CD14 Goat Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, PEP-ELISA, WB
Recommend Dilution	Western blot: 1-3µg/ml. Primary incubation 1 hour at room temperature. Approx 55kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the immunizing peptide. Flow Cytometry: 10ug/ml. Flow cytometric analysis of A549 cells.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Isotype	IgG
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CD14 molecule
Database Link	NP_000582 Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]
Synonyms	CD14 antigen; CD14 molecule; monocyte differentiation antigen CD14
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.