | Prkab1 (tGFP-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) |
CNY 2,850.00 |
|
| MR203594L3 | Lenti ORF clone of Prkab1 (Myc-DDK-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) |
CNY 4,750.00 |
|
| MR203594L4 | Lenti ORF clone of Prkab1 (mGFP-tagged) - Mouse protein kinase, AMP-activated, beta 1 non-catalytic subunit (Prkab1) |
CNY 4,750.00 |
