Nudt16l1 (BC025569) Mouse Tagged ORF Clone
CAT＃: MR201080
- TrueORF®
Nudt16l1 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456)
ORF Plasmid: tGFP
"BC025569" in other vectors (4)
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CNY 1200.00
CNY 2945.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110001K21Rik; 5330437I08Rik; Sdos |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR201080 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACCACGACGGTTCCGGAGCTGAAACAGATCAGTCGGGAGGAAGCAATGCGCTTGGGGCCCGGCT GGAGTCATTCATGCCACGCCATGCTATACGCCGCCAACCCCGGGCAGCTTTTCGGACGTATCCCCATGCG TTTCTCAGTGCTGATGCAGATGCGCTTCGACGGGCTGCTAGGCTTTCCCGGGGGTTTCGTGGACCGGCGC TTCTGGTCGCTGGAGGATGGCTTGAATCGGGTGCTGGGCCTGGGCCTGGGCGGCCTACGCCTTACCGAAG CTGATTACCTGAGTTCACACCTGACTGAGGGTCCACACCGTGTGGTGGCACATCTGTACGCACGGCAGCT GACATTGGAACAACTGCATGCTGTGGAGATCAGCGCTGTGCACTCACGGGACCACGGTCTGGAGGTGGGG CTACTGCCTGGGGCCCGCCCCCATTCCCACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR201080 protein sequence
Red=Cloning site Green=Tags(s) MSTTTVPELKQISREEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGFVDRR FWSLEDGLNRVLGLGLGGLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQLHAVEISAVHSRDHGLEVG LLPGARPHSHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC025569 |
| ORF Size | 453 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025569, AAH25569 |
| RefSeq Size | 1354 bp |
| RefSeq ORF | 455 bp |
| Locus ID | 66911 |
| MW | 16.7 kDa |
| Gene Summary | Key regulator of TP53BP1 required to stabilize TP53BP1 and regulate its recruitment to chromatin. In absence of DNA damage, interacts with the tandem Tudor-like domain of TP53BP1, masking the region that binds histone H4 dimethylated at 'Lys-20' (H4K20me2), thereby preventing TP53BP1 recruitment to chromatin and maintaining TP53BP1 localization to the nucleus. Following DNA damage, ATM-induced phosphorylation of TP53BP1 and subsequent recruitment of RIF1 leads to dissociate NUDT16L1/TIRR from TP53BP1, unmasking the tandem Tudor-like domain and allowing recruitment of TP53BP1 to DNA double strand breaks (DSBs). Binds U8 snoRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC206907 | Nudt16l1 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456),, (10ug) |
CNY 3230.00 |
|
| MG201080 | Nudt16l1 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456) |
CNY 2850.00 |
|
| MR201080L3 | Lenti ORF clone of Nudt16l1 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456) |
CNY 4750.00 |
|
| MR201080L4 | Lenti ORF clone of Nudt16l1 (mGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456) |
CNY 4750.00 |
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