CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: MEM-M6/1]

Specifications

Product Data
Clone Name	MEM-M6/1
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 10 μg/ml. Immunoprecipitation. Western blot (under non-reducing conditions): 1 µg/ml. Immunohistochemistry on Paraffin Sections: 10 μg/ml. Positive tissue: testis.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of Human IgG1 (CD147Rg is secreted by transfectants as a dimer)
Specificity	The antibody recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	basigin (Ok blood group)
Database Link	Entrez Gene 682 Human UniProt ID P35613
Background	CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.
Synonyms	Basigin, BSG, EMMPRIN, TCSF
Reference Data

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