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Enpp1 (NM_008813) Mouse Untagged Clone
CAT＃: MC222520
Enpp1 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), (10ug)
CNY 11,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4833416E15Rik; AI428932; C76301; CD203c; E-N; E-NPP 1; E-NPP1; Ly-4; Ly-41; M6S1; N; NPP1; Npps; P; PC-; PC-1; Pca; Pca-1; Pd; Pdnp1; ttw; twy |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222520 representing NM_008813
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGACGGCGACCAGGCAGGGCACGGGCCCCGGCACGGGTCAGCGGGAAACGGTCGCGAGCTCG AGTCACCAGCCGCGGCTTCGCTGCTCGCGCCCATGGACCTAGGAGAGGAGCCGCTGGAGAAGGCGGAGCG TGCCCGCCCTGCCAAAGACCCCAACACCTACAAAGTGCTGTCGCTGGTTTTGTCAGTATGTGTGCTAACA ACAATTCTTGGTTGTATATTTGGGTTGAAACCAAGCTGCGCCAAAGAAGTAAAAAGTTGCAAAGGCCGCT GCTTTGAAAGGACGTTCAGCAACTGTCGCTGTGATGCTGCCTGTGTCAGCCTTGGGAACTGCTGTCTGGA TTTCCAGGAGACCTGTGTGGAACCAACACATATATGGACTTGCAACAAATTCAGGTGCGGTGAGAAGAGG CTGTCCAGGTTCGTGTGCTCCTGTGCAGACGACTGCAAAACCCACAATGACTGTTGCATCAACTACAGTT CTGTGTGCCAAGATAAGAAGAGTTGGGTAGAAGAAACATGTGAAAGCATCGATACCCCGGAGTGTCCAGC AGAGTTTGAATCACCCCCTACTCTCTTGTTTTCTTTGGATGGATTCAGAGCTGAGTATTTGCACACCTGG GGTGGACTTCTTCCTGTCATTAGCAAGCTGAAAAACTGTGGAACATACACTAAAAACATGAGGCCTATGT ACCCTACCAAGACGTTTCCCAATCATTACAGCATCGTCACAGGACTTTATCCGGAATCCCATGGCATAAT TGATAACAAGATGTATGATCCCAAAATGAATGCTTCTTTCTCGCTTAAAAGTAAAGAGAAATTCAACCCT TTGTGGTACAAAGGACAGCCGATTTGGGTGACCGCTAATCATCAGGAGGTCAAGTCCGGCACATACTTTT GGCCAGGATCAGATGTGGAGATTGACGGGATTCTACCAGATATCTACAAAGTGTATAATGGGTCAGTACC ATTTGAAGAAAGGATTTTAGCTGTTCTTGAGTGGCTGCAGCTTCCTAGCCATGAAAGACCACACTTTTAC ACTCTGTATTTAGAAGAACCAGATTCTTCAGGGCATTCACATGGACCAGTCAGCAGCGAGGTCATCAAGG CCTTGCAGAAGGTTGACCGCCTGGTCGGCATGCTGATGGACGGCCTGAAGGACCTGGGCTTGGATAAATG CCTGAACCTCATCCTCATTTCAGATCACGGCATGGAACAAGGCAGCTGTAAGAAGTATGTGTACCTGAAT AAGTACTTGGGGGATGTGAACAATGTGAAAGTTGTGTATGGACCTGCTGCTCGGTTGAGACCCACTGATG TTCCAGAGACATACTATTCATTTAACTATGAAGCCCTTGCAAAAAATCTTTCTTGCCGGGAGCCAAACCA GCATTTCCGGCCTTACCTGAAACCCTTCTTACCCAAGCGCTTACACTTCGCTAAAAGTGACCGGATTGAG CCCCTGACCTTCTACCTGGACCCTCAGTGGCAACTTGCGTTGAATCCATCAGAGAGGAAATATTGTGGAA GTGGATTTCATGGCTCTGACAACTTGTTTTCAAACATGCAAGCTCTTTTTATTGGCTATGGACCTGCCTT CAAGCATGGTGCTGAAGTTGACTCCTTTGAAAATATTGAAGTCTATAACTTAATGTGTGACTTACTGGGT TTGATCCCAGCTCCCAATAATGGAAGTCATGGCAGCCTTAACCACCTTCTAAAGAAACCCATTTACAACC CAAGTCATCCCAAAGAAGAGGGCTTCCTGTCTCAGTGTCCAATCAAATCAACATCCAATGACCTCGGCTG CACATGTGACCCTTGGATTGTGCCAATCAAGGACTTTGAGAAACAGCTTAATCTGACCACAGAAGATGAT GACATTTACCATATGACTGTACCCTACGGGAGGCCCCGGATTCTACTGAAGCAGCACCGCGTCTGTCTAC TCCAGCAGCAACAGTTCTTGACCGGATACAGCCTGGACCTCCTCATGCCCCTCTGGGCGTCTTACACCTT CCTCAGTAATGACCAATTCTCCAGAGATGACTTTTCCAACTGTCTGTACCAGGATCTTCGGATTCCCCTT AGTCCTGTGCATAAATGTTCCTATTACAAAAGCAATTCTAAGCTAAGTTATGGGTTCCTCACGCCACCGA GACTAAATAGAGTTTCAAATCACATATACTCTGAAGCGCTGCTTACATCTAATATAGTGCCAATGTATCA GAGTTTTCAAGTTATATGGCACTACCTTCATGACACCCTACTGCAAAGGTATGCCCATGAAAGGAACGGC ATCAATGTTGTCAGCGGTCCCGTGTTTGACTTTGATTATGACGGACGCTATGATTCCTTAGAGATCCTGA AACAAAACAGCAGAGTCATCCGAAGCCAGGAAATTCTGATTCCGACTCACTTCTTCATTGTGCTCACCAG CTGCAAGCAGCTGTCTGAGACTCCCTTGGAGTGCTCCGCCTTAGAGTCCTCAGCTTACATACTGCCTCAT AGGCCTGATAACATTGAGAGCTGTACGCATGGGAAGCGGGAGTCTTCATGGGTCGAGGAGTTGCTGACCT TGCACAGAGCTCGGGTCACAGACGTGGAACTCATCACTGGCCTCAGCTTCTACCAGGACCGACAAGAGTC AGTTTCAGAACTGCTGAGGTTGAAAACACATTTGCCAATCTTCAGCCAAGAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008813 |
| Insert Size | 2718 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008813.4, NP_032839.3 |
| RefSeq Size | 6791 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 18605 |
| Gene Summary | This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227498 | Enpp1 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), (10ug) |
CNY 8,650.00 |
|
| MR227498 | Enpp1 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) |
CNY 6,632.00 |
|
| MR227498L3 | Lenti ORF clone of Enpp1 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) |
CNY 9,032.00 |
|
| MR227498L4 | Lenti ORF clone of Enpp1 (mGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) |
CNY 9,790.00 |
