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Enpp1 (NM_008813) Mouse Tagged ORF Clone
CAT＃: MR227498
- TrueORF®
Enpp1 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1)
ORF Plasmid: tGFP
"NM_008813" in other vectors (4)
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CNY 6,632.00
CNY 7,890.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4833416E15Rik; AI428932; C76301; CD203c; E-N; E-NPP 1; E-NPP1; Ly-4; Ly-41; M6S1; N; NPP1; Npps; P; PC-; PC-1; Pca; Pca-1; Pd; Pdnp1; ttw; twy |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227498 representing NM_008813
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCGACGGCGACCAGGCAGGGCACGGGCCCCGGCACGGGTCAGCGGGAAACGGTCGCGAGCTCG AGTCACCAGCCGCGGCTTCGCTGCTCGCGCCCATGGACCTAGGAGAGGAGCCGCTGGAGAAGGCGGAGCG TGCCCGCCCTGCCAAAGACCCCAACACCTACAAAGTGCTGTCGCTGGTTTTGTCAGTATGTGTGCTAACA ACAATTCTTGGTTGTATATTTGGGTTGAAACCAAGCTGCGCCAAAGAAGTAAAAAGTTGCAAAGGCCGCT GCTTTGAAAGGACGTTCAGCAACTGTCGCTGTGATGCTGCCTGTGTCAGCCTTGGGAACTGCTGTCTGGA TTTCCAGGAGACCTGTGTGGAACCAACACATATATGGACTTGCAACAAATTCAGGTGCGGTGAGAAGAGG CTGTCCAGGTTCGTGTGCTCCTGTGCAGACGACTGCAAAACCCACAATGACTGTTGCATCAACTACAGTT CTGTGTGCCAAGATAAGAAGAGTTGGGTAGAAGAAACATGTGAAAGCATCGATACCCCGGAGTGTCCAGC AGAGTTTGAATCACCCCCTACTCTCTTGTTTTCTTTGGATGGATTCAGAGCTGAGTATTTGCACACCTGG GGTGGACTTCTTCCTGTCATTAGCAAGCTGAAAAACTGTGGAACATACACTAAAAACATGAGGCCTATGT ACCCTACCAAGACGTTTCCCAATCATTACAGCATCGTCACAGGACTTTATCCGGAATCCCATGGCATAAT TGATAACAAGATGTATGATCCCAAAATGAATGCTTCTTTCTCGCTTAAAAGTAAAGAGAAATTCAACCCT TTGTGGTACAAAGGACAGCCGATTTGGGTGACCGCTAATCATCAGGAGGTCAAGTCCGGCACATACTTTT GGCCAGGATCAGATGTGGAGATTGACGGGATTCTACCAGATATCTACAAAGTGTATAATGGGTCAGTACC ATTTGAAGAAAGGATTTTAGCTGTTCTTGAGTGGCTGCAGCTTCCTAGCCATGAAAGACCACACTTTTAC ACTCTGTATTTAGAAGAACCAGATTCTTCAGGGCATTCACATGGACCAGTCAGCAGCGAGGTCATCAAGG CCTTGCAGAAGGTTGACCGCCTGGTCGGCATGCTGATGGACGGCCTGAAGGACCTGGGCTTGGATAAATG CCTGAACCTCATCCTCATTTCAGATCACGGCATGGAACAAGGCAGCTGTAAGAAGTATGTGTACCTGAAT AAGTACTTGGGGGATGTGAACAATGTGAAAGTTGTGTATGGACCTGCTGCTCGGTTGAGACCCACTGATG TTCCAGAGACATACTATTCATTTAACTATGAAGCCCTTGCAAAAAATCTTTCTTGCCGGGAGCCAAACCA GCATTTCCGGCCTTACCTGAAACCCTTCTTACCCAAGCGCTTACACTTCGCTAAAAGTGACCGGATTGAG CCCCTGACCTTCTACCTGGACCCTCAGTGGCAACTTGCGTTGAATCCATCAGAGAGGAAATATTGTGGAA GTGGATTTCATGGCTCTGACAACTTGTTTTCAAACATGCAAGCTCTTTTTATTGGCTATGGACCTGCCTT CAAGCATGGTGCTGAAGTTGACTCCTTTGAAAATATTGAAGTCTATAACTTAATGTGTGACTTACTGGGT TTGATCCCAGCTCCCAATAATGGAAGTCATGGCAGCCTTAACCACCTTCTAAAGAAACCCATTTACAACC CAAGTCATCCCAAAGAAGAGGGCTTCCTGTCTCAGTGTCCAATCAAATCAACATCCAATGACCTCGGCTG CACATGTGACCCTTGGATTGTGCCAATCAAGGACTTTGAGAAACAGCTTAATCTGACCACAGAAGATGAT GACATTTACCATATGACTGTACCCTACGGGAGGCCCCGGATTCTACTGAAGCAGCACCGCGTCTGTCTAC TCCAGCAGCAACAGTTCTTGACCGGATACAGCCTGGACCTCCTCATGCCCCTCTGGGCGTCTTACACCTT CCTCAGTAATGACCAATTCTCCAGAGATGACTTTTCCAACTGTCTGTACCAGGATCTTCGGATTCCCCTT AGTCCTGTGCATAAATGTTCCTATTACAAAAGCAATTCTAAGCTAAGTTATGGGTTCCTCACGCCACCGA GACTAAATAGAGTTTCAAATCACATATACTCTGAAGCGCTGCTTACATCTAATATAGTGCCAATGTATCA GAGTTTTCAAGTTATATGGCACTACCTTCATGACACCCTACTGCAAAGGTATGCCCATGAAAGGAACGGC ATCAATGTTGTCAGCGGTCCCGTGTTTGACTTTGATTATGACGGACGCTATGATTCCTTAGAGATCCTGA AACAAAACAGCAGAGTCATCCGAAGCCAGGAAATTCTGATTCCGACTCACTTCTTCATTGTGCTCACCAG CTGCAAGCAGCTGTCTGAGACTCCCTTGGAGTGCTCCGCCTTAGAGTCCTCAGCTTACATACTGCCTCAT AGGCCTGATAACATTGAGAGCTGTACGCATGGGAAGCGGGAGTCTTCATGGGTCGAGGAGTTGCTGACCT TGCACAGAGCTCGGGTCACAGACGTGGAACTCATCACTGGCCTCAGCTTCTACCAGGACCGACAAGAGTC AGTTTCAGAACTGCTGAGGTTGAAAACACATTTGCCAATCTTCAGCCAAGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227498 representing NM_008813
Red=Cloning site Green=Tags(s) MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVLSLVLSVCVLT TILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKR LSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTW GGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNP LWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFY TLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLN KYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIE PLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLG LIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDD DIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPL SPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNG INVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPH RPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008813 |
| ORF Size | 2715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008813.4, NP_032839.3 |
| RefSeq Size | 3224 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 18605 |
| MW | 103.5 kDa |
| Gene Summary | This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Antagonistic interactions between osterix and pyrophosphate during cementum formation.
,null,
Bone
,PubMed ID 31059863
[Enpp1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222520 | Enpp1 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), (10ug) |
CNY 11,690.00 |
|
| MG227498 | Enpp1 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), (10ug) |
CNY 8,650.00 |
|
| MR227498L3 | Lenti ORF clone of Enpp1 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) |
CNY 9,032.00 |
|
| MR227498L4 | Lenti ORF clone of Enpp1 (mGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1) |
CNY 9,790.00 |
