TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]

Specifications

Product Data
Clone Name	OTI10H5
Applications	IHC, Simple Western, WB
Recommend Dilution	WB 1:5000~10000, IHC 1:50; Simple Western 1:20-1:50
Reactivity	Human, Dog, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	134.3 kDa
Gene Name	transient receptor potential cation channel subfamily M member 4
Database Link	NP_060106 Entrez Gene 484385 DogEntrez Gene 719120 MonkeyEntrez Gene 54795 Human UniProt ID Q8TD43
Background	Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.
Synonyms	hTRPM4; LTrpC4; PFHB1B; TRPM4B
Reference Data
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane

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