CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]

Specifications

Product Data
Clone Name	MEM-166
Applications	FC, IP, WB
Recommend Dilution	Western blot (Non-reducing conditions). Immunoprecipitation. Flow Cytometry: Use 10µl of 1/50-1/100 diluted CD177 antibody to label 106 cells in 100µl.
Reactivity	Human, Monkey, Rhesus Monkey
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes CD177 (Neutrophil Glycoprotein NB1). Antibodies against NB1 can be involved in neonatal alloimmune neutropenia (NAN). MEM-166 recognizes CD177 under Non-Reducing Conditions.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD177 molecule
Database Link	Entrez Gene 57126 Human UniProt ID Q8N6Q3
Background	CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population.
Synonyms	NB1, PRV1, HNA-2a, HNA2a
Reference Data

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