Arhgap8 (BC024991) Mouse Untagged Clone
CAT＃: MC218531
Arhgap8 (untagged) - Mouse Rho GTPase activating protein 8 (cDNA clone MGC:32512 IMAGE:5065129), (10ug)
CNY 6940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC32512 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC024991
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCGCCCAGCCTCAGTGACCTGGGCAAGAGGGAGCCGGGCGCGGCGGGTACGGACGAGCGGGGCA CGCAGCAGCGCCGGGCCTGCGCCAACGCCACCTGGAACAGTATCCATAACGGAGTGATCGCGGTCTTCCA GCGCAAGGGGCTGCCCGACCAGGAGCTCTTCATCCTCAACGAGGGCGTCCGGCAGCTGCTGAAGACGGAG CTGGGGTCCTTCTTCACTGAGTACCTGCAGAACCAGCTGCTCACGAAGGGCATGGTGATTCTTCGTGACA AGATTCGCTTCTACGAAGGACAGAAGCTGCTGGACTCATTGGCGGAGACCTGGGATTTCTTCTTCAGTGA TGTGCTGCCAACCCTGCAGGCCATCTTCTATCCTGTGCAGGGCAAGGAGCCGTCGGTGCGCCAGCTCGCC CTGCTGCATTTCCGGAACACCATCACCCTCAGCGTGAAGCTGGAGGACGCGCTGGCCCGCTCTCACGCGC GTGTTCCCCCTGCCATCGCACAGATGCTGCTGGTACTGCAGGGGGTACATGAGTCCAGGGGTGTGACTGA AGACTATCTGCGCCTCGAGACACTGATTCAGAAGGTGGTGTCGCCGTACCTGGGCACCTATGGTCTCTAT TCCAATGAAGGACCTTGTACTCATTCCTGTATCCTCGAGAAACGATTCCTACGCCGCTCCCGCTCTGGGG ATATCCTAGCCAAGAACCCAGTGGTGCGCTCCAAAAGCTACAACACTCCCCTGCTCAATCCTGTGGCCGA GCATGAAGCAGAAGGCACAGCAGCCAGCGGCACGAGCATCCGCAGACACTCAGTCTCCGAGATGACATCT TGTCCGGAGCCCCAGGGCTTTGTGGACACGCCTGGCCAGGGCCCTTCGGGGACCTTCCGGTCTTCTCCAA CACCCCACTCAGGGCCTTGCCCCAGCAGACTATACCCCCCTGCCCATTCCCCGGAACAGGGCCCAGGCCA TGGCTCCCCATCCACCTCCAGCCCAGAGACTCTTGTGGACCAGATCCTGGAGTCCGCGGACTCAGACTCG GAAGGGATATTCATTGACTTTGGGCGGGGAAGTCGCTCCAGTGTGTCTGACTTTGAGGCACCGGGAGGCC GGCCAAGTGTTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC024991 |
| Insert Size | 1137 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC024991, AAH24991 |
| RefSeq Size | 1731 bp |
| RefSeq ORF | 1136 bp |
| Locus ID | 109270 |
| Gene Summary | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205893 | Arhgap8 (tGFP-tagged) - Mouse Rho GTPase activating protein 8 (cDNA clone MGC:32512 IMAGE:5065129) |
CNY 4180.00 |
|
| MR205893 | Arhgap8 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 8 (cDNA clone MGC:32512 IMAGE:5065129) |
CNY 3800.00 |
|
| MR205893L3 | Lenti ORF clone of Arhgap8 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 8 (cDNA clone MGC:32512 IMAGE:5065129) |
CNY 5700.00 |
|
| MR205893L4 | Lenti ORF clone of Arhgap8 (mGFP-tagged) - Mouse Rho GTPase activating protein 8 (cDNA clone MGC:32512 IMAGE:5065129) |
CNY 5700.00 |
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