Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: AX.14]

Specifications

Product Data
Clone Name	AX.14
Applications	FC
Recommend Dilution	Flow Cytometry. Monitoring of Mucosa-associated T Lymphocytes.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts with Intestinal Lymphocytes (HML-1).
Isotype	IgG1
Formulation	PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha E
Database Link	Entrez Gene 3682 Human UniProt ID P38570
Background	The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in Peripheral blood and lymphoid organs.
Synonyms	Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Note	Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.