beta 2 Microglobulin (B2M) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/2,000-1/10,000 Western blot: 1/500-1/2,000 Immunohistochemistry on Paraffin or Frozen sections: 1/200-1/1,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta-2-microglobulin from Human urine
Specificity	This antibody detects Human Beta-2-microglobulin. Cross reactivity against Beta-2-microglobulin from other sources is unknown. Immunoelectrophoresisgive a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Human b2-Microglobulin.
Formulation	0.02M Potassium phosphate, 0.15M Sodium chloride Label: HRP State: Purified State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	beta-2-microglobulin
Database Link	Entrez Gene 567 Human UniProt ID P61769
Background	Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms	B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data

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