IGF1 Receptor (IGF1R) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7G11]

Specifications

Product Data
Clone Name	7G11
Applications	WB
Recommend Dilution	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Icluded Positive Control: Cell lysate from untreated A431 cells (See Protocols for more details).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to KLH.
Specificity	This antibody specifically recognizes the C-terminus of IGF1 receptor.
Formulation	1ml 2 x PBS State: Purified State: Lyophilized purified IgG fraction Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Predicted Protein Size	97 kDa
Gene Name	insulin like growth factor 1 receptor
Database Link	Entrez Gene 3480 Human UniProt ID P08069
Background	The IGF1 receptor (IGF1R) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The IGF1 receptor is activated upon binding of the peptide hormones IGF1 and 2, leading to autophosphorylation of tyrosine residue 1131, 1135, and 1136 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residue 950 and 1316 regulate the assembly of signal transduction complexes.
Synonyms	IGF-I receptor, IGF1 Receptor
Note	Protocol: Positive Control Cell Lysate A431 Untreated Formulation: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data

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