Alkbh7 (BC029677) Mouse Tagged ORF Clone
CAT＃: MR202335
- TrueORF®
Alkbh7 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485)
ORF Plasmid: tGFP
"BC029677" in other vectors (4)
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CNY 2400.00
CNY 2945.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310045B01Rik; 2510008E23Rik; Abh7; Spata11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202335 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGTTGCTTTCTGGGTGCGCCTGGGTGCGCGGCTCAGACTCTGCCGTGCTGGGCCGCCTGCGTGATG AGGCCGTGGTGCATCCAGGCTTCCTGAGCCAGGAGGAGGAGGACACGCTAACACGCGAACTGGAGCCCCA GCTGCGGCGCCGGCGCTACGAGTACGACCACTGGGACGCGGCCATCCATGGCTTCAGGGAGACAGAGAAA TCCTGCTGGTCTGATGCAAGCCAGGTCATCCTGCAGCGGGTTCGGGCGGCTGCATTTGGTCCTGACCAGA GCCTGCTATCCCCAGTACATGTGTTGGACCTGGAACCTCGGGGCTACATCAAGCCTCATGTTGACAGTGT CAAGTTCTGTGGATCTACCATTGCTGGCCTTTCCCTGTTGTCTCCAAGTGTTATGAAGCTGGTGCATACA CAGGAACCTGAGCAGTGGCTGGAACTGTTGCTGGAGCCAGGGTCTCTCTATATCTTAAGGGGTTCAGCCC GATATGACTTCTCCCATGAGATCCTTAGAGATGAAGAATCATTTTTTGGGGAGCACCGGGTTCCCCGGGG CCGACGCATCTCAGTGATTTGCCGCTCCCTCCCTGAGGGGATGGGGCCAGGAAGGCCAGAAGAGCCACCT CCAGCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202335 protein sequence
Red=Cloning site Green=Tags(s) MRLLSGCAWVRGSDSAVLGRLRDEAVVHPGFLSQEEEDTLTRELEPQLRRRRYEYDHWDAAIHGFRETEK SCWSDASQVILQRVRAAAFGPDQSLLSPVHVLDLEPRGYIKPHVDSVKFCGSTIAGLSLLSPSVMKLVHT QEPEQWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGEHRVPRGRRISVICRSLPEGMGPGRPEEPP PAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC029677 |
| ORF Size | 639 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC029677, AAH29677 |
| RefSeq Size | 718 bp |
| RefSeq ORF | 641 bp |
| Locus ID | 66400 |
| MW | 24.1 kDa |
| Gene Summary | May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate. Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death. ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage. Does not display DNA demethylase activity (By similarity). Involved in fatty acid metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206899 | Alkbh7 (untagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485), (10ug) |
CNY 3230.00 |
|
| MG202335 | Alkbh7 (tGFP-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) |
CNY 2850.00 |
|
| MR202335L3 | Lenti ORF clone of Alkbh7 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) |
CNY 4750.00 |
|
| MR202335L4 | Lenti ORF clone of Alkbh7 (mGFP-tagged) - Mouse alkB, alkylation repair homolog 7 (E. coli) (cDNA clone MGC:39017 IMAGE:5364485) |
CNY 4750.00 |
