Ogdh (NM_010956) Mouse Untagged Clone
CAT＃: MC201038
Ogdh (untagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10ug)
CNY 10696.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC013670 sequence for NM_010956
GGCGCTTGACACCCGGAGCTCGCGGCCGAGGGTACTTGTGGAAGGCTTCAGGACAAAATGTTTCATTTAA GGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTTCACAAAACAAACC AGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGAACCATTTCTTAGT GGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAAAGTGTACATAAGT CATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACCAGAGCCCCCTTTC CCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACAACCTAACGTCGAC AAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGATACGAGGGCACCATGTAG CACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTGACATTATCTCATC CACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCACC ACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTACT GCCAGCACATTGGTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGTT TGAGACCCCTGGAATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATCC ACCAGGTTTGAGGAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAGG TGCTGATCCCTGCCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGGG GATGCCACACAGAGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTGT CAGTTTGACTCAAAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTATC ACCGCAGGATCAACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAGA GGCTGCTGACCCTGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAAA AAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCTTTTGCTGGCCAGGGCATCGTATATGAGACCTTCC ATCTCAGCGACTTGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCTT CACCACAGACCCTCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCCC ATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGAA ACACCTTCCACAAGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACGA ACCTATGTTTACACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGCA GAATTGCTAGTCTCCCAGGGTGTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAGA TCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCCC CTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGAG GATGTCTTGTTCCACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGGC TGAGCCGGATCTTGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGTA CATGGCATTTGGCTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGGC ACCTTCAGCCATCGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAACC ACCTTTGGCCAAATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCTT TGAGCTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAAC AACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGCA TTGTGCTCCTGCTGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGTT TCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCAG CTCTATGACTGCAACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGAC AGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGGC AAGAACTAGCTTTGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGCA GCTCAGGACCCACACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGAG AGCGCAAAGCCAGGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCTT TGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAAG AACCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGGT ATGCTGGCCGAGACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGCG CTTTCTGGACACAGCCTTTGACCTGGACGCATTCAAGAAATTCTCTTAGATGCTCCTGGAGTTGATGAGG CCATGGCCCCCATGTCCATGACGCTCTTTGCTTCTCAACTAAAGAATAGTGCCTCAGCACTGTCCACACG TCCCTTCGCTGTGCCACACCACCCCTGTTCTCATAGGAATTAAGTTGTCCACTGCAGTGCTCAGCTGCTC CCCGGTCACATGCTGCCCAGCCTGTGCCGACTTCTCTCAGGCTGCACACCGTTCATGGAGACCGGAAGGA GCAGAATAAGGAAAGGGCCCCTCTCAGGACATCCTAGAGAAGGAAGGCAGCTCTGGCCCCACCCATGCCC CCAGTGCAATCCTCCAGGGTAGGAACAGAACCCTATGTGGCTTCCCAGGGTACTAGCACTCAGCCCTCGT CACCCATCAAGTCGCAGATTCAAGGCCAGGAGTAGTTTCATCTTGCTAGGGCCAAGCTGAGAGCTCATGG AGGAACTATAGCTGCCAGGATTTGGGAGTCATCAGGATGTTGTGTGAATAGAGATTGTCATGGGGTATTT AGAGGACTTTAGCAGTGATGTTAGTCTAGCCCTGCTACCCTTCTTGGGTTTGGGCTGTATGTGGGAAACT TACCCCAGCTACCACGCCTGGAGAGCTTGGCTCTGAGTACGGCCCAGAAGCTCCATTGGCTCCCAACGCC AGGCACTGCTGCCTCTTGGTCCTGCTGCCTCTGCTCTCCTGACCCCTCCCCAGTCACTTCATTTTCTCTG TTGTTCCCTTGAACACACAGAAGCTGTTGACGAATTCTTTTTTTTGCTGTGCCAAGGCAGGTCAAAAGCA GATCAGTGGATAAGAGCAAGTTGTCCCAAGGAGCCAGCTGTCCTTCCTCCCTCTTTTGACCTCCACTGGG ACACACCTGATTTATTTATTTTGGTTAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010956 |
| Insert Size | 3072 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC013670, AAH13670 |
| RefSeq Size | 4035 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 18293 |
| UniProt ID | Q60597 |
| Gene Summary | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in a region of the coding sequence, compared to variant 1. The resulting isoform (3) has a shorter and different internal segment, compared to isoform 1. Variants 3 and 4 encode the same isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211465 | Ogdh (tGFP-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh) |
CNY 11210.00 |
|
| MR211465 | Ogdh (Myc-DDK-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 10688.00 |
|
| MR211465L3 | Lenti ORF clone of Ogdh (Myc-DDK-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 12070.00 |
|
| MR211465L4 | Lenti ORF clone of Ogdh (mGFP-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 12070.00 |
