DDX58 Mouse Monoclonal Antibody [Clone ID: OTI6C1]

Specifications

Product Data
Clone Name	OTI6C1
Applications	IF, IHC, WB
Recommend Dilution	WB 1:4000, IHC 1:150, IF 1:100
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human DDX58(NP_055129) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	106.4 kDa
Gene Name	DExD/H-box helicase 58
Database Link	NP_055129 Entrez Gene 23586 Human UniProt ID O95786
Background	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]
Synonyms	RIG-I; RIGI; RLR-1
Reference Data
Protein Pathways	Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway

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