beta 2 Microglobulin (B2M) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, EM, IHC, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Beta-2-Microglobulin [Human Urine].
Specificity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes Delipidation, Salt Fractionation and Ion Exchange Chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin (Human Urine). Cross reactivity against beta-2-Microglobulin from other sources is unknown.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% Gentamicin Sulfate Label: Horseradish Peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	5.0 mg/mL by UV absorbance at 280 nm
Purification	Multistep Process
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	beta-2-microglobulin
Database Link	Entrez Gene 567 Human UniProt ID P61769
Background	Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms	B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.