CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: MEM-M6/1]

Specifications

Product Data
Clone Name	MEM-M6/1
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells or 100 µl whole blood. Immunoprecipitation. Western Blot (under non-reducing conditions). Immunohistochemistry on Paraffin Sections: 10 µg/ml.  This Basigin antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD147Rg, consisting of the cDNA coding for the hinge region, CH2 and CH3 domains of Human IgG1.
Specificity	This antibody recognises the CD147 cell surface antigen.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	basigin (Ok blood group)
Database Link	Entrez Gene 682 Human UniProt ID P35613
Background	CD147 is a 50-60kD glycoprotein also known as neurothelin and basigin. CD147 is broadly expressed on peripheral blood cells and by endothelial cells. Expression upon T cells is increased following activation.
Synonyms	Basigin, BSG, EMMPRIN, TCSF
Reference Data

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