Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: AX.14]

Specifications

Product Data
Clone Name	AX.14
Applications	FC
Recommend Dilution	Flow Cytometry. Monitoring of Mucosa-associated T Lymphocytes.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts with Intestinal Lymphocytes (HML-1).
Isotype	IgG1
Formulation	PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	integrin subunit alpha E
Database Link	Entrez Gene 3682 Human UniProt ID P38570
Background	The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in Peripheral blood and lymphoid organs.
Synonyms	Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Note	Protocol: PBMC: Add 10 µl of MAB/10e6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

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