Setd2 (NM_001081340) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921524K10Rik; BC031601; KMT3A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225352 representing NM_001081340 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGCTGCCGTCGCAGCAGCCGCCACCGAAGATGGGGGATTTCTACGATCCCGAGCACCCGACCC CTGAAGAAGAAGAAAATGAGGCAAAGATTGAAAATGTGCAGAAAACAGGTTTCATCAAAGGACCAGTGTT CAAAGGTGTTGCTTCAAGTCGATTTTTGCCCAAAGGCACGAAGACAAAAGTTAATTTGGAGGAACAGGGA CGGCAAAAGGTGTCATTCAGCTTCAGTTTTACAAAGAAAACTTTACAGAATAGATTTCTCACTGCGCTTA GCAATGAAAAGCAAAGTGATTCTCCAAACTCCCCAGCTCCCCCTCTTCAAGTAGACTCAAACCCTAAAGT TAAAATGGACGCTGGAGATACTTTTCCTGCTACAGAAGAATCTTCGCCACCAAAATCGAGAGTGGAATTG GGCAGAATTCATTTTAAGAAACATTTGCTTCATGTGACATCTAGGCCACAGCTGGCTGCAAGTACAACAG CAGCATCTCCCCTTCCTCCTACAACACAGTTACCAGCAGTCTTAGCAGAGTCGATGATAGACTCACCACC TTCATCTCCACCCCCACCTCCTCCACCTCCCCAGGCCTCATCACCCTCACCACCAGCACAGATATCAGAG CCAGTGGCCTTGCCGCAACCCCCAGCAACAGCACTAATGACATCACCACCAGGACCTTTACCAGGAGACG TAGCCGTGAGAGCTCAGAAAGAATCACCAGTTAAAAGTGGACCCGAAGTTTTAGAGGTGGATACAAAGCA AGATATTGTATCTAATAGTTTGGAAGAACACACAGTTCAAACTTTGAAGGAACAAGCAGATCATCTCCTG CAAAAAGAAGATTCCCATATTGGGAAGGAAGAAGAGGTTTCAGATGGCTCTAAGATAAGCCTCAGTTCTA AAAAAGCAAGTTCTAAGAAGAAATCTTCACAATTTGAAGGCACATTTCTTGGTTCAGAGTCTGATGAAGA TTCTGTACGGACTTCTTCCAGTCAAAGATCACATGATTTAAAATCTTCAACAAGCATTGACAAGGAAAGA GATTTTAAGAAGAGCTCAGCACCTTCAAAAAGTGAGGATTTGGGGAAATCATCAAGATCGAAAACAGAGA GAGATGATAGATATTGTAGCTACTCAAAACTTGAACGAGATACTCGGTATGTATCTTCCCGATGTCGGTC CGAAAGAGATCGAAGGCGAAGCCGATCTCGTTCTAGATCTGACAGAGCCTCTAGAACTAGTTTGTCTTAT TCTCGCTCAGAAAGATCTCATTATTATGATTCTGAACGGCGCTACCATAGGAGTTCCCCTTATCGTGAGA GGACACGCTATTCTCGGCCATATACTGATAACAGGGCACGGGAGAGCTCAGACTCTGAAGATGAGTATAA GAAGACATACCCACGGCGTACCTCAGCCCATTCCTACAGAGACCTAAGGACATCATCATCTTACTCTAAA TTTGATCGGGACTGTAAAACTGAGACCTCTTACTTAGAGATGGAGAGAAGAGGAAAGTATACTTCAAAAC TAGAAAGAGAATCCAAACGGACTTCAGAGCATGAAACCATAAAAAGATGTTGTTCTCCCCCAAATGAACT GGGATTCCGACGGGGGTCATCATATTCCAAGCATGATAACAGTACTTCCCGTTATAAATCTGCCCTTTCA AAATCTATATCCAAGAATGATAAATTTAAAAATTCTTTCTGTTGTACAGAATTAAATGAGGAAAACAAAC AATCTCATTCGTTTAGTTTGCAGACTCCTTGTTCAAAAGGTAGTGAATTAAGAACAATTAATAAGATTTC TGAAAGAGAAAAGACTGGGTCTCCAACTCCATCAAATCAATTAAACGATTCACCTACTTTTAAAAAGCTA GATGAATCTCCTGTTCTTAAGCCTGAATTTATAGGACACGATGGCCGTGAAAGTATTAAAGAATTGGAGT TATCAAAAGTGAAAAATGATCAATTAAGAAATTTTTGTTCCATTGAATTAAATGTAAACGGATCTCCAGA GACAGAAGCTGATGTGGCAACATTTTGTACTTCTAAGACAGATGCTATATCAATGACTTCTGATGATAGT GTGACTGGATCAGAGGTATCCCCTTTGATCAAGGCTTGCATGCTTTCATCAAATGGATTTCAGAATGTTG GTAGATGCAGAGAAAGGGACTCAGATGATACTTGTAGGCAGCATAATACGTCAAAAAGTCCATTTAGGGA AATGGAACCTTTGTTGTCACCACACCATGATAAACTCATGTCTTTGCCAGTTAAGACTATAGATTATCCC AAAACATTAATTAAAGAACCAGTTGATAAGAGACATTCTTGCTGCAAAACCAAAGATTCAGATATATACT GTTCTCCAAATGAAAACCCTGAAGCTGAGAACGCTGAACCTTCAGCTATGACGATTTCTTCACATAGCTT TGTGAATGTGCATTTGGAATCCAAGACAGTTATATGTGATAATAGGGAGCCGACAGACCGGCACTCAGAG AATACATGTGATGAATATAAGCAGAGCATTGGTAGCACTAGTTCAGCTTCTCATAACCATTTTGATGGTT TGTATGAGCCTATAGGGAGTTCAGGTATTTCATCTCTCCAGAGTCCTCCGTCAGGAATAAGATGTGAAGA AAACACATCTCCAACTCTAGATGCAGTGGAGAGTAAAAAAGGCATAGATTTTTTAAAGTATGCACGGAAA GAAACAGATGTTGGTAGTGCCCTTCCTGATTCAGGAAAAGGATTTTCTTGGGAAAACAGGCATAATAATG TGTTATCTGGGCAGTCTTTGCAAGAGGCTCAAGAAGAAGGGAATTCCATATTGCATGAGAGAAGAGGAAG ACCAGAAATCCCCTTAGATGAGGAACAAAGAGGCCATACACATATTTCTGATGATTCAGAAGTGGTATTT CCTTATGATTTGAACTTGACCATGGAAGACAGTGATGGTATAACCTACACCTTAAAATGTGATAGTAGTG GAAATGCTCCAGAGATTGTATCTACTGTCCATGAAGACTATTCTGGATCTTCTGCAAGTTCAAGCGATGA AAGTGATTCTGAAGATACAGAGTCTGATGATAGCAGTATTCCAAGAAACCGACTCCAGTCTGTTGTGGTT GTGCCAAAGAATTCTACTTTGCCCATGGAAGAGACAAGTCCCTGTTCTTCTCGGAGCAGTCAGAGCTACA AACATTATTCTGACCGCTGGGAAGATGGATTAGAGACCAGGAGACATGCATATGAGGAAGAGTATGAGAG TAAAGGCTGTTCTCAAACTGAAAAATACTTCCTTCATAAAGGAACAGAGAGAAGTGCAGAAAGTTGTTAT TCACAGTTTGGCAGGAAAGCAGATAATCACCTGCCTGACATTGCTCATGCTCAGAGTGACGGGGTTGATA GTACAAGTCAGACAGACTCGAGATCTGACCATCTAGGTCACCTGAATCCAGAGGACACATTAAGAGCCAA AACATCTAGGCCACAAGAGCTACCAGTTTATTCTGACGATTTTGAAGATCTCCCAAATAAGTCTCGGCAG CAGATGATTTTCTCTAATCGGCCAGATAGTAGTAGACTAGGAAAAACAGAGCTGAGTTTTTCTTCCTCTT GTGACATTTCCCGAATGGATGGCTTGCACTCATCAGAAGAGCTCAGAAACCTAGGGTGGGACTTTTCCCA ACAGGAAAGGCCCACCACCACATACCAGCAGCCTGACAGCAGCTATGGAACCTGTGGTACACATAAGTAT CAACAAAGTACTGAACACTATGGTGGGACCCATAATTACTGGCAAGGCAATGGCTATTGGGATCCAAGAT CAGCAGGTAGACCTCCAGGAACTGGGCTTGCTTATGATCGAATCCAAGGGCAAGTACCAGATTCTCTAAC AGACGATCGTGAAGAAGAGGAACATTGGGATCAACGAAGTGGATCACATTTTTCAAGCCCGTCCAATAAA TTTTTCTTCCATCAGAAGGACAAGGGATCAGTGCAAGCACCGGAAATAAGCAGCAATTCAATTAAAGACG CTTTAGTTATGAACGAAAGGAAAGATTTTTCGAAAAACTTTGAAAAAAATGATATAAAAGAGAGAGGGCC TCCTAAAAAACGAAGGCAAGAGTTGGAGAGTGATTCTGAAAGTGATGGTGAACTACAGGCTAGAAAGAAA GTTAGAGTGGAGATGGAGCAGGGAGAATCATCTGTGCCCCAGCACTCAGAACTGATGGGGCCTTCGTGTG CTATGGATGACTTCAGGGACCCACAGCGGTGGAAAGAATTTGCCAAGCTGGGGAAGATGCCATGTTACTT TGATCTTATTGAAGAAAATGTTTATTTAACAGAAAGGAAGAAGAACAAATCCCACCGGGATATTAAGCGA ATGCAGTGTGAGTGTACACCTCTTTCTAAGGATGAAAGAGCTCAAGGTGAAGTAGCATGTGGAGAAGATT GCCTTAATCGTCTCCTCATGATTGAATGTTCCTCTCGATGTCCAAATGGAGATTACTGTTCAAACAGACG GTTTCAGAGAAAACAGCATGCAGATGTAGAAGTCATACTTACAGAAAAGAAAGGCTGGGGCTTAAGGGCT GCTAAGGATCTTCCTTCGAACACCTTTGTCCTGGAATACTGTGGAGAGGTACTTGATCATAAAGAGTTTA AAGCTCGGGTGAAAGAATATGCACGGAACAAAAACATCCACTACTACTTCATGGCCCTGAAAAATGACGA GATAATAGATGCCACTCAAAAAGGGAATTGCTCTCGTTTCATGAATCATAGCTGTGAACCAAACTGTGAA ACCCAGAAATGGACTGTGAATGGACAGCTGAGGGTTGGATTTTTTACCACCAAACTAGTTCCTTCAGGCT CAGAATTAACTTTTGACTACCAGTTCCAAAGATATGGCAAAGAAGCTCAGAAGTGTTTCTGTGGGTCAGC CAACTGCCGGGGCTACTTGGGAGGAGAAAACAGAGTCAGTATCAGAGCTGCAGGAGGGAAGATGAAAAAG GAACGCTCTCGAAAGAAGGATTCAGTGGATGGAGAACTTGAAGCACTGATGGAAAATGGTGAAGGCCTCT CTGATAAGAACCAGGTTCTTAGTTTATCCCGGCTCATGGTTAGAATTGAAACTTTGGAACAGAAACTTAC CTGTCTTAAGCTCATTCAGAACACACACTCACAGTCCTGCCTCAAGTCATTTCTGGAACGTCATGGGTTG TCACTGTTGTGGATCTGGATGGCAGAGCTTGGCGACGGCCGGGAAAGTAACCAGAAGCTTCAGGAAGAGA TTATAAAGACTTTGGAGCATTTGCCCATTCCTACTAAAAATATGTTGGAAGAAAGCAAAGTACTTCCAAT TATTCAGCGCTGGTCTCAAACTAAGACTGCTGTTCCTCAGTTAAGTGAAGGAGACGGGTATTCTAGTGAG AATACATCACGTGCTCACACACCGCTTAATACACCGGACCCTTCTGCCAAGCCGAGCACGGAAATGGATA CAGATACTCCCAAGAAACTCATATTCCGCAGACTGAAGATTATAAGTGAAAATAGCATGGACAGTGCAGT CTCGGACGTCACTAGTGAGCTAGAATGCAAGGATGGCAAAGAGGACCTTGATCAGTTAGAGACTGTCACT GTGGAAGAGGATGAAGAGCTGCAGTCCCAGCAGCTCCTCCCACAGCAGCTGTGCGAGTCCAAAGTTGAGA GTGAAGCCACTATTGAAGTCAGTAAGTTACCCACATCTGAACCGGAGGCAGACACGGAGACAGAGCCCAA AGACAGCAATGGCACAAAACTAGAAGAAACTATTGCTGAGGAAACACCATCCCAAGATGAAGAAGAGGGA GTGTCTGATGTTGAAAGTGAGAGAAGCCAGGAGCCACCAGATAAAACAGTGGATATAAGTGATTTGGCTA CCAAGTTACTAGACAGTTGGAAAGATCTAAAGGAGGTGTATCGGATTCCAAAGAAAAGTCAAACTGAAAA GGAGAGCACAGTAGCTGAACGAGGAAGAGATGCTGCTGCCTTCAGAGATCAAACAGCTCCAAAGACTCCT AACAGGTCTAGAGAGAGAGACCCAGACAAGCAGTCTCAAAATAAAGAGAAAAGGAAACGACGGGGCTCTC TGTCACCACCCTCTTCTGCATATGAGCGGGGAACAAAAAGGCCAGATGACAGATATGATACACCAACTTC TAAAAAGAAAGTACGAATTAAAGACCGAAACAAACTTTCTACAGAGGAGCGCAGGAAGTTGTTTGAACAA GAGGTGGCACAGAGGGAAGCTCAGAAGCAACAGCAACAGATGCAGAACTTGGGGATGACATCACCACTCC CCTTTGACTCTCTGGGATATAATGCCTCTCATCACCCCTTTGCTGGGTACCCACCAGGTTACCCCATGCA AGCCTATGTGGATCCCAGCAACCCTAATGCTGGAAAGGTGCTTCTGCCCACACCTAGCATGGACCCTGTG TGCTCCCCTGCTCCTTATGATCACGCTCAGCCCTTGGTAGGACATTCTACAGAATCCCTTGCTGCTCCCC CATCTGTGCCAGTGGTGCCGCATGTGGCAGCCTCTGTGGAAGTTTCCAGTTCTCAGTATGTAGCTCAGAA TGAAAGTGTTGTACACCAAGACTCCAATGTTCCTGTAATGCCAGTACAAGCTCCAGGCCCAGTCCAAGGA CAGAATTACAATGTCTGGGAGTCAAACCAACAGTCTGTCAGTGTACAACAGCAGTATTCTCCTGCACAAT CTCAAACAACCATATATTATCAAGGACAGACATGTTCAACTGTCTATAGTGTGACCTCGCCTTATTCACA GACAACTCCTCCAATTGTGCAGAGTTATGCCCAGCCAAGTCTTCAGTATATCCAGGGACAGCAGATTTTC ACAGCTCACCCACAAGGAGTGGTGGTACAGCCAACTGCAGCCGTGACTTCAATAGTTGCACCAGGGCAGC CTCAGTCCTTACAGCCACCTGAAATGGTTGTAACAAATAATCTACTGGACCTGCCACCACCCTCCCCTCC AAAACCAAAAACGATTGTTTTACCTCCCAACTGGAAGACAGCCCGAGACCCTGAGGGGAAGATCTACTAC TACCACGTGATCACAAGACAGACTCAGTGGGATCCTCCTACTTGGGAAAGCCCAGGAGATGATGCCAGCC TTGAGCATGAAGCTGAAATGGACCTGGGAACCCCAACCTATGATGAAAACCCCATGAAGACATCAAAAAA GCCCAAGACAGCAGAAGCAGACACCTCCAGTGAGCTGGCAAAGAAAAGCAAAGAAGTATTCAGAAAAGAG ATGTCTCAGTTCATTGTCCAGTGCCTGAATCCTTACCGGAAACCTGACTGCAAGGTGGGCAGGATCACCA CCACTGAAGATTTCAAGCACCTCGCCCGAAAGCTGACTCATGGAGTTATGAATAAGGAGCTGAAGTACTG TAAGAACCCCGAGGACCTGGAGTGCAATGAGAATGTGAAACACAAAACCAAGGAGTACATCAAGAAGTAC ATGCAGAAGTTTGGGGCTGTTTACAAACCTAAAGAGGACACTGAACTAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081340
Insert Size	7614 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081340.2, NP_001074809.2
RefSeq Size	8350 bp
RefSeq ORF	7614 bp
Locus ID	235626
UniProt ID	E9Q5F9
Gene Summary	Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:18157086, PubMed:20133625). Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation (PubMed:18157086, PubMed:20133625). Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A (By similarity). Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction (By similarity). Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR) (By similarity). Acts as a tumor suppressor (By similarity). H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A (By similarity). H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase (By similarity). Required during angiogenesis (PubMed:20133625). Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3 (PubMed:25242323). In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1 (PubMed:27518565). Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3); alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling (PubMed:27518565). Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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