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Ogdh (NM_010956) Mouse Tagged ORF Clone
CAT＃: MG211465
- TrueORF®
Ogdh (tGFP-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh)
ORF Plasmid: DDK
"NM_010956" in other vectors (4)
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CNY 11,210.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211465 representing NM_010956
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGATACGA GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG ACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCA CTTACCCACCACCACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTG GAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGA TCCGACAGAAGTTTGAGACCCCTGGAATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAG GCTTGTACGATCCACCAGGTTTGAGGAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTG GAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACT ATGTGATCATGGGGATGCCACACAGAGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGA GCAAATATTCTGTCAGTTTGACTCAAAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCAC CTGGGCATGTATCACCGCAGGATCAACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACC CTTCCCATCTAGAGGCTGCTGACCCTGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGA CACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCTTTTGCTGGCCAGGGCATCGTA TATGAGACCTTCCATCTCAGCGACTTGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACA ACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGT GGTGAATGCCCCCATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCA GCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACA ATGAGATGGACGAACCTATGTTTACACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACT GCAGAAGTATGCAGAATTGCTAGTCTCCCAGGGTGTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCC AAGTATGATAAGATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACT GGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCAC TGGCCTGGAGGAGGATGTCTTGTTCCACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACT ATCCATGGAGGGCTGAGCCGGATCTTGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGG CCCTGGCAGAGTACATGGCATTTGGCTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGA TGTGGAGCGGGGCACCTTCAGCCATCGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGC ATCCCCATGAACCACCTTTGGCCAAATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACG GTGTCCTGGGCTTTGAGCTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTT TGGTGACTTCAACAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTG CGGCAGAATGGCATTGTGCTCCTGCTGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCC GCCCAGAGCGGTTTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTT TGACATCAATCAGCTCTATGACTGCAACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCAT GTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGC GCCACCCTGAGGCAAGAACTAGCTTTGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGA AAATGGACCTGCAGCTCAGGACCCACACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTAT GACCTCACCCGAGAGCGCAAAGCCAGGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTAT CACCATTCCCCTTTGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCA GGAAGAGCACAAGAACCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCT AAGCCTGTCTGGTATGCTGGCCGAGACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGA CAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCTGGACGCATTCAAGAAATTCTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211465 representing NM_010956
Red=Cloning site Green=Tags(s) MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPK SVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIR GHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRL EMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGL EGCEVLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYH LGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIV YETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVA AEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEIS KYDKICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDVLFHIGKVASSVPVENFT IHGGLSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTC IPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWV RQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLQEENFDINQLYDCNWIVVNCSTPGNFFH VLRRQILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPENGPAAQDPHKVKRLLFCTGKVYY DLTRERKARNMEEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRA KPVWYAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010956 |
| ORF Size | 3069 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010956.4, NP_035086.2 |
| RefSeq Size | 6630 bp |
| RefSeq ORF | 3072 bp |
| Locus ID | 18293 |
| UniProt ID | Q60597 |
| Gene Summary | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201038 | Ogdh (untagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 10,696.00 |
|
| MR211465 | Ogdh (Myc-DDK-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 10,688.00 |
|
| MR211465L3 | Lenti ORF clone of Ogdh (Myc-DDK-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 12,070.00 |
|
| MR211465L4 | Lenti ORF clone of Ogdh (mGFP-tagged) - Mouse oxoglutarate dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein |
CNY 12,070.00 |
